{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T17:35:10Z","timestamp":1782495310918,"version":"3.54.5"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T00:00:00Z","timestamp":1639094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K18649"],"award-info":[{"award-number":["20K18649"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>This study aimed to investigate the effect of ozone ultrafine bubble water (OUFBW) on the formation and growth of <jats:italic>Candida albicans<\/jats:italic> (<jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic>) biofilms and surface properties of denture base resins. OUFBWs were prepared under concentrations of 6 (OUFBW6), 9 (OUFBW9), and 11 ppm (OUFBW11). Phosphate buffered saline and ozone-free electrolyte aqueous solutions (OFEAS) were used as controls. Acrylic resin discs were made according to manufacturer instructions, and <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> was initially cultured on the discs for 1.5 h. A colony forming unit (CFU) assay was performed by soaking the discs in OUFBW for 5 min after forming a 24-h <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> biofilm. The discs after initial attachment for 1.5 h were immersed in OUFBW and then cultured for 0, 3, and 5 h. CFUs were subsequently evaluated at each time point. Moreover, a viability assay, scanning electron microscopy (SEM), Alamar Blue assay, and quantitative real-time polymerase chain reaction (qRT-PCR) test were performed. To investigate the long-term effects of OUFBW on acrylic resin surface properties, Vickers hardness (VH) and surface roughness (Ra) were measured. We found that OUFBW9 and OUFBW11 significantly degraded the formed 24-h biofilm. The time point CFU assay showed that <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> biofilm formation was significantly inhibited due to OUFBW11 exposure. Interestingly, fluorescence microscopy revealed that almost living cells were observed in all groups. In SEM images, the OUFBW group had lesser number of fungi and the amount of non-three-dimensional biofilm than the control group. In the Alamar Blue assay, OUFBW11 was found to suppress <jats:italic>Candida<\/jats:italic> metabolic function. The qRT-PCR test showed that OUFBW down-regulated <jats:italic>ALS1<\/jats:italic> and <jats:italic>ALS3<\/jats:italic> expression regarding cell-cell, cell-material adhesion, and biofilm formation. Additionally, VH and Ra were not significantly different between the two groups. Overall, our data suggest that OUFBW suppressed <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> growth and biofilm formation on polymethyl methacrylate without impairing surface properties.<\/jats:p>","DOI":"10.1371\/journal.pone.0261180","type":"journal-article","created":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T18:26:56Z","timestamp":1639160816000},"page":"e0261180","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Ozone ultrafine bubble water inhibits the early formation of Candida albicans biofilms"],"prefix":"10.1371","volume":"16","author":[{"given":"Yuka","family":"Shichiri-Negoro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Tsutsumi-Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3337-1073","authenticated-orcid":true,"given":"Yuki","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhito","family":"Satomura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Arakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0517-6756","authenticated-orcid":true,"given":"Noriyuki","family":"Wakabayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,12,10]]},"reference":[{"key":"pone.0261180.ref001","doi-asserted-by":"crossref","first-page":"81","DOI":"10.4103\/0973-029X.141325","article-title":"Oral candidiasis: An overview","volume":"18","author":"A Singh","year":"2014","journal-title":"J Oral Maxillofac Pathol"},{"key":"pone.0261180.ref002","first-page":"54","article-title":"Candida albicans importance to denture wearers. 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Takahashi","year":"2021","journal-title":"Langmuir"},{"issue":"7","key":"pone.0261180.ref020","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s10103-019-02751-2","article-title":"Effectiveness of 405-nm blue LED light for degradation of Candida biofilms formed on PMMA denture base resin","volume":"34","author":"C Tsutsumi-Arai","year":"2019","journal-title":"Lasers Med Sci"},{"issue":"5","key":"pone.0261180.ref021","doi-asserted-by":"crossref","first-page":"e0217496","DOI":"10.1371\/journal.pone.0217496","article-title":"Grapefruit seed extract effectively inhibits the Candida albicans biofilms development on polymethyl methacrylate denture-base resin","volume":"14","author":"C Tsutsumi-Arai","year":"2019","journal-title":"PLoS One"},{"key":"pone.0261180.ref022","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jdent.2015.11.010","article-title":"Reduction of Candida biofilm adhesion by incorporation of prereacted glass ionomer filler in denture base resin","volume":"44","author":"C Tsutsumi","year":"2016","journal-title":"J Dent."