{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:57:21Z","timestamp":1791385041104,"version":"4.3.3"},"reference-count":75,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","award":["IRN FY2020 507\/2563"],"award-info":[{"award-number":["IRN FY2020 507\/2563"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"crossref","award":["CU_FRB640001_01_33_3"],"award-info":[{"award-number":["CU_FRB640001_01_33_3"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The use of endotoxin, such as lipopolysaccharide (LPS) as a model of sickness behavior, has attracted recent attention. To objectively investigate sickness behavior along with its pain-like behaviors in LPS-treated mice, the behavioral measurement requires accurate methods, which reflects clinical relevance. While reflexive pain response tests have been used for decades for pain assessment, its accuracy and clinical relevance remain problematic. Hence, we used automated home-cage monitoring LABORAS to evaluate spontaneous locomotive behaviors in LPS-induced mice. LPS-treated mice displayed sickness behaviors including pain-like behaviors in automated home-cage monitoring characterized by decreased mobile behaviors (climbing, locomotion, rearing) and increased immobility compared to that of the control group in both short- and long-term locomotive assessments. Here, in short-term measurement, both in the open-field test and automated home-cage monitoring, mice demonstrated impaired locomotive behaviors. We also assessed 24 h long-term locomotor activity in the home-cage system, which profiled the diurnal behaviors of LPS-stimulated mice. The results demonstrated significant behavioral impairment in LPS-stimulated mice compared to the control mice in both light and dark phases. However, the difference is more evident in the dark phase compared to the light phase owing to the nocturnal activity of mice. In addition, the administration of indomethacin as a pharmacological intervention improved sickness behaviors in the open-field test as well as automated home-cage monitoring, confirming that automated home-cage monitoring could be potentially useful in pharmacological screening. Together, our results demonstrate that automated home-cage monitoring could be a feasible alternative to conventional methods, such as the open-field test and combining several behavioral assessments may provide a better understanding of sickness behavior and pain-like behaviors in LPS-treated mice.<\/jats:p>","DOI":"10.1371\/journal.pone.0256706","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T17:32:05Z","timestamp":1630085525000},"page":"e0256706","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4063-3681","authenticated-orcid":true,"family":"Hasriadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3735-799X","authenticated-orcid":true,"given":"Peththa Wadu","family":"Dasuni 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analgesics","volume":"16","author":"CJ Woolf","year":"2010","journal-title":"Nat Med"},{"key":"pone.0256706.ref006","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/nbt.2786","article-title":"Clinical development success rates for investigational drugs","volume":"32","author":"M Hay","year":"2014","journal-title":"Nat Biotechnol"},{"key":"pone.0256706.ref007","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.tips.2012.08.001","article-title":"Current challenges in translational pain research","volume":"33","author":"J Mao","year":"2012","journal-title":"Trends Pharmacol Sci"},{"key":"pone.0256706.ref008","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.neubiorev.2020.05.001","article-title":"Comparison of bacterial lipopolysaccharide-induced sickness behavior in rodents and humans: Relevance for symptoms of anxiety and depression","volume":"115","author":"J Lasselin","year":"2020","journal-title":"Neurosci Biobehav Rev."},{"key":"pone.0256706.ref009","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12974-016-0539-1","article-title":"Activation of the NLRP3 inflammasome in lipopolysaccharide-induced mouse fatigue and its relevance to chronic fatigue syndrome","volume":"13","author":"Z-T Zhang","year":"2016","journal-title":"J Neuroinflammation"},{"key":"pone.0256706.ref010","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1002\/glia.22961","article-title":"Sleep and immunomodulatory responses to systemic lipopolysaccharide in mice selectively expressing interleukin-1 receptor 1 on neurons or astrocytes","volume":"64","author":"AM Ingiosi","year":"2016","journal-title":"Glia"},{"key":"pone.0256706.ref011","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/0304-3940(93)90189-R","article-title":"Lipopolysaccharide induces fever and decreases tail flick latency in awake rats","volume":"154","author":"P Mason","year":"1993","journal-title":"Neurosci 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mechanisms of endotoxemia on post-incisional pain in rats","volume":"148","author":"T Kawano","year":"2016","journal-title":"Life Sci"},{"key":"pone.0256706.ref022","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.bbi.2014.11.014","article-title":"Modality