{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:51:03Z","timestamp":1772211063163,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T00:00:00Z","timestamp":1639612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007224","name":"National Foundation for Science and Technology Development","doi-asserted-by":"publisher","award":["108.06-2017.21"],"award-info":[{"award-number":["108.06-2017.21"]}],"id":[{"id":"10.13039\/100007224","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Early diagnosis, precise antimicrobial treatment and subsequent patient stratification can improve sepsis outcomes. Circulating biomarkers such as plasma microRNAs (miRNAs) have proven to be surrogates for diagnosis, severity and case management of infections. The expression of four selected miRNAs (miR-146-3p, miR-147b, miR-155 and miR-223) was validated for their prognostic and diagnostic potential in a clinically defined cohort of patients with sepsis and septic shock.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>The expression of plasma miRNAs was quantified by quantitative PCR (qPCR) in patients with bacterial sepsis (n = 78), in patients with septic shock (n = 52) and in patients with dengue haemorrhagic fever (DHF; n = 69) and in healthy controls (n = 82).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>The expression of studied miRNA was significantly increased in patients with bacterial sepsis and septic shock. The plasma miR-147b was able to differentiate bacterial sepsis from non-sepsis and septic shock (AUC = 0.77 and 0.8, respectively, p\u2264 0.05), while the combination of plasma miR-147b and procalcitonin (PCT) predicted septic shock (AUC = 0.86, p\u2264 0.05).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The plasma miR-147b may be an useful biomarker independently or in combination with PCT to support clinical diagnosis of sepsis and equally prognosis of patients with septic shock.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0261228","type":"journal-article","created":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T18:47:05Z","timestamp":1639680425000},"page":"e0261228","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Circulating miR-147b as a diagnostic marker for patients with bacterial sepsis and septic shock"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9808-1176","authenticated-orcid":true,"given":"Ngo Tat","family":"Trung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tran Thi","family":"Lien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vu Viet","family":"Sang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nghiem Xuan","family":"Hoan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Dang","family":"Manh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Sy","family":"Thau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dao Thanh","family":"Quyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tran Thi Thu","family":"Hien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phan Quoc","family":"Hoan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai Hong","family":"Bang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thirumalaisamy P.","family":"Velavan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le Huu","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,12,16]]},"reference":[{"key":"pone.0261228.ref001","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3)","volume":"315","author":"Mervyn Singer CSD, Christopher Warren Seymour","year":"2016","journal-title":"JAMA"},{"key":"pone.0261228.ref002","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1186\/s13054-018-2157-z","article-title":"The global burden of sepsis: barriers and potential solutions","volume":"22","author":"KE Rudd","year":"2018","journal-title":"Crit Care"},{"key":"pone.0261228.ref003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1556\/EuJMI.4.2014.1.1","article-title":"Molecular diagnosis of sepsis: New aspects and recent developments","volume":"4","author":"L Oliver Liesenfeld","year":"2014","journal-title":"Eur J Microbiol Immunol (Bp)"},{"key":"pone.0261228.ref004","doi-asserted-by":"crossref","first-page":"13663","DOI":"10.1038\/s41598-019-50150-y","article-title":"PCR-based Sepsis@Quick test is superior in comparison with blood culture for identification of sepsis-causative pathogens","volume":"9","author":"NT Trung","year":"2019","journal-title":"Sci Rep"},{"key":"pone.0261228.ref005","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4037\/ajcc2016728","article-title":"Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures","volume":"25","author":"B Suberviola","year":"2016","journal-title":"Am J Crit Care"},{"key":"pone.0261228.ref006","article-title":"Epidemiology and Changes in Mortality of Sepsis After the Implementation of Surviving Sepsis Campaign Guidelines","author":"R Herran-Monge","year":"2017","journal-title":"J Intensive Care Med"},{"key":"pone.0261228.ref007","first-page":"499","article-title":"The Use of Biomarkers in Sepsis","volume":"18","author":"K Giannakopoulos","year":"2017","journal-title":"A Systematic Review. Curr Pharm Biotechnol"},{"key":"pone.0261228.ref008","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3947\/ic.2020.52.1.1","article-title":"An Update on Sepsis Biomarkers","volume":"52","author":"MH Kim","year":"2020","journal-title":"Infect Chemother"},{"key":"pone.0261228.ref009","doi-asserted-by":"crossref","DOI":"10.3390\/ijms17010078","article-title":"Circulating MicroRNAs as Biomarkers for Sepsis","volume":"17","author":"F Benz","year":"2016","journal-title":"Int J Mol Sci"},{"key":"pone.0261228.ref010","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1146\/annurev-immunol-020711-075013","article-title":"microRNA regulation of inflammatory responses","volume":"30","author":"RM O\u2019Connell","year":"2012","journal-title":"Annu Rev Immunol"},{"key":"pone.0261228.ref011","doi-asserted-by":"crossref","first-page":"15819","DOI":"10.1073\/pnas.0901216106","article-title":"miR-147, a microRNA that is induced upon Toll-like receptor stimulation, regulates murine macrophage inflammatory responses","volume":"106","author":"G