{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:29:44Z","timestamp":1790922584636,"version":"4.1.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T00:00:00Z","timestamp":1635984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["F.No. 41-1145\/2012(SR)"],"award-info":[{"award-number":["F.No. 41-1145\/2012(SR)"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["F.No. 2015-17\/ RGNF-2015-17-TEL-1236"],"award-info":[{"award-number":["F.No. 2015-17\/ RGNF-2015-17-TEL-1236"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004541","name":"Ministry of Human Resource Development","doi-asserted-by":"publisher","award":["RUSA 2.0"],"award-info":[{"award-number":["RUSA 2.0"]}],"id":[{"id":"10.13039\/501100004541","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The present study describes the probiotic potential and functional properties of the lactic acid bacterium\n                    <jats:italic>Pediococcus pentosaceus<\/jats:italic>\n                    OBK05 isolated from buttermilk. The isolate OBK05 was assessed for its probiotic properties. The isolate showed notable tolerance to pH 2.0 and 3.0 (8.44, 8.35 log CFU\/mL), oxbile of 0.5% at 2 and 4 h of incubation (6.97, 6.35 log CFU\/mL) and higher aggregation (auto-aggregation, adhesion to hydrocarbons) than the referral strain,\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    MTCC 10307. The adhesion efficiency to HT-29 cells was found to be maximum, corresponding to 93.5% and 97% at 1 and 2 h incubation, respectively. In addition, the isolate OBK05 showed antagonistic solid activity against bacterial pathogens like\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    MTCC 424 and\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    MTCC 1133. The phenotypic antibiotic resistance of the isolate was examined before and after curing plasmids. Among the known five structural genes responsible for different antibiotic resistance, four genes indicating antibiotic resistance to kanamycin-\n                    <jats:italic>Aph<\/jats:italic>\n                    (3\u00b4\u00b4)-III, streptomycin-\n                    <jats:italic>strA<\/jats:italic>\n                    , vancomycin-\n                    <jats:italic>vanA<\/jats:italic>\n                    and ciprofloxacin-\n                    <jats:italic>gyrA<\/jats:italic>\n                    were detected by PCR amplification of genomic DNA. Further, the horizontal gene transfer from OBK05 isolate to pathogens was not found for these antibiotic resistance markers when filter and food mating were carried out as no transconjugants developed on media plates containing respective antibiotics. This indicates that the intrinsic resistance is harbored on chromosomal genes, and hence it is nontransferable to other microbes. In addition, strain OBK05 exhibited good DPPH scavenging activity of 56 to 77% and liberated free amino acid from conjugated bile acid. The strain OBK05 demonstrated a strong ability to reduce cholesterol at 12 h (17%), 24 h (27%) and 48 h (67%) of incubation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0259702","type":"journal-article","created":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T13:33:52Z","timestamp":1636032832000},"page":"e0259702","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Unraveling the probiotic efficiency of bacterium Pediococcus pentosaceus OBK05 isolated from buttermilk: An in vitro study for cholesterol assimilation potential and antibiotic resistance status"],"prefix":"10.1371","volume":"16","author":[{"given":"Kiran Kumar","family":"Bhukya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1343-3863","authenticated-orcid":true,"given":"Bhima","family":"Bhukya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,11,4]]},"reference":[{"key":"pone.0259702.ref001","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s13205-019-1841-2","article-title":"Bioactive molecules of probiotic bacteria and their mechanism of action: a review.","volume":"9","author":"M Indira","year":"2019","journal-title":"3 Biotech."},{"key":"pone.0259702.ref002","doi-asserted-by":"crossref","first-page":"502","DOI":"10.3389\/fmicb.2019.00502","article-title":"Mechanistic Insights into Probiotic Properties of Lactic Acid Bacteria Associated With Ethnic Fermented Dairy Products.","volume":"10","author":"G Tamoghna","year":"2019","journal-title":"Front Microbiol."},{"key":"pone.0259702.ref003","article-title":"Growth and metabolic characterization of four lactic acid bacteria species isolated from rice beer prepared in AssamIndia","volume":"1","author":"AJ Das","year":"2019","journal-title":"Access Microbiology"},{"key":"pone.0259702.ref004","doi-asserted-by":"crossref","first-page":"202","DOI":"10.3390\/dairy1030015","article-title":"Lactic Acid Bacteria: Food Safety and Human Health Applications","volume":"1","author":"DA Raphael","year":"2020","journal-title":"Dairy."},{"key":"pone.0259702.ref005","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.lwt.2017.12.063","article-title":"Probiotic properties and stress response of thermotolerant lactic acid bacteria isolated from cooked meat products.","volume":"91","author":"AM Hern\u00e1ndez-Alc\u00e1ntara","year":"2018","journal-title":"LWT."},{"key":"pone.0259702.ref006","doi-asserted-by":"crossref","first-page":"768","DOI":"10.3389\/fmicb.2015.00768","article-title":"Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7 and in vivo assessment of its cholesterol-lowering activity","volume":"6","author":"K Damodharan","year":"2015","journal-title":"Front Microbiol"},{"key":"pone.0259702.ref007","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/jam.13339","article-title":"Selection of a candidate probiotic strain of Pediococcus pentosaceus from the faecal microbiota of horses by in vitro testing and health claims in a mouse model of Salmonella infection","volume":"122","author":"BC Silva","year":"2017","journal-title":"J Appl Microbiol"},{"key":"pone.0259702.ref008","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0147960","article-title":"Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells","volume":"11","author":"A Tiptiri-Kourpeti","year":"2016","journal-title":"PloS one"},{"key":"pone.0259702.ref009","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1016\/j.biotechadv.2018.09.003","article-title":"How to select a probiotic? 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