{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:15:01Z","timestamp":1747196101923,"version":"3.40.5"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016730","name":"Torii Pharmaceutical Co Ltd","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016730","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Japanese cedar pollinosis is a major seasonal allergy in Japan, and Japanese cypress pollinosis is a growing concern because the cypress pollen season follows the cedar pollen season and cross-reactivity among allergens occurs between these closely related species. Allergens purified from pollen under unspecified collecting conditions can potentially heterogenous allergens profiles and batch to batch variability, and amino acid sequence variants in allergens possibly exist among trees. Polymorphisms have not been investigated for the cypress pollen major allergens, Cha o 1 and Cha o 2. Our aim was to examine the homogeneity of allergen amino acid sequences. DNA sequences of Cha o 1 and Cha o 2 from pollen collected from Chiba and Ibaraki prefectures and from needles of 47 plus trees located at seed orchards in Chiba Prefecture were examined by amplicon sequencing and amino acid substitutions were deduced. Sequence analysis of the pollen samples revealed that eight and seven residues of Cha o 2 were polymorphic, respectively. Thirteen residues in Cha o 2, including those residues identified in pollen, were deduced to be polymorphic for the plus trees. Cha o 2 expressed by the 47 plus trees included amino acid differences when compared with that of isoallergen Cha o 2.0101. No substitution was deduced in Cha o 1 for pollen taken from the two prefectures. One conservative amino acid substitution was deduced in Cha o 1 for the plus trees. Of the 47 plus trees examined, 38 were deduced to express only the isoallergen Cha o 1.0101 isoform, whereas eight trees were heterozygous and a single tree was homozygous for the non-synonymous mutation, which indicates relative uniformity of Cha o 1. Cha o 2 was found to be a heterogeneous allergen which suggests that studies using pollen from different trees may not give the same results.<\/jats:p>","DOI":"10.1371\/journal.pone.0261327","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T19:39:43Z","timestamp":1640720383000},"page":"e0261327","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polymorphisms in Cha o 1 and Cha o 2, major allergens of Japanese cypress (Chamaecyparis obtusa) pollen from a restricted region in Japan"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2574-2919","authenticated-orcid":true,"given":"Minoru","family":"Tateno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaka","family":"Enami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Fujinami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,12,28]]},"reference":[{"issue":"3","key":"pone.0261327.ref001","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/j.1398-9995.2005.00731.x","article-title":"Allergy to cypress pollen","volume":"60","author":"D Charpin","year":"2005","journal-title":"Allergy"},{"issue":"6","key":"pone.0261327.ref002","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/j.1365-2222.2010.03470.x","article-title":"Cross-sectional survey on immunoglobulin E reactivity in 23,077 subjects using an allergenic molecule-based microarray detection system","volume":"40","author":"E Scala","year":"2010","journal-title":"Clin Exp Allergy"},{"issue":"10","key":"pone.0261327.ref003","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1111\/all.12696","article-title":"Tree pollen allergens-an update from a molecular perspective","volume":"70","author":"C Asam","year":"2015","journal-title":"Allergy"},{"issue":"6","key":"pone.0261327.ref004","doi-asserted-by":"crossref","first-page":"203","DOI":"10.2183\/pjab.90.203","article-title":"Japanese cedar pollinosis: discovery, nomenclature, and epidemiological trends","volume":"90","author":"Y. 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Int."},{"issue":"11","key":"pone.0261327.ref020","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1111\/j.1365-2222.2004.02108.x","article-title":"Cry j 1 isoforms derived from Cryptomeria japonica trees have different binding properties to monoclonal antibodies","volume":"34","author":"Y Goto","year":"2004","journal-title":"Clin Exp Allergy"},{"issue":"6","key":"pone.0261327.ref021","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1016\/j.jaip.2018.08.032","article-title":"Allergen Extracts for In Vivo Diagnosis and Treatment of Allergy: Is There a Future?","volume":"6","author":"R Valenta","year":"2018","journal-title":"J Allergy Clin Immunol Pract"},{"issue":"2","key":"pone.0261327.ref022","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s00239-008-9140-2","article-title":"Adaptive and slightly deleterious evolution in a conifer, Cryptomeria japonica","volume":"67","author":"A Fujimoto","year":"2008","journal-title":"J Mol Evol"},{"issue":"4","key":"pone.0261327.ref023","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/jof\/96.4.12","article-title":"Japanese Tree Improvement and Forest Genetics","volume":"96","author":"S McKeand","year":"1998","journal-title":"Journal of Forestry"},{"issue":"4","key":"pone.0261327.ref024","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/all.12348","article-title":"Update of the WHO\/IUIS Allergen Nomenclature Database based on analysis of allergen sequences","volume":"69","author":"C Radauer","year":"2014","journal-title":"Allergy"},{"issue":"12","key":"pone.0261327.ref025","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1074\/mcp.M112.020800","article-title":"High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays","volume":"11","author":"S Buus","year":"2012","journal-title":"Mol Cell 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antagonism","volume":"97","author":"S Ikagawa","year":"1996","journal-title":"J Allergy Clin Immunol"},{"issue":"2","key":"pone.0261327.ref029","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1046\/j.1365-2222.2003.01579.x","article-title":"Analysis of sequential immunoglobulin E-binding epitope of Japanese cedar pollen allergen (Cry j 2) in humans, monkeys and mice","volume":"33","author":"Y Tamura","year":"2003","journal-title":"Clin Exp Allergy"},{"issue":"5","key":"pone.0261327.ref030","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s10875-013-9880-7","article-title":"Analysis of conformational and sequential IgE epitopes on the major allergen Cry j 2 of Japanese cedar (Cryptomeria japonica) pollen in humans by using monoclonal antibodies for Cry j 2","volume":"33","author":"K Miyaji","year":"2013","journal-title":"J Clin 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