{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:24:37Z","timestamp":1760300677121,"version":"3.37.3"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["221047\/F40"],"award-info":[{"award-number":["221047\/F40"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014781","name":"Gjensidigestiftelsen","doi-asserted-by":"crossref","award":["1042294"],"award-info":[{"award-number":["1042294"]}],"id":[{"id":"10.13039\/501100014781","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Aerobic fitness (AF) and lipoprotein subclasses associate to each other and to cardiovascular health. Adiposity and physical activity (PA) influence the association pattern of AF to lipoproteins almost inversely making it difficult to assess their independent and joint influence on the association pattern. This study, including 841 children (50% boys) 10.2 \u00b1 0.3 years old with BMI 18.0 \u00b1 3.0 kg\/m2 from rural Western Norway, aimed at examining the association pattern of AF to the lipoprotein subclasses and to estimate the independent and joint influence of PA and adiposity on this pattern. We used multivariate analysis to determine the association pattern of a profile of 26 lipoprotein features to AF with and without adjustment for three measures of adiposity and a high-resolution PA descriptor of 23 intensity intervals derived from accelerometry. For data not adjusted for adiposity or PA, we observed a cardioprotective lipoprotein pattern associating to AF. This pattern withstood adjustment for PA, but the strength of association to AF was reduced by 58%, while adjustment for adiposity weakened the association of AF to the lipoproteins by 85% and with strongest changes in the associations to a cardioprotective high-density lipoprotein subclass pattern. When adjusted for both adiposity and PA, the cardioprotective lipoprotein pattern still associated to AF, but the strength of association was reduced by 90%. Our results imply that the (negative) influence of adiposity on the cardioprotective association pattern of lipoproteins to AF is considerably stronger than the (positive) contribution of PA to this pattern. However, our analysis shows that PA contributes also indirectly through a strong inverse association to adiposity. The trial was registered 7 May, 2014 in clinicaltrials.gov with trial reg. no.: NCT02132494 and the URL is <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/ct2\/results?term=NCT02132494&amp;cntry=NO\" xlink:type=\"simple\">https:\/\/clinicaltrials.gov\/ct2\/results?term=NCT02132494&amp;cntry=NO<\/jats:ext-link>.<\/jats:p>","DOI":"10.1371\/journal.pone.0259901","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T18:56:36Z","timestamp":1637261796000},"page":"e0259901","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Influence of adiposity and physical activity on the cardiometabolic association pattern of lipoprotein subclasses to aerobic fitness in prepubertal children"],"prefix":"10.1371","volume":"16","author":[{"given":"Tarja","family":"Rajalahti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eivind","family":"Aadland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geir K\u00e5re","family":"Resaland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigmund Alfred","family":"Anderssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9432-8776","authenticated-orcid":true,"given":"Olav Martin","family":"Kvalheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"key":"pone.0259901.ref001","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1001\/jama.2009.681","article-title":"Cardiorespiratory fitness as a quantitative predictor of all-cause mortality and cardiovascular events in healthy men and women: a meta-analysis","volume":"301","author":"S Kodama","year":"2009","journal-title":"JAMA"},{"key":"pone.0259901.ref002","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cca.2012.09.010","article-title":"Small dense LDL: an emerging risk factor for cardiovascular disease","volume":"414","author":"S Hirayama","year":"2012","journal-title":"Clin Chim Acta"},{"key":"pone.0259901.ref003","doi-asserted-by":"crossref","first-page":"453","DOI":"10.2337\/diabetes.52.2.453","article-title":"Effects of Insulin Resistance and Type 2 Diabetes on Lipoprotein Subclass Particle Size and Concentration Determined by Nuclear Magnetic Resonance","volume":"52","author":"WT Garvey","year":"2003","journal-title":"Diabetes"},{"key":"pone.0259901.ref004","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.metabol.2004.09.002","article-title":"Insulin resistance and adiposity influence lipoprotein size and subclass concentrations. 