and sex differences in pain sensitivity during human endotoxemia","volume":"46","author":"B Karshikoff","year":"2015","journal-title":"Brain Behav Immun"},{"key":"pone.0256706.ref023","doi-asserted-by":"crossref","first-page":"e84159","DOI":"10.1371\/journal.pone.0084159","article-title":"Systemic Inflammation Decreases Pain Threshold in Humans In Vivo.","volume":"8","author":"M de Goeij","year":"2013","journal-title":"PLoS One"},{"key":"pone.0256706.ref024","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.bbi.2014.05.001","article-title":"Inflammation-induced hyperalgesia: Effects of timing, dosage, and negative affect on somatic pain sensitivity in human experimental endotoxemia","volume":"41","author":"A Wegner","year":"2014","journal-title":"Brain Behav Immun"},{"key":"pone.0256706.ref025","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.bbi.2013.03.002","article-title":"Low-dose endotoxin potentiates capsaicin-induced pain in man: Evidence for a pain neuroimmune connection","volume":"30","author":"MR Hutchinson","year":"2013","journal-title":"Brain Behav Immun"},{"key":"pone.0256706.ref026","first-page":"60","article-title":"Acute Inflammatory Response to Endotoxin in Mice and Humans","volume":"12","author":"S Copeland","year":"2005","journal-title":"Clin Diagn Lab Immunol"},{"key":"pone.0256706.ref027","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/j.bbi.2011.06.006","article-title":"Cytokine and chemokine responses in serum and brain after single and repeated injections of lipopolysaccharide: multiplex quantification with path analysis.","volume":"25","author":"MA Erickson","year":"2011","journal-title":"Brain 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Jiang","year":"2013","journal-title":"PLoS One"},{"key":"pone.0256706.ref031","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/nrn1700","article-title":"Role of the Immune system in chronic pain","volume":"6","author":"F Marchand","year":"2005","journal-title":"Nat Rev Neurosci"},{"key":"pone.0256706.ref032","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1177\/0022034511400225","article-title":"LPS sensitizes TRPV1 via activation of TLR4 in trigeminal sensory neurons","volume":"90","author":"A Diogenes","year":"2011","journal-title":"J Dent Res"},{"key":"pone.0256706.ref033","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1038\/ncomms4125","article-title":"TRPA1 channels mediate acute neurogenic inflammation and pain produced by bacterial endotoxins","volume":"5","author":"V Meseguer","year":"2014","journal-title":"Nat 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Immun"},{"key":"pone.0256706.ref037","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.bbi.2020.04.033","article-title":"Challenging the challenge: A randomized controlled trial evaluating the inflammatory response and pain perception of healthy volunteers after single-dose LPS administration, as a potential model for inflammatory pain in early-phase drug development","volume":"88","author":"HJ Hijma","year":"2020","journal-title":"Brain Behav Immun"},{"key":"pone.0256706.ref038","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.pain.2004.09.028","article-title":"What should we be measuring in behavioral studies of chronic pain in animals?","volume":"112","author":"JS Mogil","year":"2004","journal-title":"Pain"},{"key":"pone.0256706.ref039","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/nrn2606","article-title":"Animal models of pain: progress and challenges","volume":"10","author":"JS Mogil","year":"2009","journal-title":"Nat Rev Neurosci"},{"key":"pone.0256706.ref040","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.neubiorev.2019.03.009","article-title":"Pros and Cons of Clinically Relevant Methods to Assess Pain in Rodents.","volume":"100","author":"A Tappe-Theodor","year":"2019","journal-title":"Neurosci Biobehav Rev"},{"key":"pone.0256706.ref041","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.jpain.2004.09.001","article-title":"Neuropathic pain symptoms relative to overall pain rating","volume":"5","author":"M-M Backonja","year":"2004","journal-title":"J Pain"},{"key":"pone.0256706.ref042","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.physbeh.2010.01.038","article-title":"Housing environment influences the need for pain relief during post-operative recovery in mice","volume":"99","author":"TM Pham","year":"2010","journal-title":"Physiol Behav"},{"key":"pone.0256706.ref043","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nmeth.2935","article-title":"Olfactory exposure to males, including men, causes stress and related analgesia in rodents","volume":"11","author":"RE Sorge","year":"2014","journal-title":"Nat Methods"},{"key":"pone.0256706.ref044","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1097\/00000542-200404000-00003","article-title":"Did experimenter bias conceal the efficacy of spinal opioids in previous studies with the spinal nerve ligation model of neuropathic pain?","author":"JC