{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T20:35:23Z","timestamp":1793824523975,"version":"4.6.1"},"reference-count":67,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T00:00:00Z","timestamp":1630368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Borne Foundation"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Inflammation is thought to play a pivotal role in the onset of term and some forms of preterm labour. Although, we recently found that myometrial inflammation is a consequence rather than a cause of term labour, there are several other reproductive tissues, including amnion, choriodecidua parietalis and decidua basalis, where the inflammatory stimulus to labour may occur. To investigate this, we have obtained amnion, choriodecidual parietalis and decidua basalis samples from women at various stages of pregnancy and spontaneous labour. The inflammatory cytokine profile in each tissue was determine by Bio-Plex Pro\u00ae cytokine multiplex assays and quantitative RT-PCR. Active motif assay was used to study transcription activation in the choriodecidua parietalis. Quantitative RT-PCR was use to study the pro-labour genes (<jats:italic>PGHS-2<\/jats:italic>,<jats:italic>PGDH<\/jats:italic>,<jats:italic>OTR and CX43<\/jats:italic>) in all of the tissues at the onset of labour and<jats:italic>oxytocin (OT)<\/jats:italic>mRNA expression in the choriodecidual parietalis and decidua basalis. Statistical significance was ascribed to a P value &lt;0.05. In the amnion and choriodecidua parietalis, the mRNA levels of various cytokines decreased from preterm no labour to term no labour samples, but the protein levels were unchanged. The choriodecidua parietalis showed increase in the protein levels of IL-1\u03b2 and IL-6 in the term early labour samples. In the amnion and decidua basalis, the protein levels of several cytokines rose in term established labour. The multiples of the median derived from the 19-plex cytokine assay were greater in term early labour and term established labour samples from the choriodecidua parietalis, but only in term established labour for myometrium. These data suggest that the inflammatory stimulus to labour may begin in the choriodecidua parietalis, but the absence of any change in prolabour factor mRNA levels suggests that the cytokines may act on the myometrium where we observed changes in transcription factor activation and increases in prolabour gene expression in earlier studies.<\/jats:p>","DOI":"10.1371\/journal.pone.0256545","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T17:57:23Z","timestamp":1630432643000},"page":"e0256545","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Is there an inflammatory stimulus to human term labour?"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5130-9679","authenticated-orcid":true,"given":"Natasha","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronwen","family":"Herbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garvin","family":"Sooranna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5694-9915","authenticated-orcid":true,"given":"Nishel M.","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananya","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suren R.","family":"Sooranna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark 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Yulia","year":"2016","journal-title":"Endocrinology"},{"key":"pone.0256545.ref028","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/j.1471-0528.1976.tb00810.x","article-title":"A study of the elasticity and tension of fetal membranes and of the relation of the area of the gestational sac to the area of the uterine cavity","volume":"83","author":"E Parry-Jones","year":"1976","journal-title":"Br J Obstet Gynaecol"},{"key":"pone.0256545.ref029","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jri.2017.08.003","article-title":"Damage-Associated molecular pattern markers HMGB1 and cell-Free fetal telomere fragments in oxidative-Stressed amnion epithelial cell-Derived exosomes","volume":"123","author":"S Sheller-Miller","year":"2017","journal-title":"J Reprod Immunol"},{"key":"pone.0256545.ref030","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.cyto.2008.06.009","article-title":"Differences in amniotic fluid and maternal serum cytokine levels in 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colony-enhancing factor (PBEF\/Visfatin) and Interleukin-8 in amniotic epithelial cells","volume":"31","author":"CE Kendal-Wright","year":"2010","journal-title":"Placenta"},{"key":"pone.0256545.ref034","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.placenta.2007.12.008","article-title":"Chronic stretching of amniotic epithelial cells increases pre-B cell colony-enhancing factor (PBEF\/visfatin) expression and protects them from