{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T03:57:10Z","timestamp":1771991830759,"version":"3.50.1"},"reference-count":12,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K01AI136725"],"award-info":[{"award-number":["K01AI136725"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K01AI136725"],"award-info":[{"award-number":["K01AI136725"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Recovery of <jats:italic>Neisseria gonorrhoeae<\/jats:italic> isolates exposed to a range of transport times and temperatures was quantitatively assessed for two transport devices, BioMed Diagnostics\u2019 InTray GC\u00ae and Copan Diagnostics\u2019 Liquid Amies Elution Swab (ESwab\u00ae) Collection and Transport System. Respective devices were inoculated with <jats:italic>N<\/jats:italic>. <jats:italic>gonorrhoeae<\/jats:italic>, exposed to simulated transport conditions and spread-plated from serial dilutions in duplicates onto chocolate agar in order to count CFU (colony-forming units) in the range of 25\u2013250. Baseline CFU\/mL averages of time-zero transport for each device was compared to either 24 hour (Eswab) or 72 hour (InTray GC) CFU\/mL average to assess recovery of six clinical isolates of <jats:italic>N<\/jats:italic>. <jats:italic>gonorrhoeae<\/jats:italic>, and differences showing no greater than a 3 log<jats:sub>10<\/jats:sub> (\u00b1 10%) decline between comparative time points qualified as acceptable. Our findings suggest that the InTray GC system has the potential to transport clinical isolates for \u226472 hours with acceptable <jats:italic>N<\/jats:italic>. <jats:italic>gonorrhoeae<\/jats:italic> recovery.<\/jats:p>","DOI":"10.1371\/journal.pone.0259668","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T18:37:52Z","timestamp":1642790272000},"page":"e0259668","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["72-Hour transport recovery of antimicrobial resistant Neisseria gonorrhoeae isolates using the InTray\u00ae GC method"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1403-3845","authenticated-orcid":true,"given":"Keely S.","family":"Paris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon","family":"Font","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay R.","family":"Mehta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irvin","family":"Huerta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire C.","family":"Bristow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,1,21]]},"reference":[{"issue":"9","key":"pone.0259668.ref001","doi-asserted-by":"crossref","first-page":"371","DOI":"10.15698\/mic2016.09.524","article-title":"Gonorrhea\u2014an evolving disease of the new millennium","volume":"3","author":"SA Hill","year":"2016","journal-title":"Microb Cell"},{"key":"pone.0259668.ref002","volume-title":"Sexually Transmitted Disease Surveillance 2018","author":"Centers for Disease Control and Prevention","year":"2019"},{"key":"pone.0259668.ref003","volume-title":"Report on global sexually transmitted disease surveillance","author":"World Health Organization","year":"2018"},{"issue":"50","key":"pone.0259668.ref004","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.15585\/mmwr.mm6950a6","article-title":"Update to CDC\u2019s Treatment Guidelines for Gonococcal Infection, 2020","volume":"69","author":"S St Cyr","year":"2020","journal-title":"MMWR Morb Mortal Wkly Rep."},{"issue":"1","key":"pone.0259668.ref005","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1155\/2005\/323082","article-title":"The laboratory diagnosis of Neisseria gonorrhoeae","volume":"16","author":"LK Ng","year":"2005","journal-title":"Can J Infect Dis Med Microbiol"},{"issue":"10","key":"pone.0259668.ref006","article-title":"Detection in the United Kingdom of the Neisseria gonorrhoeae FC428 clone, with ceftriaxone resistance and intermediate resistance to azithromycin, October to December 2018","volume":"24","author":"DW Eyre","year":"2019","journal-title":"Euro Surveill"},{"issue":"2","key":"pone.0259668.ref007","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.diagmicrobio.2016.06.019","article-title":"Recovery of Neisseria gonorrhoeae from 4 commercially available transport systems","volume":"86","author":"JR Papp","year":"2016","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"10","key":"pone.0259668.ref008","doi-asserted-by":"crossref","first-page":"3825","DOI":"10.1128\/JCM.38.10.3825-3826.2000","article-title":"InTray GC medium versus modified Thayer-Martin agar plates for diagnosis of gonorrhea from endocervical specimens","volume":"38","author":"A Beverly","year":"2000","journal-title":"J Clin Microbiol"},{"key":"pone.0259668.ref009","volume-title":"Antibiotic Resistance Isolate Bank","author":"CDC & FDA"},{"key":"pone.0259668.ref010","volume-title":"Quality Control of Microbiological Transport Systems; Approved Standard\u2014Second Edition, vol CLSI document M40-A2.","author":"CLSI","year":"2014"},{"key":"pone.0259668.ref011","volume-title":"Copan Liquid Amies Elution Swab (ESwab) Collection and Transport System [package insert].","year":"2017"},{"key":"pone.0259668.ref012","volume-title":"InTray GC [package insert]","author":"BioMed Diagnostics Inc","year":"2019"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0259668","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T18:38:18Z","timestamp":1642790298000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0259668"}},"subtitle":[],"editor":[{"given":"Supram","family":"Hosuru Subramanya","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,1,21]]},"references-count":12,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0259668","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,21]]}}}