{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T11:38:47Z","timestamp":1778067527587,"version":"3.51.4"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T00:00:00Z","timestamp":1639526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004386","name":"universiti malaya","doi-asserted-by":"publisher","award":["RP013B-13HTM"],"award-info":[{"award-number":["RP013B-13HTM"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"universiti malaya","doi-asserted-by":"publisher","award":["FRGS\/1\/2020\/SKK0\/UM\/02\/7 (FP018-2020)"],"award-info":[{"award-number":["FRGS\/1\/2020\/SKK0\/UM\/02\/7 (FP018-2020)"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic> (<jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic>) is an intracellular pathogen that causes melioidosis, a life-threatening infection in humans. The bacterium is able to form small colony variants (SCVs) as part of the adaptive features in response to environmental stress. In this study, we characterize the genomic characteristics, antimicrobial resistance (AMR), and metabolic phenotypes of <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> SCV and wild type (WT) strains. Whole-genome sequence analysis was performed to characterize the genomic features of two SCVs (CS and OS) and their respective parental WT strains (CB and OB). Phylogenetic relationship between the four draft genomes in this study and 19 publicly available genomes from various countries was determined. The four draft genomes showed a close phylogenetic relationship with other genomes from Southeast Asia. Broth microdilution and phenotype microarray were conducted to determine the AMR profiles and metabolic features (carbon utilization, osmolytes sensitivity, and pH conditions) of all strains. The SCV strains exhibited identical AMR phenotype with their parental WT strains. A limited number of AMR-conferring genes were identified in the <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> genomes. The SCVs and their respective parental WT strains generally shared similar carbon-utilization profiles, except for D,L-carnitine (CS), g-hydroxybutyric acid (OS), and succinamic acid (OS) which were utilized by the SCVs only. No difference was observed in the osmolytes sensitivity of all strains. In comparison, WT strains were more resistant to alkaline condition, while SCVs showed variable growth responses at higher acidity. Overall, the genomes of the colony morphology variants of <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> were largely identical, and the phenotypic variations observed among the different morphotypes were strain-specific.<\/jats:p>","DOI":"10.1371\/journal.pone.0261382","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T18:36:00Z","timestamp":1639593360000},"page":"e0261382","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genomic comparison and phenotypic profiling of small colony variants of Burkholderia pseudomallei"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9124-8826","authenticated-orcid":true,"given":"Noorfatin 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