{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T02:59:06Z","timestamp":1785812346336,"version":"3.56.0"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"The Second Century Fund (C2F), Chulalongkorn University"},{"name":"The Jongkolneenithi foundation"},{"name":"The Medical Association of Thailand"},{"name":"The Tropical Medicine Cluster, Chulalongkorn University"},{"name":"Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>The dengue vaccine (Dengvaxia) is only recommended for individuals with prior dengue infection (PDI). This study aimed to perform a serosurvey to inform decision-making for vaccine introduction and identify appropriate target populations. We also evaluated the performance of the serological tests using plaque reduction neutralization test (PRNT) as a reference test in identifying PDI to determine suitability for pre-vaccination screening.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methods<\/jats:title><jats:p>We enrolled 115 healthy individuals between 10 and 22 years of age living in the Ratchaburi province of Thailand. The serum samples were tested by PRNT to measure the prevalence and concentration of serotype-specific neutralizing antibodies. The performance of the IgG rapid diagnostic test (RDT, SD Bioline, Korea) and IgG enzyme-linked immunosorbent assay (ELISA, EUROIMMUN, Germany) in identifying PDI were evaluated by using PRNT as a reference method.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Results<\/jats:title><jats:p>Ninety-four (81.7%) individuals neutralized one or more dengue serotypes at a titer threshold greater than or equal to 10. Multitypic profiles were observed in 70.4% of the samples which increased to 91.9% in subjects aged 19\u201322. Among monotypic samples, the highest proportion was reactive against DENV-1 followed by DENV-2, DENV-3, and DENV-4. The highest anti-dengue antibody titers were recorded against DENV-1 and increased with age to a geometric mean NT50 titer (GMT) of 188.6 in the 19\u201322 age group. While both RDT and ELISA exhibited 100% specificity, RDT demonstrated low sensitivity (35%) with ELISA displaying much greater sensitivity (87%).<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Conclusions<\/jats:title><jats:p>Almost 80% of adolescents and youth in Ratchaburi province had already been exposed to one or more of the dengue virus serotypes. The dengue IgG RDT displayed low sensitivity and is likely not be suitable for dengue pre-vaccination screening. These results support the use of IgG ELISA test for dengue vaccination in endemic areas.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pone.0257182","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T22:07:20Z","timestamp":1631311640000},"page":"e0257182","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1521-5592","authenticated-orcid":true,"given":"Umaporn","family":"Limothai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasipha","family":"Tachaboon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janejira","family":"Dinhuzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taweewun","family":"Hunsawong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prapapun","family":"Ong-ajchaowlerd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Butsaya","family":"Thaisomboonsuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Fernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supachoke","family":"Trongkamolchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mananya","family":"Wanpaisitkul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chatchai","family":"Chulapornsiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anongrat","family":"Tiawilai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thawat","family":"Tiawilai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terapong","family":"Tantawichien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usa","family":"Thisyakorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8544-8132","authenticated-orcid":true,"given":"Nattachai","family":"Srisawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,10]]},"reference":[{"issue":"Pt 9","key":"pone.0257182.ref001","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.1099\/0022-1317-78-9-2279","article-title":"Molecular evolution and phylogeny of dengue-4 viruses","volume":"78","author":"RS Lanciotti","year":"1997","journal-title":"J Gen Virol"},{"issue":"9132","key":"pone.0257182.ref002","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/S0140-6736(97)12483-7","article-title":"Dengue and dengue haemorrhagic fever","volume":"352","author":"JG Rigau-P\u00e9rez","year":"1998","journal-title":"Lancet"},{"issue":"13","key":"pone.0257182.ref003","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1056\/NEJMoa1506223","article-title":"Efficacy and Long-Term Safety of a Dengue Vaccine in Regions of Endemic Disease","volume":"373","author":"SR Hadinegoro","year":"2015","journal-title":"N Engl J Med"},{"issue":"12","key":"pone.0257182.ref004","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1093\/infdis\/jiw341","article-title":"Population Perspectives and World Health Organization Recommendations for CYD-TDV Dengue Vaccine","volume":"214","author":"A Wilder-Smith","year":"2016","journal-title":"J