{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:31:06Z","timestamp":1790469066734,"version":"4.1.0"},"reference-count":90,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002873","name":"chulalongkorn university","doi-asserted-by":"publisher","award":["GB-CU-61-12-30-02"],"award-info":[{"award-number":["GB-CU-61-12-30-02"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002873","name":"chulalongkorn university","doi-asserted-by":"publisher","award":["The 100th Anniversary Chulalongkorn University for Doctoral Scholarship"],"award-info":[{"award-number":["The 100th Anniversary Chulalongkorn University for Doctoral Scholarship"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002873","name":"chulalongkorn university","doi-asserted-by":"publisher","award":["The 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund"],"award-info":[{"award-number":["The 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004776","name":"faculty of medicine, chulalongkorn university","doi-asserted-by":"publisher","award":["Ratchadaphiseksomphot Endowment Fund (Matching Fund)"],"award-info":[{"award-number":["Ratchadaphiseksomphot Endowment Fund (Matching Fund)"]}],"id":[{"id":"10.13039\/501100004776","id-type":"DOI","asserted-by":"publisher"}]},{"name":"anr leptomove","award":["18-CE15-0027-01"],"award-info":[{"award-number":["18-CE15-0027-01"]}]},{"name":"France G\u00e9nomique and IBISA","award":["ANR-10-INBS-09-09"],"award-info":[{"award-number":["ANR-10-INBS-09-09"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Carbon Storage Regulator A (CsrA) is a well-characterized post-transcriptional global regulator that plays a critical role in response to environmental changes in many bacteria. CsrA has been reported to regulate several metabolic pathways, motility, biofilm formation, and virulence-associated genes. The role of <jats:italic>csrA<\/jats:italic> in <jats:italic>Leptospira<\/jats:italic> spp., which are able to survive in different environmental niches and infect a wide variety of reservoir hosts, has not been characterized. To investigate the role of <jats:italic>csrA<\/jats:italic> as a gene regulator in <jats:italic>Leptospira<\/jats:italic>, we generated a <jats:italic>L<\/jats:italic>. <jats:italic>biflexa csrA<\/jats:italic> deletion mutant (\u0394<jats:italic>csrA<\/jats:italic>) and <jats:italic>csrA<\/jats:italic> overexpressing <jats:italic>Leptospira<\/jats:italic> strains. The \u0394<jats:italic>csrA L<\/jats:italic>. <jats:italic>biflexa<\/jats:italic> displayed poor growth under starvation conditions. RNA sequencing revealed that in rich medium only a few genes, including the gene encoding the flagellar filament protein FlaB3, were differentially expressed in the \u0394<jats:italic>csrA<\/jats:italic> mutant. In contrast, 575 transcripts were differentially expressed when <jats:italic>csrA<\/jats:italic> was overexpressed in <jats:italic>L<\/jats:italic>. <jats:italic>biflexa<\/jats:italic>. Electrophoretic mobility shift assay (EMSA) confirmed the RNA-seq data in the \u0394<jats:italic>csrA<\/jats:italic> mutant, showing direct binding of recombinant CsrA to <jats:italic>flaB3<\/jats:italic> mRNA. In the pathogen <jats:italic>L<\/jats:italic>. <jats:italic>interrogans<\/jats:italic>, we were not able to generate a <jats:italic>csrA<\/jats:italic> mutant. We therefore decided to overexpress <jats:italic>csrA<\/jats:italic> in <jats:italic>L<\/jats:italic>. <jats:italic>interrogans<\/jats:italic>. In contrast to the overexpressing strain of <jats:italic>L<\/jats:italic>. <jats:italic>biflexa<\/jats:italic>, the overexpressing <jats:italic>L<\/jats:italic>. <jats:italic>interrogans<\/jats:italic> strain had poor motility on soft agar. The overexpressing strain of <jats:italic>L<\/jats:italic>. <jats:italic>interrogans<\/jats:italic> also showed significant upregulation of the flagellin <jats:italic>flaB1<\/jats:italic>, <jats:italic>flaB2<\/jats:italic>, and <jats:italic>flaB4<\/jats:italic>. The interaction of <jats:italic>L<\/jats:italic>. <jats:italic>interrogans<\/jats:italic> rCsrA and <jats:italic>flaB4<\/jats:italic> was confirmed by EMSA. Our results demonstrated that CsrA may function as a global regulator in <jats:italic>Leptospira<\/jats:italic> spp. under certain conditions that cause <jats:italic>csrA<\/jats:italic> overexpression. Interestingly, the mechanisms of action and gene targets of CsrA may be different between non-pathogenic and pathogenic <jats:italic>Leptospira<\/jats:italic> strains.<\/jats:p>","DOI":"10.1371\/journal.pone.0260981","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T18:26:52Z","timestamp":1639420012000},"page":"e0260981","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Investigating the role of the carbon storage regulator A (CsrA) in Leptospira spp."],"prefix":"10.1371","volume":"16","author":[{"given":"Theerapat","family":"Phoka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Fule","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Pipoli","family":"Da 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