Eisenach","year":"2004","journal-title":"Anesthesiology"},{"key":"pone.0256706.ref045","article-title":"The Measurement of Pain in the Laboratory Rodent.","author":"JS Mogil","year":"2018","journal-title":"The Oxford Handbook of the Neurobiology of Pain"},{"key":"pone.0256706.ref046","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.neubiorev.2020.03.004","article-title":"The search for translational pain outcomes to refine analgesic development: Where did we come from and where are we going?","volume":"113","author":"R Gonz\u00e1lez-Cano","year":"2020","journal-title":"Neurosci Biobehav Rev"},{"key":"pone.0256706.ref047","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1002\/j.1532-2149.2011.00099.x","article-title":"Assessing carrageenan-induced locomotor activity impairment in rats: Comparison with evoked endpoint of acute inflammatory pain","volume":"16","author":"CZ Zhu","year":"2012","journal-title":"Eur J Pain"},{"key":"pone.0256706.ref048","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1002\/ejp.686","article-title":"Complete Freund\u2019s adjuvant-induced reduction of exploratory activity in a novel environment as an objective nociceptive endpoint for sub-acute inflammatory pain model in rats","volume":"19","author":"CZ Zhu","year":"2015","journal-title":"Eur J Pain"},{"key":"pone.0256706.ref049","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1089\/08977150152693773","article-title":"Changes in exploratory behavior as a measure of chronic central pain following spinal cord injury","volume":"18","author":"CD Mills","year":"2001","journal-title":"J Neurotrauma"},{"key":"pone.0256706.ref050","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1097\/00000542-200407000-00030","article-title":"Effects of laparotomy on spontaneous exploratory activity and conditioned operant responding in the rat: a model for postoperative pain","volume":"101","author":"TJ Martin","year":"2004","journal-title":"Anesthesiology"},{"key":"pone.0256706.ref051","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.bbi.2009.11.006","article-title":"The effect of non-steroidal anti-inflammatory agents on behavioural changes and cytokine production following systemic inflammation: Implications for a role of COX-1","volume":"24","author":"JL Teeling","year":"2010","journal-title":"Brain Behav Immun"},{"key":"pone.0256706.ref052","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.ddtec.2006.06.010","article-title":"Advanced behavioural screening: automated home cage ethology.","volume":"3","author":"BM Spruijt","year":"2006","journal-title":"Drug Discov Today Technol"},{"key":"pone.0256706.ref053","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.neuroscience.2012.06.024","article-title":"Minocycline blocks lipopolysaccharide induced hyperalgesia by suppression of microglia but not astrocytes","volume":"221","author":"S-Y Yoon","year":"2012","journal-title":"Neuroscience"},{"key":"pone.0256706.ref054","first-page":"162","article-title":"Antinociceptive effect of pregabalin in septic shock-induced rectal hypersensitivity in rats","volume":"295","author":"H Eutamene","year":"2000","journal-title":"J Pharmacol Exp Ther"},{"key":"pone.0256706.ref055","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.pneurobio.2009.04.003","article-title":"Stress-induced analgesia","volume":"88","author":"RK Butler","year":"2009","journal-title":"Prog Neurobiol"},{"key":"pone.0256706.ref056","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.2741\/1960","article-title":"Stress-induced hyperalgesia: animal models and putative mechanisms","volume":"11","author":"H Imbe","year":"2006","journal-title":"Front Biosci"},{"key":"pone.0256706.ref057","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.biopsych.2008.07.024","article-title":"Systemic inflammation induces acute behavioral and cognitive changes and accelerates neurodegenerative disease","volume":"65","author":"C Cunningham","year":"2009","journal-title":"Biol Psychiatry"},{"key":"pone.0256706.ref058","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.brainresbull.2017.09.010","article-title":"Proanthocyanidin prevents lipopolysaccharide-induced depressive-like behavior in mice via neuroinflammatory pathway","volume":"135","author":"X Jiang","year":"2017","journal-title":"Brain Res Bull"},{"key":"pone.0256706.ref059","first-page":"18","article-title":"Lipopolysaccharide-induced depressive-like behavior is associated with \u03b1\u2081-adrenoceptor dependent downregulation of the membrane GluR1 subunit in the mouse medial prefrontal cortex and ventral tegmental area.","author":"M Sekio","year":"2014","journal-title":"Int J Neuropsychopharmacol"},{"key":"pone.0256706.ref060","first-page":"12","article-title":"Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions","author":"C Pitzer","year":"2016","journal-title":"Mol Pain"},{"key":"pone.0256706.ref061","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.applanim.2011.02.007","article-title":"A comparison of a manual and an automated behavioural analysis method for assessing post-operative pain in