Infect Dis"},{"issue":"4","key":"pone.0257182.ref005","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1056\/NEJMoa1800820","article-title":"Effect of Dengue Serostatus on Dengue Vaccine Safety and Efficacy","volume":"379","author":"S Sridhar","year":"2018","journal-title":"N Engl J Med"},{"issue":"9","key":"pone.0257182.ref006","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/j.vaccine.2016.10.070","article-title":"Dengue vaccine: WHO position paper, July 2016\u2014recommendations","volume":"35","author":"World Health Organization","year":"2017","journal-title":"Vaccine"},{"issue":"35","key":"pone.0257182.ref007","doi-asserted-by":"crossref","first-page":"4848","DOI":"10.1016\/j.vaccine.2018.09.063","article-title":"Dengue vaccine: WHO position paper, September 2018\u2014Recommendations","volume":"37","author":"World Health Organization","year":"2019","journal-title":"Vaccine"},{"issue":"4","key":"pone.0257182.ref008","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1089\/vim.2007.0050","article-title":"Antibody to dengue 1 detected more than 60 years after infection","volume":"20","author":"A Imrie","year":"2007","journal-title":"Viral Immunol"},{"issue":"2","key":"pone.0257182.ref009","doi-asserted-by":"crossref","first-page":"285","DOI":"10.4049\/jimmunol.99.2.285","article-title":"A plaque reduction test for dengue virus neutralizing antibodies","volume":"99","author":"PK Russell","year":"1967","journal-title":"J Immunol"},{"issue":"2","key":"pone.0257182.ref010","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1089\/vim.2008.0007","article-title":"Guidelines for Plaque-Reduction Neutralization Testing of Human Antibodies to Dengue Viruses","volume":"21","author":"JT Roehrig","year":"2008","journal-title":"Viral Immunol"},{"issue":"11","key":"pone.0257182.ref011","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/trstmh\/trz068","article-title":"A review of dengue diagnostics and implications for surveillance and control","volume":"113","author":"N Raafat","year":"2019","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"12 Suppl","key":"pone.0257182.ref012","doi-asserted-by":"crossref","first-page":"S30","DOI":"10.1038\/nrmicro2459","article-title":"Evaluation of diagnostic tests: dengue","volume":"8","author":"RW Peeling","year":"2010","journal-title":"Nat Rev Microbiol"},{"issue":"6","key":"pone.0257182.ref013","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.cmi.2019.01.002","article-title":"Rapid diagnostic tests for determining dengue serostatus: a systematic review and key informant interviews","volume":"25","author":"R Luo","year":"2019","journal-title":"Clin Microbiol Infect"},{"issue":"6","key":"pone.0257182.ref014","doi-asserted-by":"crossref","first-page":"519","DOI":"10.4103\/0301-4738.71699","article-title":"A simple nomogram for sample size for estimating sensitivity and specificity of medical tests","volume":"58","author":"RK Malhotra","year":"2010","journal-title":"Indian J Ophthalmol"},{"issue":"6","key":"pone.0257182.ref015","doi-asserted-by":"crossref","first-page":"e0180560","DOI":"10.1371\/journal.pone.0180560","article-title":"Seroprevalence of antibodies to dengue and chikungunya viruses in Thailand","volume":"12","author":"S Vongpunsawad","year":"2017","journal-title":"PLoS One"},{"issue":"23","key":"pone.0257182.ref016","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph17239134","article-title":"Dengue Seroprevalence and Seroconversion in Urban and Rural Populations in Northeastern Thailand and Southern Laos","volume":"17","author":"D Doum","year":"2020","journal-title":"Int J Environ Res Public Health"},{"issue":"6","key":"pone.0257182.ref017","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1086\/382280","article-title":"Relationship of preexisting dengue virus (DV) neutralizing antibody levels to viremia and severity of disease in a prospective cohort study of DV infection in Thailand","volume":"189","author":"TP Endy","year":"2004","journal-title":"J Infect Dis"},{"issue":"5","key":"pone.0257182.ref018","doi-asserted-by":"crossref","first-page":"825","DOI":"10.4269\/ajtmh.2009.08-0625","article-title":"Dengue plaque reduction neutralization test (PRNT) in primary and secondary dengue virus infections: How alterations in assay conditions impact performance","volume":"81","author":"SJ Thomas","year":"2009","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"pone.0257182.ref019","doi-asserted-by":"crossref","first-page":"e0213451","DOI":"10.1371\/journal.pone.0213451","article-title":"Comparative evaluation of three dengue duo rapid test kits to detect NS1, IgM, and IgG associated with acute dengue in children in Myanmar","volume":"14","author":"WS Jang","year":"2019","journal-title":"PLoS One"},{"key":"pone.0257182.ref020","volume-title":"Guidelines for plaque reduction neutralization testing of human antibodies to dengue viruses","author":"World Health Organization","year":"2007"},{"issue":"7","key":"pone.0257182.ref021","doi-asserted-by":"crossref","first-page":"e1732","DOI":"10.1371\/journal.pntd.0001732","article-title":"Dengue infection in children in Ratchaburi, Thailand: a cohort study. I. Epidemiology of symptomatic acute dengue infection in children, 2006\u20132009","volume":"6","author":"A