apoptosis","volume":"29","author":"CE Kendal-Wright","year":"2008","journal-title":"Placenta"},{"key":"pone.0256545.ref035","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0002-9378(95)90087-X","article-title":"Expression of cyclooxygenase types 1 and 2 in human fetal membranes at term","volume":"172","author":"DM Slater","year":"1995","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0256545.ref036","doi-asserted-by":"crossref","first-page":"e34707","DOI":"10.1371\/journal.pone.0034707","article-title":"Nuclear factor kappa B activation occurs in the amnion prior to labour onset and modulates the expression of numerous labour associated genes","volume":"7","author":"S Lim","year":"2012","journal-title":"PLoS One"},{"key":"pone.0256545.ref037","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1515\/JPM.2008.034","article-title":"Exodus-1 (CCL20): evidence for the participation of this chemokine in spontaneous labor at term, preterm labor, and intrauterine infection","volume":"36","author":"N Hamill","year":"2008","journal-title":"J Perinat Med"},{"key":"pone.0256545.ref038","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1016\/S0002-9378(96)70221-1","article-title":"Inflammatory cytokine (interleukins 1, 6 and 8 and tumor necrosis factor-alpha) release from cultured human fetal membranes in response to endotoxic lipopolysaccharide mirrors amniotic fluid concentrations","volume":"174","author":"SJ Fortunato","year":"1996","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0256545.ref039","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1034\/j.1600-0897.2001.d01-11.x","article-title":"IL-1beta and IL-8 in human fetal membranes: changes with gestational age, labor, and culture conditions","volume":"46","author":"CL Elliott","year":"2001","journal-title":"Am J Reprod Immunol"},{"key":"pone.0256545.ref040","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/0002-9378(95)90562-6","article-title":"Expression of inflammatory cytokines (interleukin-1 beta and interleukin-6) in amniochorionic membranes","volume":"172","author":"R Menon","year":"1995","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0256545.ref041","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1111\/j.1600-0897.1994.tb01113.x","article-title":"Expression of TNF-alpha and TNFR p55 in cultured amniochorion","volume":"32","author":"SJ Fortunato","year":"1994","journal-title":"Am J Reprod Immunol"},{"key":"pone.0256545.ref042","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1165\/rcmb.2002-0272OC","article-title":"CCL20 is an inducible product of human airway epithelia with innate immune properties","volume":"29","author":"TD Starner","year":"2003","journal-title":"Am J Respir Cell Mol Biol"},{"key":"pone.0256545.ref043","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1111\/j.1471-0528.2002.01045.x","article-title":"Does endothelial cell activation occur with intrauterine growth restriction?","volume":"109","author":"MR Johnson","year":"2002","journal-title":"BJOG"},{"key":"pone.0256545.ref044","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1007\/s00418-014-1304-0","article-title":"Placental fractalkine mediates adhesion of THP-1 monocytes to villous trophoblast","volume":"143","author":"M Siwetz","year":"2015","journal-title":"Histochem Cell Biol"},{"key":"pone.0256545.ref045","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1186\/1476-9255-11-12","article-title":"Chorioamnionitis (ChA) modifies CX3CL1 (fractalkine) production by human amniotic epithelial cells (HAEC) under normoxic and hypoxic conditions","volume":"11","author":"D Szukiewicz","year":"2014","journal-title":"J Inflamm"},{"key":"pone.0256545.ref046","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/j.ajpath.2015.01.019","article-title":"Placental fractalkine is up-regulated in severe early-onset preeclampsia","volume":"185","author":"M Siwetz","year":"2015","journal-title":"Am J Pathol"},{"key":"pone.0256545.ref047","first-page":"44","article-title":"Leucocyte density and pro-inflammatory cytokine expression in human fetal membranes, decidua, cervix and myometrium before and during term labour","volume":"9","author":"AY Inass Osman","year":"2003","journal-title":"Mol Hum Reprod"},{"key":"pone.0256545.ref048","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1006\/dbio.1998.9122","article-title":"IL-10 is an autocrine inhibitor of human placental cytotrophoblast MMP-9 production and invasion","volume":"205","author":"I Roth","year":"1999","journal-title":"Dev Biol"},{"key":"pone.0256545.ref049","first-page":"514","article-title":"Endocrine and Paracrine Regulation of Birth at Term and Preterm","volume":"21","author":"JRG Challis","year":"2000","journal-title":"Endocr Rev"},{"key":"pone.0256545.ref050","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1016\/S0002-9378(99)70400-X","article-title":"Cytokine