{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:26:04Z","timestamp":1790738764269,"version":"4.1.0"},"reference-count":104,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012964","name":"Minist\u00e8re de l\u2019Enseignement Sup\u00e9rieur et de la Recherche Scientifique","doi-asserted-by":"crossref","award":["PRIMA\/INTOMED"],"award-info":[{"award-number":["PRIMA\/INTOMED"]}],"id":[{"id":"10.13039\/100012964","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012958","name":"Minist\u00e8re de l'Education Nationale, de la Formation professionnelle, de l'Enseignement Sup\u00e9rieur et de la Recherche Scientifique","doi-asserted-by":"crossref","award":["PRF2019-D6P2"],"award-info":[{"award-number":["PRF2019-D6P2"]}],"id":[{"id":"10.13039\/100012958","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    <jats:italic>Ceratitis capitata<\/jats:italic>\n                    (medfly) is one of the most devastating crop pests worldwide. The Sterile Insect Technique (SIT) is a control method that is based on the mass rearing of males, their sterilization, and release in the field. However, the effectiveness of the technique depends on the quality of the released males and their fitness. We previously isolated and selected a probiotic bacteria (\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    sp.), from wild-caught medflies, according to criteria that improved biological quality traits of reared medfly males.We firstly evaluated the impact of the irradiation on the expression of different immune and stress genes in the medfly sterile males. Expression was measured at differents time points ranging from 0 to 168 h after irradiation to capture the response of genes with distinct temporal expression patterns. Then, we supplemented the larval diet with previously isolated\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    sp.strain, live and autoclaved at various concentrations to see whether the probiotic treatments affect, through their protective role, the gene expression level, and quality traits. The irradiation had significant effect on the genes\n                    <jats:italic>attacin<\/jats:italic>\n                    ,\n                    <jats:italic>cecropin<\/jats:italic>\n                    ,\n                    <jats:italic>PGPR-LC<\/jats:italic>\n                    ,\n                    <jats:italic>hsp23<\/jats:italic>\n                    , and\n                    <jats:italic>hsp70<\/jats:italic>\n                    level expression. The expression of\n                    <jats:italic>attacin<\/jats:italic>\n                    and\n                    <jats:italic>PGPR-LC<\/jats:italic>\n                    was up-regulated while that of\n                    <jats:italic>cecropin<\/jats:italic>\n                    was down-regulated.\n                    <jats:italic>Hsp<\/jats:italic>\n                    genes showed decreased levels between 0 and 18 h to peak at 72 h. However, the supplementation of the probiotic strain, either live or autoclaved, was statistically significant only for\n                    <jats:italic>attacin<\/jats:italic>\n                    gene. However, significant interaction time x probiotic was noticed for\n                    <jats:italic>attacin<\/jats:italic>\n                    ,\n                    <jats:italic>cecropin<\/jats:italic>\n                    ,\n                    <jats:italic>hsp23<\/jats:italic>\n                    and\n                    <jats:italic>hsp70<\/jats:italic>\n                    . The probiotic treatments also improved the quality control parameters like pupal weight. From this work we can conclude that a consortium of parabiotics (autoclaved probiotics) treatment will be recommended in insectaries considering both the beneficial effects on mass reared insects and its general safety for insectary workers and for environment.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0257097","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T13:43:54Z","timestamp":1631281434000},"page":"e0257097","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9662-1116","authenticated-orcid":true,"given":"Meriem","family":"Msaad Guerfali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamel","family":"Charaabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haytham","family":"Hamden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wafa","family":"Djobbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma","family":"Fadhl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amor","family":"Mosbah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameur","family":"Cherif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,10]]},"reference":[{"key":"pone.0257097.ref001","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1093\/jee\/83.5.1863","article-title":"Host plants of Mediterranean fruit fly (Diptera: Tephritidae) on the island of Hawaii (1949\u20131985)","volume":"83","author":"N.J. 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