Liu","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0261228.ref012","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1073\/pnas.0610731104","article-title":"MicroRNA-155 is induced during the macrophage inflammatory response","volume":"104","author":"RM O\u2019Connell","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0261228.ref013","doi-asserted-by":"crossref","first-page":"e54612","DOI":"10.1371\/journal.pone.0054612","article-title":"Circulating microRNA-150 serum levels predict survival in patients with critical illness and sepsis","volume":"8","author":"C Roderburg","year":"2013","journal-title":"PLoS One"},{"key":"pone.0261228.ref014","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.bbrc.2010.02.145","article-title":"Serum miR-146a and miR-223 as potential new biomarkers for sepsis","volume":"394","author":"JF Wang","year":"2010","journal-title":"Biochem Biophys Res Commun"},{"key":"pone.0261228.ref015","doi-asserted-by":"crossref","first-page":"e46551","DOI":"10.1371\/journal.pone.0046551","article-title":"Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10","volume":"7","author":"J Zhu","year":"2012","journal-title":"PLoS One"},{"key":"pone.0261228.ref016","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s40560-020-00497-6","article-title":"Accuracy of circulating microRNAs in diagnosis of sepsis: a systematic review and meta-analysis","volume":"8","author":"X Shen","year":"2020","journal-title":"J Intensive Care"},{"key":"pone.0261228.ref017","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/nature06607","article-title":"Regulation of progenitor cell proliferation and granulocyte function by microRNA-223","volume":"451","author":"JB Johnnidis","year":"2008","journal-title":"Nature"},{"key":"pone.0261228.ref018","doi-asserted-by":"crossref","first-page":"e179","DOI":"10.1093\/nar\/gni178","article-title":"Real-time quantification of microRNAs by stem-loop RT-PCR","volume":"33","author":"C Chen","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"pone.0261228.ref019","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"ST Livak KJ","year":"2001","journal-title":"Methods"},{"key":"pone.0261228.ref020","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1261\/rna.939908","article-title":"Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues","volume":"14","author":"HJ Peltier","year":"2008","journal-title":"RNA"},{"key":"pone.0261228.ref021","author":"A Kumar","journal-title":"Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock"},{"key":"pone.0261228.ref022","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.burns.2008.01.014","article-title":"Comparison of WBC, ESR, CRP and PCT serum levels in septic and non-septic burn cases","volume":"34","author":"M Barati","year":"2008","journal-title":"Burns"},{"key":"pone.0261228.ref023","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S1473-3099(07)70052-X","article-title":"Accuracy of procalcitonin for sepsis diagnosis in critically ill patients: systematic review and meta-analysis","volume":"7","author":"BMP Tang","year":"2007","journal-title":"The Lancet Infectious Diseases"},{"key":"pone.0261228.ref024","first-page":"142","article-title":"SSC International Guideline 2016\u2014Management of Sepsis and Septic Shock","volume":"53","author":"T Rahmel","year":"2018","journal-title":"Anasthesiol Intensivmed Notfallmed Schmerzther"},{"key":"pone.0261228.ref025","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1111\/cmi.12159","article-title":"MicroRNAs and bacterial infection","volume":"15","author":"C Staedel","year":"2013","journal-title":"Cell Microbiol"},{"key":"pone.0261228.ref026","doi-asserted-by":"crossref","first-page":"L622","DOI":"10.1152\/ajplung.00477.2005","article-title":"NF-kappa B activation as a pathological mechanism of septic shock and inflammation","volume":"290","author":"SF Liu","year":"2006","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"pone.0261228.ref027","doi-asserted-by":"crossref","first-page":"658524","DOI":"10.3389\/fphys.2021.658524","article-title":"Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions.","volume":"12","author":"B Szilagyi","year":"2021","journal-title":"Front Physiol"},{"key":"pone.0261228.ref028","first-page":"1178631018797079","article-title":"Expression Levels of Candidate Circulating microRNAs in Early-Onset Neonatal Sepsis Compared With Healthy Newborns","volume":"11","author":"BB Dhas","year":"2018","journal-title":"Genomics Insights"},{"key":"pone.0261228.ref029","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.3892\/etm.2013.937","article-title":"Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients","volume":"5","author":"L Wang","year":"2013","journal-title":"Exp Ther Med"},{"key":"pone.0261228.ref030","doi-asserted-by":"crossref","first-page":"e110286","DOI":"10.1371\/journal.pone.0110286","article-title":"MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression","volume":"9","author":"V Chatterjee","year":"2014","journal-title":"PLoS One"},{"key":"pone.0261228.ref031","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1097\/00003246-199806000-00019","article-title":"Septic shock: an analysis of outcomes for patients with onset on hospital wards versus intensive care units","volume":"26","author":"JS Lundberg","year":"1998","journal-title":"Crit Care Med"},{"key":"pone.0261228.ref032","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijid.2015.09.016","article-title":"Elevated miR-155 expression induces immunosuppression via CD39(+) regulatory T-cells in sepsis patient","volume":"40","author":"J Liu","year":"2015","journal-title":"Int J Infect Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0261228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T18:47:36Z","timestamp":1639680456000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0261228"}},"subtitle":[],"editor":[{"given":"Colin","family":"Johnson","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,12,16]]},"references-count":32,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0261228","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,16]]}}}