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The aim of this study was to perform molecular characterization of<jats:italic>L<\/jats:italic>.<jats:italic>monocytogenes<\/jats:italic>and<jats:italic>Salmonella<\/jats:italic>spp. isolated from biofilms of cattle and poultry slaughterhouses located in the Federal District and State of Goi\u00e1s, Brazil. Fourteen<jats:italic>L<\/jats:italic>.<jats:italic>monocytogenes<\/jats:italic>isolates and one<jats:italic>Salmonella<\/jats:italic>sp. were detected in poultry slaughterhouses. No isolates were detected in cattle slaughterhouses. All<jats:italic>L<\/jats:italic>.<jats:italic>monocytogenes<\/jats:italic>isolates belonged to lineage II, and 11 different pulsotypes were detected. Pulsed-field gel electrophoresis analysis revealed the dissemination of two strains within one plant, in addition to the regional dissemination of one of them. The<jats:italic>Salmonella<\/jats:italic>isolate was identified via whole genome sequencing as<jats:italic>Salmonella enterica<\/jats:italic>serovar Minnesota ST548. In the sequence analysis, no premature stop codons were detected in the<jats:italic>inlA<\/jats:italic>gene of<jats:italic>Listeria<\/jats:italic>. All isolates demonstrated the ability to adhere to Caco-2 cells, while 50% were capable of invading them. Antimicrobial resistance was detected in 57.1% of the<jats:italic>L<\/jats:italic>.<jats:italic>monocytogenes<\/jats:italic>isolates, and resistance to sulfonamide was the most common feature. The<jats:italic>tetC<\/jats:italic>,<jats:italic>ermB<\/jats:italic>, and<jats:italic>tetM<\/jats:italic>genes were detected, and four isolates were classified as multidrug-resistant.<jats:italic>Salmonella<\/jats:italic>sp. was resistant to nine antimicrobials and was classified as multidrug-resistant. Resistance genes<jats:italic>qnrB19<\/jats:italic>,<jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CMY-2<\/jats:italic><\/jats:sub>,<jats:italic>aac(6\u2019)-Iaa<\/jats:italic>,<jats:italic>sul2<\/jats:italic>, and<jats:italic>tetA<\/jats:italic>, and a mutation in the<jats:italic>parC<\/jats:italic>gene were detected. The majority (78.5%) of the<jats:italic>L<\/jats:italic>.<jats:italic>monocytogenes<\/jats:italic>isolates were capable of forming biofilms after incubation at 37\u00b0C for 24 h, and 64.3% were capable of forming biofilms after incubation at 12\u00b0C for 168 h. There was no statistical difference in the biofilm-forming capacity under the different evaluated conditions.<jats:italic>Salmonella<\/jats:italic>sp. was capable of forming biofilms at both tested temperatures. Biofilm characterization was confirmed by collecting the samples consistently, at the same sampling points, and by assessing biofilm formation<jats:italic>in vitro<\/jats:italic>. These results highlight the potential risk of cross-contamination in poultry slaughterhouses and the importance of surveillance and pathogen control maintenance programs within the meat production industry.<\/jats:p>","DOI":"10.1371\/journal.pone.0259687","type":"journal-article","created":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T18:31:37Z","timestamp":1636741897000},"page":"e0259687","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goi\u00e1s, Brazil"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4406-026X","authenticated-orcid":true,"given":"Emilia Fernanda","family":"Agostinho Davanzo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca Lavarini","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virgilio Hip\u00f3lito de Lemos","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4958-2569","authenticated-orcid":true,"given":"Joana Marchesini","family":"Palma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Rocha","family":"Pribul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno St\u00e9fano Lima","family":"Dallago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Fuga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7156-2460","authenticated-orcid":true,"given":"Margareti","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simoneide Souza","family":"Titze de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayanna Maria Boaventura","family":"da Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e1lia dos Prazeres","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Perecmanis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4969-9831","authenticated-orcid":true,"given":"Angela Patr\u00edcia","family":"Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,11,12]]},"reference":[{"issue":"5","key":"pone.0259687.ref001","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1111\/1541-4337.12283","article-title":"Residential Bacteria on Surfaces in the Food Industry and Their Implications for Food Safety and Quality","volume":"16","author":"T M\u00f8retr\u00f8","year":"2017","journal-title":"Compr Rev Food Sci Food Saf"},{"key":"pone.0259687.ref002","doi-asserted-by":"crossref","first-page":"150","DOI":"10.4315\/0362-028X.JFP-13-150","article-title":"Listeria monocytogenes persistence in food-associated environments: Epidemiology, strain characteristics, and implications for public health","volume":"77","author":"V Ferreira","year":"2014","journal-title":"Journal of Food Protection. 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