{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:06:31Z","timestamp":1790708791682,"version":"4.1.0"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T00:00:00Z","timestamp":1634083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009752","name":"karen c. drayer wildlife health center","doi-asserted-by":"publisher","award":["G16BS"],"award-info":[{"award-number":["G16BS"]}],"id":[{"id":"10.13039\/100009752","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011119","name":"College of Veterinary Medicine, University of Georgia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011119","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Chlamydial infections, caused by a group of obligate, intracellular, gram-negative bacteria, have health implications for animals and humans. Due to their highly infectious nature and zoonotic potential, staff at wildlife rehabilitation centers should be educated on the clinical manifestations, prevalence, and risk factors associated with <jats:italic>Chlamydia<\/jats:italic> spp. infections in raptors. The objectives of this study were to document the prevalence of chlamydial DNA shedding and anti-chlamydial antibodies in raptors admitted to five wildlife rehabilitation centers in California over a one-year period. Chlamydial prevalence was estimated in raptors for each center and potential risk factors associated with infection were evaluated, including location, species, season, and age class. Plasma samples and conjunctiva\/choana\/cloaca swabs were collected for serology and qPCR from a subset of 263 birds of prey, representing 18 species. Serologic assays identified both anti-<jats:italic>C<\/jats:italic>. <jats:italic>buteonis<\/jats:italic> IgM and anti-chlamydial IgY antibodies. Chlamydial DNA and anti-chlamydial antibodies were detected in 4.18% (11\/263) and 3.14% (6\/191) of patients, respectively. Chamydial DNA was identified in raptors from the families Accipitridae and Strigidae while anti-<jats:italic>C<\/jats:italic>.<jats:italic>buteonis<\/jats:italic> IgM was identified in birds identified in Accipitridae, Falconidae, Strigidae, and Cathartidae. Two of the chlamydial DNA positive birds (one Swainson\u2019s hawk (<jats:italic>Buteo swainsoni<\/jats:italic>) and one red-tailed hawk (<jats:italic>Buteo jamaicensis<\/jats:italic>)) were necropsied, and tissues were collected for culture. Sequencing of the cultured elementary bodies revealed a chlamydial DNA sequence with 99.97% average nucleotide identity to the recently described <jats:italic>Chlamydia buteonis<\/jats:italic>. Spatial clusters of seropositive raptors and raptors positive for chlamydial DNA were detected in northern California. Infections were most prevalent during the winter season. Furthermore, while the proportion of raptors testing positive for chlamydial DNA was similar across age classes, seroprevalence was highest in adults. This study questions the current knowledge on <jats:italic>C<\/jats:italic>. <jats:italic>buteonis<\/jats:italic> host range and highlights the importance of further studies to evaluate the diversity and epidemiology of <jats:italic>Chlamydia<\/jats:italic> spp. infecting raptor populations.<\/jats:p>","DOI":"10.1371\/journal.pone.0258500","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T18:51:22Z","timestamp":1634151082000},"page":"e0258500","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5721-5382","authenticated-orcid":true,"given":"Brittany A.","family":"Seibert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael 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titers","volume":"97","author":"GD Bossart","year":"2011","journal-title":"Dis Aquat Organ"},{"key":"pone.0258500.ref039","volume-title":"R: A language and environment for statistical computing","author":"RC Team","year":"2014"},{"issue":"8","key":"pone.0258500.ref040","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1002\/sim.4780140809","article-title":"Spatial disease clusters: detection and inference","volume":"14","author":"M Kulldorff","year":"1995","journal-title":"Statistics in medicine"},{"issue":"4","key":"pone.0258500.ref041","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1637\/10105-022812-ResNote.1","article-title":"Chlamydia psittaci in Swedish wetland birds: a risk to zoonotic infection","volume":"56","author":"M Blomqvist","year":"2012","journal-title":"Avian Dis"},{"issue":"1","key":"pone.0258500.ref042","doi-asserted-by":"crossref","first-page":"145","DOI":"10.7589\/0090-3558-37.1.145","article-title":"Seroepizootiology of selected infectious 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