{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T01:16:27Z","timestamp":1788743787519,"version":"build-2803163510"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004610","name":"science and technology support program of jiangsu province","doi-asserted-by":"publisher","award":["2020YJ0352"],"award-info":[{"award-number":["2020YJ0352"]}],"id":[{"id":"10.13039\/501100004610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"sichuan province science and technology support program","doi-asserted-by":"publisher","award":["2020YJ0411"],"award-info":[{"award-number":["2020YJ0411"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Rice blast, caused by the fungus\n                    <jats:italic>Magnaporthe oryzae<\/jats:italic>\n                    , is one of the three major diseases affecting rice production and quality; it reduces rice grain yield by nearly 30%. In the early stage of this study, a strain of\n                    <jats:italic>Bacillus velezensis<\/jats:italic>\n                    with strong inhibition of\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>oryzae<\/jats:italic>\n                    was isolated and named ZW10.\n                    <jats:italic>In vitro<\/jats:italic>\n                    assays indicated prolonged germination time of conidia of\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>oryzae<\/jats:italic>\n                    treated with the antifungal substances of ZW10, 78% of the conidia could not form appressorium, and the conidial tubes expanded to form vacuolar structure and then shrank. The results of FDA-PI composite dyes showed that the antifungal substances of ZW10 inhibited the normal activity of\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>oryzae<\/jats:italic>\n                    hyphae that were rarely able to infect the epidermal cells of rice leaf sheath\n                    <jats:italic>in vivo<\/jats:italic>\n                    tests. In addition, rice treated with the antifungal substances of ZW10 showed a variety of defense responses, including activation of defense-related enzymes, increased expression of the salicylic acid pathway genes, and accumulation of hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ), which might function directly or indirectly in resistance to pathogen attack. The field experiment with rice blast infection in different periods showed that the antifungal substances of ZW10 had the same control effect as carbendazim. The significant biological control activity of ZW10 and its capacity to stimulate host defenses suggest that this\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>velezensis<\/jats:italic>\n                    strain has the potential to be developed into a biopesticide for the biocontrol of rice blast.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0256807","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T13:39:00Z","timestamp":1630071540000},"page":"e0256807","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["The antagonistic mechanism of Bacillus velezensis ZW10 against rice blast disease: Evaluation of ZW10 as a potential 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overview of global rice production, supply, trade, and consumption","volume":"1324","author":"S Muthayya","year":"2014","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"1","key":"pone.0256807.ref002","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1146\/annurev.micro.57.030502.090957","article-title":"On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea","volume":"57","author":"NJ Talbot","year":"2003","journal-title":"Annual Review of Microbiology"},{"issue":"7036","key":"pone.0256807.ref003","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1038\/nature03449","article-title":"The genome sequence of the rice blast fungus Magnaporthe grisea","volume":"434","author":"RA Dean","year":"2005","journal-title":"Nature"},{"issue":"1","key":"pone.0256807.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-016-2690-6","article-title":"Host specialization of the blast fungus Magnaporthe oryzae is associated 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Mechanisms of nutrient acquisition and utilization during infection by the rice blast fungus Magnaporthe oryzae","volume":"25","author":"J Fernandez","year":"2012","journal-title":"Mol Plant Microbe Interact"},{"issue":"6","key":"pone.0256807.ref032","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1442-2042.2004.00803.x","article-title":"Reversal of acquired cisplatin resistance by modulation of metallothionein in transplanted murine tumors. 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Ou","year":"1980","journal-title":"Annrevphytopathol"},{"key":"pone.0256807.ref036","doi-asserted-by":"crossref","first-page":"104181","DOI":"10.1016\/j.biocontrol.2019.104181","article-title":"Black pepper (Piper nigrum L.) associated endophytic Pseudomonas putida BP25 alters root phenotype and induces defense in rice (Oryza sativa L.) against blast disease incited by Magnaporthe oryzae","volume":"143","author":"M Ashajyothi","year":"2020","journal-title":"Biological Control"},{"key":"pone.0256807.ref037","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1023\/A:1020501420831","article-title":"Antibiotic production by bacterial biocontrol agents","volume":"81","author":"JM Raaijmakers","year":"2002","journal-title":"Antonie van Leeuwenhoek"},{"key":"pone.0256807.ref038","doi-asserted-by":"crossref","first-page":"104153","DOI":"10.1016\/j.biocontrol.2019.104153","article-title":"Formulations of Pseudomonas fluorescens and Burkholderia pyrrocinia control rice blast of upland rice cultivated under no-tillage system","volume":"144","author":"Chaibub AA Oliveira MIdS","year":"2020","journal-title":"Biological Control"},{"issue":"5","key":"pone.0256807.ref039","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1002\/jobm.201600604","article-title":"The use of newly isolated Streptomyces strain TN258 as potential biocontrol agent of potato tubers leak caused by Pythium ultimum","volume":"57","author":"I Sellem","year":"2017","journal-title":"Journal of Basic Microbiology"},{"key":"pone.0256807.ref040","article-title":"Biocontrol potential of antagonist Bacillus subtilis Tpb55 against tobacco black shank","author":"T Han","year":"2016","journal-title":"BioControl"},{"key":"pone.0256807.ref041","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.fm.2016.11.004","article-title":"Potential of a new strain of Bacillus amyloliquefaciens BUZ-14 as a biocontrol agent of postharvest fruit diseases","volume":"63","author":"H Calvo","year":"2017","journal-title":"Food Microbiology"},{"issue":"1","key":"pone.0256807.ref042","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1146\/annurev.phyto.45.062806.094346","article-title":"Magnaporthe as a Model for Understanding Host-Pathogen Interactions","volume":"45","author":"Ebbole","year":"2007","journal-title":"Annual Review of Phytopathology"},{"key":"pone.0256807.ref043","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.pestbp.2019.09.003","article-title":"Antifungal activity of endophytic Bacillus safensis B21 and its potential application as a biopesticide to control rice blast","volume":"162","author":"S Rong","year":"2020","journal-title":"Pesticide Biochemistry and Physiology"},{"issue":"50","key":"pone.0256807.ref044","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1146\/annurev.micro.50.1.491","article-title":"Breaking and entering: host penetration by the fungal rice blast pathogen magnaporthe 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