},{"key":"pone.0261180.ref023","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.archoralbio.2017.12.024","article-title":"Superhydrophilic co-polymer coatings on denture surfaces reduce Candida albicans adhesion\u2014an in vitro study","volume":"87","author":"M Hirasawa","year":"2018","journal-title":"Arch Oral Biol"},{"key":"pone.0261180.ref024","first-page":"2018","article-title":"The effect of irradiation with a 405nm blue-violet laser on the bacterial adhesion on the osteosynthetic biomaterials","author":"C Terada","year":"2018","journal-title":"Hindawi Int J Photoenergy"},{"key":"pone.0261180.ref025","article-title":"Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin","author":"C Tsutsumi-Arai","year":"2021","journal-title":"Lasers Med Sci"},{"key":"pone.0261180.ref026","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0966-842X(01)01984-9","article-title":"The ALS gene family of Candida albicans","volume":"9","author":"LL Hoyer","year":"2001","journal-title":"Trends Microbiol"},{"issue":"11","key":"pone.0261180.ref027","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1111\/j.1462-5822.2005.00616.x","article-title":"Contributions of hyphae and hypha-co-regulated genes to Candida albicans virulence","volume":"7","author":"CA Kumamoto","year":"2005","journal-title":"Cell Microbiol"},{"key":"pone.0261180.ref028","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.sjdr.2016.06.005","article-title":"Dental applications of ozone therapy: a review of literature","volume":"8","author":"S Tiwari","year":"2017","journal-title":"Saudi J Dent Res"},{"key":"pone.0261180.ref029","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4103\/0976-9668.82319","article-title":"Ozone therapy: a clinical review","volume":"2","author":"AM Elvis","year":"2011","journal-title":"J Nat Sci Biol Med"},{"key":"pone.0261180.ref030","first-page":"1068","article-title":"Comparison of methods used for assessing the viability and vitality of yeast cells","volume":"14","author":"M Kwolek-Mirek","year":"2014","journal-title":"FEMS Yeast Res"},{"key":"pone.0261180.ref031","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1111\/j.1399-302X.2004.00146.x","article-title":"Efficacy of ozone on survival and permeability of oral microorganisms","volume":"19","author":"M Nagayoshi","year":"2004","journal-title":"Oral Microbiol Immunol"},{"key":"pone.0261180.ref032","doi-asserted-by":"crossref","first-page":"387","DOI":"10.29271\/jcpsp.2019.04.387","article-title":"Oxidizing effect of ozonated-water on microbial balance in the oral ecosystem","volume":"29","author":"FA Razak","year":"2019","journal-title":"J Coll Physicians Surg Pak"},{"issue":"3","key":"pone.0261180.ref033","doi-asserted-by":"crossref","first-page":"94","DOI":"10.15698\/mic2014.01.131","article-title":"Salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA","volume":"1","author":"Q Gao","year":"2014","journal-title":"Microb Cell"},{"key":"pone.0261180.ref034","first-page":"12004","volume":"509","author":"EF Karamah","year":"2019","journal-title":"The application of ozonated water to maintain the quality of tuna meat: the effect of contact time, contact temperature and ozone dosage"},{"key":"pone.0261180.ref035","doi-asserted-by":"crossref","first-page":"103813","DOI":"10.1016\/j.resmic.2021.103813","article-title":"Involvement of amyloid proteins in the formation of biofilms in the pathogenic yeast Candida albicans","volume":"172","author":"T Mourer","year":"2021","journal-title":"Res Microbiol"},{"key":"pone.0261180.ref036","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1128\/EC.00309-07","article-title":"Candida albicans Als adhesins have conserved amyloid-forming sequences","volume":"7","author":"HN Otoo","year":"2008","journal-title":"Eukaryot Cell"},{"key":"pone.0261180.ref037","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1128\/IAI.66.4.1783-1786.1998","article-title":"Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells","volume":"66","author":"Y Fu","year":"1998","journal-title":"Infect