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Spectroscopic analyses characterized 5 sterols as 29-Hydroperoxy-stigmasta-5,24(28)-dien-3\u03b2-ol, saringosterol (24-vinyl-cholest-5-ene-3\u03b2,24-diol), 24-methylenecholesterol, fucosterol (stigmasta-5,24-diene-3\u03b2-ol), and 24-Hydroperoxy-24-vinyl-cholesterol. The bioactivities of these sterols were tested using lipid peroxidation (LPO) and cyclooxygenase (COX-1 and -2) enzyme inhibitory assays. Fucosterol exhibited the highest COX-1 and -2 enzyme inhibitory activities at 59 and 47%, respectively. Saringosterol, 24-methylenecholesterol and fucosterol showed higher LPO inhibitory activity at &gt;50% than the other compounds. In addition, the results of molecular docking revealed that the 5 sterols were located in different pocket of COX-1 and -2 and fucosterol with tetracyclic skeletons and olefin methine achieved the highest binding energy (-7.85 and -9.02 kcal\/mol) through hydrophobic interactions and hydrogen bond. Our results confirm the presence of 5 sterols in\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>japonica<\/jats:italic>\n                    and its significant anti-inflammatory and antioxidant activity.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0258980","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T13:42:41Z","timestamp":1643290961000},"page":"e0258980","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["The edible seaweed Laminaria japonica contains cholesterol analogues that inhibit lipid peroxidation and cyclooxygenase enzymes"],"prefix":"10.1371","volume":"17","author":[{"given":"Xingyu","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amila 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