Sabchareon","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"pone.0257182.ref022","doi-asserted-by":"crossref","first-page":"e3241","DOI":"10.1371\/journal.pntd.0003241","article-title":"Epidemiological trends of dengue disease in Thailand (2000\u20132011): a systematic literature review","volume":"8","author":"K Limkittikul","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0257182.ref023","first-page":"1","article-title":"Annual Changes of Predominant Dengue Virus Serotypes in Six Regional Hospitals in Thailand from 1999 to 2002","volume":"28","author":"S Anantapreecha","year":"2004","journal-title":"Dengue Bulletin"},{"issue":"5","key":"pone.0257182.ref024","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1097\/INF.0b013e3181646d45","article-title":"Age-specific prevalence of dengue antibodies in Bangkok infants and children","volume":"27","author":"K Pengsaa","year":"2008","journal-title":"Pediatr Infect Dis J"},{"issue":"2","key":"pone.0257182.ref025","doi-asserted-by":"crossref","first-page":"e0003396","DOI":"10.1371\/journal.pntd.0003396","article-title":"Neutralizing dengue antibody in pregnant Thai women and cord blood","volume":"9","author":"K Khamim","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"36","key":"pone.0257182.ref026","doi-asserted-by":"crossref","first-page":"5137","DOI":"10.1016\/j.vaccine.2019.07.016","article-title":"Pre-vaccination screening strategies for the use of the CYD-TDV dengue vaccine: A meeting report","volume":"37","author":"A Wilder-Smith","year":"2019","journal-title":"Vaccine"},{"issue":"3","key":"pone.0257182.ref027","doi-asserted-by":"crossref","first-page":"114918","DOI":"10.1016\/j.diagmicrobio.2019.114918","article-title":"Evaluation of dengue serological tests available in Puerto Rico for identification of prior dengue infection for prevaccination screening","volume":"96","author":"M Bonaparte","year":"2020","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"2","key":"pone.0257182.ref028","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/S1473-3099(18)30799-0","article-title":"Dengue pre-vaccination screening and positive predictive values","volume":"19","author":"I Rodr\u00edguez-Barraquer","year":"2019","journal-title":"Lancet Infect Dis"},{"issue":"5","key":"pone.0257182.ref029","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S1473-3099(19)30167-7","article-title":"Sensitivity and negative predictive value for a rapid dengue test","volume":"19","author":"S Flasche","year":"2019","journal-title":"Lancet Infect Dis"},{"issue":"6","key":"pone.0257182.ref030","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.cmi.2020.08.026","article-title":"Evaluation of a new point-of-care test to determine prior dengue infection for potential use in pre-vaccination screening","volume":"27","author":"JV Daag","year":"2020","journal-title":"Clin Microbiol Infect"},{"key":"pone.0257182.ref031","volume-title":"Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition","author":"World Health Organization","year":"2009"},{"issue":"2","key":"pone.0257182.ref032","first-page":"1","article-title":"New initiatives for the control of Japanese encephalitis by vaccination: minutes of a WHO\/CVI meeting, Bangkok, Thailand, 13\u201315 October 1998","volume":"18","author":"TF Tsai","year":"2000","journal-title":"Vaccine"},{"issue":"10","key":"pone.0257182.ref033","doi-asserted-by":"crossref","first-page":"e888","DOI":"10.1016\/j.ijid.2010.03.022","article-title":"Japanese encephalitis virus remains an important cause of encephalitis","volume":"14","author":"SJ Olsen","year":"2010","journal-title":"Thailand. Int J Infect Dis"},{"issue":"4","key":"pone.0257182.ref034","first-page":"725","article-title":"Antibody responses determined for Japanese dengue fever patients by neutralization and hemagglutination inhibition assays demonstrate cross-reactivity between dengue and Japanese encephalitis viruses","volume":"10","author":"K Yamada","year":"2003","journal-title":"Clin Diagn Lab Immunol"},{"issue":"10","key":"pone.0257182.ref035","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/j.jiac.2019.04.003","article-title":"Analysis of cross-reactivity between flaviviruses with sera of patients with Japanese encephalitis showed the importance of neutralization tests for the diagnosis of Japanese encephalitis","volume":"25","author":"T Maeki","year":"2019","journal-title":"J Infect Chemother"},{"issue":"1","key":"pone.0257182.ref036","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1186\/s12879-016-1873-8","article-title":"Japanese encephalitis vaccine-facilitated dengue virus infection-enhancement antibody in adults","volume":"16","author":"Y Saito","year":"2016","journal-title":"BMC Infect Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0257182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:13:37Z","timestamp":1673223217000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0257182"}},"subtitle":[],"editor":[{"given":"Ray","family":"Borrow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,9,10]]},"references-count":36,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,9,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0257182","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,10]]}}}