abundance in placental tissues: evidence of inflammatory activation in gestational membranes with term and preterm parturition","volume":"181","author":"JA Keelan","year":"1999","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0256545.ref051","doi-asserted-by":"crossref","first-page":"4332","DOI":"10.1210\/jcem.83.12.5335","article-title":"Labor-associated changes in interleukin-10 production and its regulation by immunomodulators in human choriodecidua","volume":"83","author":"KL Simpson","year":"1998","journal-title":"J Clin Endocrinol Metab"},{"key":"pone.0256545.ref052","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jri.2009.08.002","article-title":"Placental blood leukocytes are functional and phenotypically different than peripheral leukocytes during human labor","volume":"84","author":"R Vega-Sanchez","year":"2010","journal-title":"J Reprod Immunol"},{"key":"pone.0256545.ref053","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1095\/biolreprod66.2.445","article-title":"Immunolocalization of proinflammatory cytokines in myometrium, cervix, and fetal membranes during human parturition at term","volume":"66","author":"A Young","year":"2002","journal-title":"Biol Reprod"},{"key":"pone.0256545.ref054","first-page":"404","article-title":"Inflammatory mediators regulate interleukin-8 production by cultured gestational tissues: evidence for a cytokine network at the chorio-decidual interface","volume":"76","author":"DJ Dudley","year":"1993","journal-title":"J Clin Endocrinol Metab"},{"key":"pone.0256545.ref055","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1095\/biolreprod48.1.33","article-title":"A murine model of preterm labor: inflammatory mediators regulate the production of prostaglandin E2 and interleukin-6 by murine decidua","volume":"48","author":"DJ Dudley","year":"1993","journal-title":"Biol Reprod"},{"key":"pone.0256545.ref056","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1016\/0002-9378(92)91619-L","article-title":"Origin of cervical collagenase during parturition","volume":"166","author":"R Osmers","year":"1992","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0256545.ref057","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1177\/1933719115585148","article-title":"Expression of Interferon gamma by Decidual Cells and Natural Killer Cells at the Human Implantation Site: Implications for Preeclampsia, Spontaneous Abortion, and Intrauterine Growth Restriction","volume":"22","author":"CP Chen","year":"2015","journal-title":"Reprod Sci"},{"key":"pone.0256545.ref058","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.ajpath.2013.05.029","article-title":"Decidual cell regulation of natural killer cell-recruiting chemokines: implications for the pathogenesis and prediction of preeclampsia","volume":"183","author":"CJ Lockwood","year":"2013","journal-title":"Am J Pathol"},{"key":"pone.0256545.ref059","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1189\/jlb.71.2.173","article-title":"Role of chemokines in the biology of natural killer cells","volume":"71","author":"MJ Robertson","year":"2002","journal-title":"J Leukoc Biol"},{"key":"pone.0256545.ref060","doi-asserted-by":"crossref","first-page":"7833","DOI":"10.4049\/jimmunol.177.11.7833","article-title":"Evidence for NK cell subsets based on chemokine receptor expression","volume":"177","author":"RD Berahovich","year":"2006","journal-title":"J Immunol"},{"key":"pone.0256545.ref061","article-title":"Tethering IL2 to its receptor IL2Rbeta enhances anti-tumor activity and expansion of natural killer NK92 cells","author":"Y Jounaidi","year":"2017","journal-title":"Cancer Res"},{"key":"pone.0256545.ref062","doi-asserted-by":"crossref","first-page":"3966","DOI":"10.1016\/j.febslet.2009.10.036","article-title":"Correlation of mRNA and protein in complex biological samples","volume":"583","author":"T Maier","year":"2009","journal-title":"FEBS Lett"},{"key":"pone.0256545.ref063","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1677\/joe.0.1610143","article-title":"Oxytocin receptor expression in human term and preterm gestational tissues prior to and following the onset of labour","volume":"161","author":"D Wathes","year":"1999","journal-title":"Journal of Endocrinology"},{"key":"pone.0256545.ref064","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1507\/endocrj1954.31.565","article-title":"Study of oxytocin receptor: II. oxytocin and prostaglandin F2 alpha receptors in human myometria and amnion-decidua complex during pregnancy and labor","volume":"31","author":"H Fukai","year":"1984","journal-title":"Endocrinol Jpn"},{"key":"pone.0256545.ref065","first-page":"3392","article-title":"Paracrine Oxytocin and Estradiol Demonstrate a Spatial Increase in Human Intrauterine Tissues with Labor. 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