mice","volume":"131","author":"AL Miller","year":"2011","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0256706.ref062","doi-asserted-by":"crossref","first-page":"e564","DOI":"10.1097\/PR9.0000000000000564","article-title":"Voluntary and evoked behavioral correlates in inflammatory pain conditions under different social housing conditions","volume":"1","author":"C Pitzer","year":"2016","journal-title":"PAIN Reports"},{"key":"pone.0256706.ref063","doi-asserted-by":"crossref","first-page":"4015","DOI":"10.1002\/art.23063","article-title":"Collagen-induced arthritis as a model of hyperalgesia: Functional and cellular analysis of the analgesic actions of tumor necrosis factor blockade","volume":"56","author":"JJ Inglis","year":"2007","journal-title":"Arthritis Rheum"},{"key":"pone.0256706.ref064","doi-asserted-by":"crossref","first-page":"100101","DOI":"10.1016\/j.ocarto.2020.100101","article-title":"Comparison of LABORAS with static incapacitance testing for assessing spontaneous pain behaviour in surgically-induced murine osteoarthritis","author":"IS von Loga","year":"2020","journal-title":"Osteoarthr Cartil Open"},{"key":"pone.0256706.ref065","doi-asserted-by":"crossref","first-page":"3110","DOI":"10.1002\/art.23870","article-title":"Regulation of pain sensitivity in experimental osteoarthritis by the endogenous peripheral opioid system","volume":"58","author":"JJ Inglis","year":"2008","journal-title":"Arthritis Rheum"},{"key":"pone.0256706.ref066","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1038\/s41598-018-19901-1","article-title":"A unique inbred rat strain with sustained cephalic hypersensitivity as a model of chronic migraine-like pain","volume":"8","author":"G Munro","year":"2018","journal-title":"Sci Rep"},{"key":"pone.0256706.ref067","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/S0531-5565(00)00128-5","article-title":"Interaction of indomethacin with cytokine production in whole blood. Potential mechanism for a brain-protective effect","volume":"35","author":"AM Bour","year":"2000","journal-title":"Exp Gerontol"},{"key":"pone.0256706.ref068","first-page":"47","article-title":"Indomethacin and cyclosporin a inhibit in vitro ischemia-induced expression of ICAM-1 and chemokines in human brain endothelial cells","volume":"76","author":"W Zhang","year":"2000","journal-title":"Acta Neurochir Suppl"},{"key":"pone.0256706.ref069","doi-asserted-by":"crossref","first-page":"1710 LP","DOI":"10.1136\/annrheumdis-2013-203416","article-title":"Augmented pain behavioural responses to intra-articular injection of nerve growth factor in two animal models of osteoarthritis","volume":"73","author":"S Ashraf","year":"2014","journal-title":"Ann Rheum Dis"},{"key":"pone.0256706.ref070","doi-asserted-by":"crossref","first-page":"e2005326","DOI":"10.1371\/journal.pbio.2005326","article-title":"NGL-3 in the regulation of brain development, Akt\/GSK3b signaling, long-term depression, and locomotive and cognitive behaviors","volume":"17","author":"H Lee","year":"2019","journal-title":"PLoS Biol"},{"key":"pone.0256706.ref071","doi-asserted-by":"crossref","first-page":"e104150","DOI":"10.15252\/embj.2019104150","article-title":"Splice-dependent trans-synaptic PTP\u03b4-IL1RAPL1 interaction regulates synapse formation and non-REM sleep","volume":"39","author":"H Park","year":"2020","journal-title":"EMBO J"},{"key":"pone.0256706.ref072","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/j.1440-1681.2012.05719.x","article-title":"Rodent motor and neuropsychological behaviour measured in home cages using the integrated modular platform SmartCageTM","volume":"39","author":"V Khroyan T","year":"2012","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"pone.0256706.ref073","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1254\/jphs.FPJ04039X","article-title":"Combined effects of psychostimulants and morphine on locomotor activity in mice.","volume":"96","author":"T Mori","year":"2004","journal-title":"J Pharmacol Sci"},{"key":"pone.0256706.ref074","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.seizure.2017.09.015","article-title":"Behavioral effects of antiepileptic drugs in rats: Are the effects on mood and behavior detectable in open-field test?","volume":"52","author":"E Zimcikova","year":"2017","journal-title":"Seizure"},{"key":"pone.0256706.ref075","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1097\/j.pain.0000000000000256","article-title":"Inflammation-induced pain sensitization in men and women: does sex matter in experimental endotoxemia?","volume":"156","author":"A Wegner","year":"2015","journal-title":"Pain"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0256706","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T17:32:40Z","timestamp":1630085560000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0256706"}},"subtitle":[],"editor":[{"given":"David D.","family":"McKemy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,8,27]]},"references-count":75,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0256706","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,27]]}}}