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J. Obesity"},{"key":"pone.0259901.ref009","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1002\/ajhb.20079","article-title":"Aerobics Center Longitudinal S. Stability of variables associated with the metabolic syndrome from adolescence to adulthood: The Aerobics Center Longitudinal Study","volume":"16","author":"JC Eisenmann","year":"2004","journal-title":"Am J Human Biol"},{"key":"pone.0259901.ref010","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1001\/jama.290.17.2277","article-title":"Cardiovascular risk factors in childhood and carotid artery intima-media thickness in adulthood: the Cardiovascular Risk in Young Finns Study","volume":"290","author":"OT Raitakari","year":"2003","journal-title":"JAMA"},{"key":"pone.0259901.ref011","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/sms.12897","article-title":"Strong association between cardiorespiratory fitness and lipoprotein subclass pattern in prepubertal healthy children.","volume":"28","author":"GK Resaland","year":"2018","journal-title":"Scand J Med Sci Sports"},{"key":"pone.0259901.ref012","first-page":"2095","volume":"13","author":"T Rajalahti","year":"2021","journal-title":"Cardiometabolic associations between physical activity, adiposity, and lipoprotein subclasses in prepubertal Norwegian children, Nutrients"},{"key":"pone.0259901.ref013","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1007\/s00125-007-0762-5","article-title":"Independent associations of physical activity and cardiorespiratory fitness with metabolic risk factors in children: the European youth heart study","volume":"50","author":"U Ekelund","year":"2007","journal-title":"Diabetologia"},{"key":"pone.0259901.ref014","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1056\/NEJMoa020194","article-title":"Effects of the amount and intensity of exercise on plasma lipoproteins","volume":"347","author":"WE Kraus","year":"2002","journal-title":"New England J Medicine"},{"key":"pone.0259901.ref015","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.metabol.2006.10.019","article-title":"Endurance exercise training raises 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Children","volume":"9","author":"E Aadland","year":"2014","journal-title":"Plos One."},{"key":"pone.0259901.ref021","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/s11306-015-0886-4","article-title":"Predictive associations between serum fatty acid and lipoproteins in healthy non-obese Norwegians\u2013implications for cardiovascular health.","volume":"12","author":"C Lin","year":"2016","journal-title":"Metabolomics"},{"key":"pone.0259901.ref022","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1161\/01.ATV.0000155017.60171.88","article-title":"Identification of Unique Lipoprotein Subclasses for Visceral Obesity by Component Analysis of Cholesterol Profile in High-Performance Liquid Chromatography","volume":"25","author":"M Okazaki","year":"2005","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"pone.0259901.ref023","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.ijpharm.2011.02.019","article-title":"Multivariate data analysis in pharmaceutics: A tutorial review","volume":"417","author":"T Rajalahti","year":"2011","journal-title":"Intern J Pharmaceutics"},{"key":"pone.0259901.ref024","doi-asserted-by":"crossref","first-page":"e2993","DOI":"10.1002\/cem.2993","article-title":"Determination of optimum number of components in partial least squares regression from distributions of the root-mean-squared error obtained by Monte Carlo resampling","volume":"32","author":"OM Kvalheim","year":"2018","journal-title":"J Chemometrics"},{"key":"pone.0259901.ref025","doi-asserted-by":"crossref","first-page":"9887","DOI":"10.1021\/ac5025039","article-title":"Precision High-Throughput Proton NMR Spectroscopy of Human Urine, Serum, and Plasma for Large-Scale Metabolic Phenotyping","volume":"86","author":"AC Dona","year":"2015","journal-title":"Anal Chem"},{"key":"pone.0259901.ref026","doi-asserted-by":"crossref","first-page":"S86","DOI":"10.1249\/MSS.0b013e3182399f5e","article-title":"Actigraph and Actical physical activity 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Sports Sciences"},{"key":"pone.0259901.ref028","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12889-018-5212-4","article-title":"A comparison of 10 accelerometer non-wear time criteria and logbooks in children","volume":"18","author":"E Aadland","year":"2018","journal-title":"BMC Public Health"},{"key":"pone.0259901.ref029","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12966-019-0836-z","article-title":"Multicollinear physical activity accelerometry data and associations to cardiometabolic health: Challenges, pitfalls, and potential solutions","volume":"16","author":"E Aadland","year":"2019","journal-title":"Int J Behav Nutr Phys Act"},{"key":"pone.0259901.ref030","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.chemolab.2008.08.004","article-title":"Biomarker discovery in mass spectral profiles by means of selectivity ratio plot.","volume":"95","author":"T Rajalahti","year":"2009","journal-title":"Chemometr & Intell Lab 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Health"},{"key":"pone.0259901.ref034","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/S0022-3476(05)81997-3","article-title":"Relation of percentage of body fat and maximal aerobic capacity to risk factors for atherosclerosis and diabetes in black and white seven- to eleven-year-old children","volume":"125","author":"B Gutin","year":"1994","journal-title":"J Pediatrics"},{"key":"pone.0259901.ref035","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1097\/HJR.0b013e32808c67e3","article-title":"Cardiorespiratory fitness relates more strongly than physical activity to cardiovascular disease risk factors in healthy children and adolescents: the European Youth Heart Study","volume":"14","author":"A Hurtig-Wennlof","year":"2007","journal-title":"Eur J Cardiovasc Prev Rehabil"},{"key":"pone.0259901.ref036","doi-asserted-by":"crossref","first-page":"e1002649","DOI":"10.1371\/journal.pmed.1002649","article-title":"Associations of device-measured physical activity across 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