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The specific status of several species grouped in the<jats:italic>T<\/jats:italic>.<jats:italic>brasiliensis<\/jats:italic>complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between<jats:italic>T<\/jats:italic>.<jats:italic>lenti<\/jats:italic>and other species and subspecies of the<jats:italic>T<\/jats:italic>.<jats:italic>brasiliensis<\/jats:italic>complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between<jats:italic>T<\/jats:italic>.<jats:italic>b<\/jats:italic>.<jats:italic>brasiliensis<\/jats:italic>,<jats:italic>T<\/jats:italic>.<jats:italic>b<\/jats:italic>.<jats:italic>macromelasoma<\/jats:italic>,<jats:italic>T<\/jats:italic>.<jats:italic>juazeirensis<\/jats:italic>and<jats:italic>T<\/jats:italic>.<jats:italic>melanica<\/jats:italic>with<jats:italic>T<\/jats:italic>.<jats:italic>lenti<\/jats:italic>, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between<jats:italic>T<\/jats:italic>.<jats:italic>lenti<\/jats:italic>and the species and subspecies of the<jats:italic>T<\/jats:italic>.<jats:italic>brasiliensis<\/jats:italic>complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between<jats:italic>T<\/jats:italic>.<jats:italic>lenti<\/jats:italic>and species and subspecies of the<jats:italic>T<\/jats:italic>.<jats:italic>brasiliensis<\/jats:italic>complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.<\/jats:p>","DOI":"10.1371\/journal.pone.0257992","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T18:16:49Z","timestamp":1634321809000},"page":"e0257992","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex"],"prefix":"10.1371","volume":"16","author":[{"given":"Heloisa","family":"Pinotti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jader","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Ravazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Fernandez","family":"Madeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yago Visinho","family":"dos Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Beatriz Bortolozo","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Terc\u00edlia Vilela de","family":"Azeredo-Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Aristeu da","family":"Rosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-3764","authenticated-orcid":true,"given":"Kaio Cesar Chaboli","family":"Alevi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,10,15]]},"reference":[{"key":"pone.0257992.ref001","unstructured":"WHO (World Health Organizarion). 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