{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:13:19Z","timestamp":1772212399836,"version":"3.50.1"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["ALW OPEN 2015.091"],"award-info":[{"award-number":["ALW OPEN 2015.091"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["X-omics"],"award-info":[{"award-number":["X-omics"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["KWF 2015-8082"],"award-info":[{"award-number":["KWF 2015-8082"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["KWF 2015-8082"],"award-info":[{"award-number":["KWF 2015-8082"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Oncode Institute"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>BRCA1-associated protein 1 (BAP1) is a tumor suppressor and its loss can result in mesothelioma, uveal and cutaneous melanoma, clear cell renal cell carcinoma and bladder cancer. BAP1 is a deubiquitinating enzyme of the UCH class that has been implicated in various cellular processes like cell growth, cell cycle progression, ferroptosis, DNA damage response and ER metabolic stress response. ASXL proteins activate BAP1 by forming the polycomb repressive deubiquitinase (PR-DUB) complex which acts on H2AK119ub1. Besides the ASXL proteins, BAP1 is known to interact with an established set of additional proteins. Here, we identify novel BAP1 interacting proteins in the cytoplasm by expressing GFP-tagged BAP1 in an endogenous BAP1 deficient cell line using affinity purification followed by mass spectrometry (AP-MS) analysis. Among these novel interacting proteins are Histone acetyltransferase 1 (HAT1) and all subunits of the heptameric coat protein complex I (COPI) that is involved in vesicle formation and protein cargo binding and sorting. We validate that the HAT1 and COPI interactions occur at endogenous levels but find that this interaction with COPI is not mediated through the C-terminal KxKxx cargo sorting signals of the COPI complex.<\/jats:p>","DOI":"10.1371\/journal.pone.0257688","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T14:28:35Z","timestamp":1633012115000},"page":"e0257688","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Proteomic analysis identifies novel binding partners of BAP1"],"prefix":"10.1371","volume":"16","author":[{"given":"Roy","family":"Baas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8536-7359","authenticated-orcid":true,"given":"Fenna","family":"J. van der Wal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onno 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for H2A deubiquitination","volume":"7","author":"DD Sahtoe","year":"2016","journal-title":"Nat Commun"},{"issue":"48","key":"pone.0257688.ref003","doi-asserted-by":"crossref","first-page":"28643","DOI":"10.1074\/jbc.M115.661553","article-title":"The BAP1\/ASXL2 Histone H2A Deubiquitinase Complex Regulates Cell Proliferation and Is Disrupted in Cancer","volume":"290","author":"S Daou","year":"2015","journal-title":"J Biol Chem"},{"issue":"17","key":"pone.0257688.ref004","doi-asserted-by":"crossref","first-page":"6953","DOI":"10.1158\/0008-5472.CAN-08-0365","article-title":"BRCA1-associated protein-1 is a tumor suppressor that requires deubiquitinating activity and nuclear localization","volume":"68","author":"KH Ventii","year":"2008","journal-title":"Cancer Res"},{"issue":"3","key":"pone.0257688.ref005","doi-asserted-by":"crossref","first-page":"270","DOI":"10.21037\/tlcr.2017.05.03","article-title":"BAP1, a tumor suppressor gene driving malignant mesothelioma","volume":"6","author":"M Cheung","year":"2017","journal-title":"Transl Lung Cancer Res"},{"issue":"10","key":"pone.0257688.ref006","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1038\/ng.912","article-title":"Germline BAP1 mutations predispose to malignant mesothelioma","volume":"43","author":"JR Testa","year":"2011","journal-title":"Nat Genet"},{"issue":"6009","key":"pone.0257688.ref007","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1126\/science.1194472","article-title":"Frequent mutation of BAP1 in metastasizing uveal melanomas","volume":"330","author":"JW Harbour","year":"2010","journal-title":"Science"},{"issue":"10","key":"pone.0257688.ref008","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1038\/ng.910","article-title":"Germline mutations in BAP1 predispose to melanocytic tumors","volume":"43","author":"T Wiesner","year":"2011","journal-title":"Nat Genet"},{"issue":"7","key":"pone.0257688.ref009","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1002\/humu.22090","article-title":"Targeted exome sequencing in clear cell renal cell carcinoma tumors suggests aberrant chromatin regulation as a crucial step in ccRCC development","volume":"33","author":"G Duns","year":"2012","journal-title":"Hum Mutat"},{"issue":"7","key":"pone.0257688.ref010","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/ng.2323","article-title":"BAP1 loss defines a new class of renal cell carcinoma","volume":"44","author":"S Pena-Llopis","year":"2012","journal-title":"Nat Genet"},{"issue":"18","key":"pone.0257688.ref011","doi-asserted-by":"crossref","first-page":"4935","DOI":"10.1158\/1078-0432.CCR-14-0330","article-title":"Concurrent alterations in TERT, KDM6A, and the BRCA pathway in bladder cancer","volume":"20","author":"ML Nickerson","year":"2014","journal-title":"Clin Cancer 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