{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T09:32:09Z","timestamp":1753522329928,"version":"3.40.5"},"reference-count":24,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T00:00:00Z","timestamp":1631750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.<\/jats:p>","DOI":"10.1371\/journal.pone.0257416","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T17:34:01Z","timestamp":1631813641000},"page":"e0257416","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3053-8373","authenticated-orcid":true,"given":"Alireza","family":"Saraji","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Offermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janine","family":"Stegmann-Frehse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Hempel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duan","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemarie","family":"Krupar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Watermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny","family":"Jonigk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark Philipp","family":"K\u00fchnel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jutta","family":"Kirfel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sven","family":"Perner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Sailer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"issue":"1","key":"pone.0257416.ref001","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21551","article-title":"Cancer statistics, 2019","volume":"69","author":"RL Siegel","year":"2019","journal-title":"CA Cancer J Clin."},{"key":"pone.0257416.ref002","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.15382.1","article-title":"Recent trends in the management of advanced prostate cancer","volume":"7","author":"C Ritch","year":"2018","journal-title":"F1000Res"},{"issue":"10","key":"pone.0257416.ref003","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1002\/(SICI)1097-0142(19960515)77:10<2162::AID-CNCR30>3.0.CO;2-R","article-title":"The National Cancer Data Base report on longitudinal observations on prostate cancer","volume":"77","author":"CJ Mettlin","year":"1996","journal-title":"Cancer"},{"issue":"709","key":"pone.0257416.ref004","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1259\/0007-1285-60-709-79","article-title":"Staging of prostatic carcinoma with radionuclide bone scintigraphy and lymphography","volume":"60","author":"SJ Hayward","year":"1987","journal-title":"Br J Radiol"},{"issue":"2","key":"pone.0257416.ref005","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.eururo.2014.07.020","article-title":"Impact of the Site of Metastases on Survival in Patients with Metastatic Prostate Cancer","volume":"68","author":"G Gandaglia","year":"2015","journal-title":"Eur Urol"},{"issue":"14","key":"pone.0257416.ref006","first-page":"1362","article-title":"Evolution of treatment options for patients with CRPC and bone metastases: bone-targeted agents that go beyond palliation of symptoms to improve overall survival","volume":"25","author":"KO Rove","year":"2011","journal-title":"Oncology (Williston Park)"},{"issue":"11","key":"pone.0257416.ref007","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1586\/14737140.2014.972948","article-title":"Precision medicine for prostate cancer","volume":"14","author":"M Galazi","year":"2014","journal-title":"Expert Rev Anticancer Ther"},{"issue":"50","key":"pone.0257416.ref008","doi-asserted-by":"crossref","first-page":"88211","DOI":"10.18632\/oncotarget.21085","article-title":"Molecular determinants of prostate cancer metastasis","volume":"8","author":"K Rycaj","year":"2017","journal-title":"Oncotarget"},{"issue":"12","key":"pone.0257416.ref009","first-page":"3924","article-title":"Characterization of bone metastases from rapid autopsies of prostate cancer patients. 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