Immun"},{"key":"pone.0261180.ref038","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1080\/13693780701299333","article-title":"Candida albicans Als proteins mediate aggregation with bacteria and yeasts","volume":"45","author":"SA Klotz","year":"2007","journal-title":"Med Mycol"},{"key":"pone.0261180.ref039","doi-asserted-by":"crossref","first-page":"e1005522","DOI":"10.1371\/journal.ppat.1005522","article-title":"Candida glabrata binding to Candida albicans hyphae enables its development in oropharyngeal candidiasis","volume":"12","author":"S Tati","year":"2016","journal-title":"PLOS Pathog"},{"key":"pone.0261180.ref040","doi-asserted-by":"crossref","first-page":"e1000217","DOI":"10.1371\/journal.ppat.1000217","article-title":"The hyphal-associated adhesin and invasin Als3 of Candida albicans mediates iron acquisition from host ferritin","volume":"4","author":"RS Almeida","year":"2008","journal-title":"PLOS Pathog"},{"key":"pone.0261180.ref041","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/EC.00279-10","article-title":"Candida albicans Als3, a multifunctional adhesin and invasin","volume":"10","author":"Y Liu","year":"2011","journal-title":"Eukaryot Cell"},{"key":"pone.0261180.ref042","first-page":"139","article-title":"Green Clayton B, Yeater Kathleen M, Soll David R. LLH. Candida albicans Als3p is required for wild-type biofilm formation on silicone elastomer surfaces","volume":"176","author":"Zhao Xiaomin","year":"2019","journal-title":"Physiol Behav"},{"issue":"14","key":"pone.0261180.ref043","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.cub.2008.06.034","article-title":"Complementary adhesin function in C. albicans biofilm formation","volume":"18","author":"CJ Nobile","year":"2008","journal-title":"Curr Biol"},{"key":"pone.0261180.ref044","first-page":"4","article-title":"Candida albicans hyphae: From growth initiation to invasion","author":"JV Desai","year":"2018","journal-title":"J Fungi (Basel)"},{"key":"pone.0261180.ref045","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/1471-2180-13-246","article-title":"C. albicans growth, transition, biofilm formation, and gene expression modulation by antimicrobial decapeptide KSL-W","volume":"13","author":"S Theberge","year":"2013","journal-title":"BMC Microbiol"},{"key":"pone.0261180.ref046","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1128\/EC.00340-06","article-title":"Developmental regulation of an adhesin gene during cellular morphogenesis in the fungal pathogen Candida albicans","volume":"6","author":"S Argim\u00f3n","year":"2007","journal-title":"Eukaryot Cell"},{"key":"pone.0261180.ref047","volume-title":"Clin Oral Surgery","author":"A. Filippi","year":"2001"},{"issue":"5","key":"pone.0261180.ref048","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3892\/br.2014.290","article-title":"Anti-inflammatory effects of ozonated water in an experimental mouse model","volume":"2","author":"A Kazuo","year":"2014","journal-title":"Biomed Rep"},{"key":"pone.0261180.ref049","doi-asserted-by":"crossref","first-page":"579","DOI":"10.21873\/invivo.11097","article-title":"Microbicidal Effects of Stored Aqueous Ozone Solution Generated by Nano-bubble Technology","volume":"31","author":"S Mineaki","year":"2017","journal-title":"In Vivo"},{"key":"pone.0261180.ref050","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/j.1365-2621.2000.tb16040.x","article-title":"Inactivation kinetics of foodborne spoilage and pathogenic bacteria by ozone","volume":"65","author":"J-G Kim","year":"2000","journal-title":"J Food Sci"},{"key":"pone.0261180.ref051","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1111\/j.1365-2621.2001.tb15196.x","article-title":"Microbiological aspects of ozone applications in food: A review","volume":"66","author":"MA Khadre","year":"2001","journal-title":"J Food Sci"},{"key":"pone.0261180.ref052","doi-asserted-by":"crossref","first-page":"155","DOI":"10.4265\/bio.24.155","article-title":"Investigation of the effective concentration of ozonated water for disinfection in the presence of protein contaminants","volume":"24","author":"K Hirai","year":"2019","journal-title":"Biocontrol Sci."},{"issue":"2","key":"pone.0261180.ref053","doi-asserted-by":"crossref","first-page":"220","DOI":"10.4012\/dmj.15.220","article-title":"Disinfection of removable dentures using ozone","volume":"15","author":"H Murakami","year":"1996","journal-title":"Dent Mater J"},{"key":"pone.0261180.ref054","doi-asserted-by":"crossref","first-page":"24663","DOI":"10.1039\/C6CP05064F","article-title":"Coote. 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