{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:42:40Z","timestamp":1789684960494,"version":"4.0.1"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programa Jovens Pesquisadores"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Wild animals infected with\n                    <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n                    represent important indicators of this fungal agent presence in the environment. The detection of this pathogen in road-killed wild animals has shown to be a key strategy for eco-epidemiological surveillance of paracoccidioidomycosis (PCM), helping to map hot spots for human infection. Molecular detection of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    in wild animals from PCM outbreak areas has not been performed so far. The authors investigated the presence of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    through nested-PCR in tissue samples obtained from road-killed animals collected nearby a human PCM outbreak spot, Rio de Janeiro state, Brazil and border areas. Eighteen species of mammals were analyzed:\n                    <jats:italic>Dasypus novemcinctus<\/jats:italic>\n                    (nine-banded armadillo, n = 6),\n                    <jats:italic>Cerdocyon thous<\/jats:italic>\n                    (crab-eating fox, n = 4),\n                    <jats:italic>Coendou spinosus<\/jats:italic>\n                    (hairy dwarf porcupine, n = 2),\n                    <jats:italic>Lontra longicaudis<\/jats:italic>\n                    (Neotropical river otter, n = 1),\n                    <jats:italic>Procyon cancrivorus<\/jats:italic>\n                    (crab-eating raccoon, n = 1),\n                    <jats:italic>Galactis cuja<\/jats:italic>\n                    (lesser grison, n = 1),\n                    <jats:italic>Tamandua tetradactyla<\/jats:italic>\n                    (collared anteater, n = 1),\n                    <jats:italic>Cuniculus paca<\/jats:italic>\n                    (paca, n = 1), and\n                    <jats:italic>Bradypus variegatus<\/jats:italic>\n                    (brown-throated three-toed sloth, n = 1). Specific\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    sequences were detected in the liver, spleen, and lymph node samples from 4\/6 (66.7%)\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>novemcinctus<\/jats:italic>\n                    , reinforcing the importance of these animals on\n                    <jats:italic>Paracoccidioides<\/jats:italic>\n                    ecology. Moreover, lymph nodes samples from two\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>thous<\/jats:italic>\n                    , as well as lung samples from the\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>paca<\/jats:italic>\n                    were also positive. A literature review of\n                    <jats:italic>Paracoccidioides<\/jats:italic>\n                    spp. in vertebrates in Brazil indicates\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>thous<\/jats:italic>\n                    and\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>paca<\/jats:italic>\n                    as new hosts for the fungal pathogen\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0256668","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T14:09:04Z","timestamp":1629814144000},"page":"e0256668","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular eco-epidemiology of Paracoccidioides brasiliensis in road-killed mammals reveals Cerdocyon thous and Cuniculus paca as new hosts harboring this fungal pathogen"],"prefix":"10.1371","volume":"16","author":[{"given":"Bruno","family":"de Souza 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