{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T21:05:34Z","timestamp":1784754334950,"version":"3.55.0"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T00:00:00Z","timestamp":1647561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"Narodowym Centrum Nauki","doi-asserted-by":"publisher","award":["NCN 2016\/21\/B\/NZ4\/00131"],"award-info":[{"award-number":["NCN 2016\/21\/B\/NZ4\/00131"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Anhydrobiosis is considered to be an adaptation of important applicative implications because it enables resistance to the lack of water. The phenomenon is still not well understood at molecular level. Thus, a good model invertebrate species for the research is required. The best known anhydrobiotic invertebrates are tardigrades (Tardigrada), considered to be toughest animals in the world.\n                    <jats:italic>Hypsibius<\/jats:italic>\n                    .\n                    <jats:italic>exemplaris<\/jats:italic>\n                    is one of the best studied tardigrade species, with its name \u201c\n                    <jats:italic>exemplaris<\/jats:italic>\n                    \u201d referring to the widespread use of the species as a laboratory model for various types of research. However, available data suggest that anhydrobiotic capability of the species may be overestimated. Therefore, we determined anhydrobiosis survival by\n                    <jats:italic>Hys<\/jats:italic>\n                    .\n                    <jats:italic>exemplaris<\/jats:italic>\n                    specimens using three different anhydrobiosis protocols. We also checked ultrastructure of storage cells within formed dormant structures (tuns) that has not been studied yet for\n                    <jats:italic>Hys<\/jats:italic>\n                    .\n                    <jats:italic>exemplaris<\/jats:italic>\n                    . These cells are known to support energetic requirements of anhydrobiosis. The obtained results indicate that\n                    <jats:italic>Hys<\/jats:italic>\n                    .\n                    <jats:italic>exemplaris<\/jats:italic>\n                    appears not to be a good model species for anhydrobiosis research.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0261485","type":"journal-article","created":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T13:29:06Z","timestamp":1647610146000},"page":"e0261485","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Verification of Hypsibius exemplaris G\u0105siorek et al., 2018 (Eutardigrada; Hypsibiidae) application in anhydrobiosis research"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8930-1592","authenticated-orcid":true,"given":"Izabela","family":"Poprawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-6529","authenticated-orcid":true,"given":"Tomasz","family":"Bartylak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Kulpla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weronika","family":"Erdmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Roszkowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0141ukasz","family":"Chajec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5260-6253","authenticated-orcid":true,"given":"\u0141ukasz","family":"Kaczmarek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andonis","family":"Karachitos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Kmita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,3,18]]},"reference":[{"key":"pone.0261485.ref001","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1146\/annurev.ph.54.030192.003051","article-title":"Anhydrobiosis","volume":"54","author":"JH Crowe","year":"1992","journal-title":"Ann Rev Physiol"},{"issue":"1","key":"pone.0261485.ref002","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1303\/aez.2006.15","article-title":"Anhydrobiosis in invertebrates","volume":"41","author":"M. 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Garc\u00eda","year":"2011","journal-title":"J Biosci"},{"issue":"1","key":"pone.0261485.ref017","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.biotechadv.2018.11.003","article-title":"Anhydrobiosis: Inside yeast cells","volume":"37","author":"A Rapoport","year":"2019","journal-title":"Biotechnol Adv"},{"key":"pone.0261485.ref018","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1242\/jeb.205.18.2799","article-title":"Mechanism allowing an insect to survive complete dehydration and extreme temperatures","author":"M Watanabe","year":"2002","journal-title":"J Exp Biol"},{"key":"pone.0261485.ref019","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/978-3-319-95702-9_10","volume-title":"Water bears: The biology of tardigrades","author":"RO Schill","year":"2018"},{"issue":"Suppl","key":"pone.0261485.ref020","first-page":"37","article-title":"The physiology of cryptobiosis in tardigrades","volume":"32","author":"JH Crowe","year":"1975","journal-title":"Mem Ist Ital Idrobiol"},{"issue":"1","key":"pone.0261485.ref021","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1469-185X.1992.tb01657.x","article-title":"Cryptobiosis in Tardigrada","volume":"67","author":"JC Wright","year":"1992","journal-title":"Biol Rev"},{"issue":"12","key":"pone.0261485.ref022","doi-asserted-by":"crossref","first-page":"e85091","DOI":"10.1371\/journal.pone.0085091","article-title":"Desiccation tolerance in the tardigrade Richtersius coronifer relies on muscle mediated structural reorganization","volume":"8","author":"KA Halberg","year":"2013","journal-title":"PLOS ONE"},{"key":"pone.0261485.ref023","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/B978-0-12-385026-3.00017-6","volume-title":"Ecology and general biology: Thorp and Covich\u2019s Freshwater invertebrates","author":"DR Nelson","year":"2015"},{"issue":"3","key":"pone.0261485.ref024","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1007\/s00709-016-1027-2","article-title":"The structure of the desiccated Richtersius coronifer (Richters, 1903)","volume":"254","author":"M Czernekov\u00e1","year":"2017","journal-title":"Protoplasma"},{"issue":"8","key":"pone.0261485.ref025","doi-asserted-by":"crossref","first-page":"e0201430","DOI":"10.1371\/journal.pone.0201430","article-title":"A comparative ultrastructure study of storage cells in the eutardigrade Richtersius coronifer in the hydrated state and after desiccation and heating stress","volume":"13","author":"M Czernekov\u00e1","year":"2018","journal-title":"PLOS ONE"},{"key":"pone.0261485.ref026","doi-asserted-by":"crossref","first-page":"110890","DOI":"10.1016\/j.cbpa.2020.110890","article-title":"New insights into survival strategies of tardigrades","volume":"254","author":"N M\u00f8bjerg","year":"2020","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"issue":"1","key":"pone.0261485.ref027","doi-asserted-by":"crossref","first-page":"45","DOI":"10.11646\/zootaxa.4415.1.2","article-title":"An integrative redescription of Hypsibius dujardini (Doy\u00e8re, 1840), the nominal taxon for Hypsibioidea (Tardigrada: Eutardigrada)","volume":"4415","author":"P G\u0105siorek","year":"2018","journal-title":"Zootaxa"},{"key":"pone.0261485.ref028","first-page":"269","article-title":"Memoire sur les Tardigrades. I","volume":"14","author":"LMF Doy\u00e8re","year":"1840","journal-title":"Annls Sci Nat Paris"},{"key":"pone.0261485.ref029","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/j.1096-3642.1993.tb02538.x","article-title":"A new species of Ramazzottius (Tardigrada, Hypsibiidae) in a rain gutter sediment from England","volume":"109","author":"R Bertolani","year":"1993","journal-title":"Zool J Linn Soc"},{"issue":"18","key":"pone.0261485.ref030","doi-asserted-by":"crossref","first-page":"5053","DOI":"10.1073\/pnas.1600338113","article-title":"No evidence for extensive horizontal gene transfer in the genome of the tardigrade Hypsibius dujardini","volume":"113","author":"G Koutsovoulos","year":"2016","journal-title":"PNAS"},{"key":"pone.0261485.ref031","doi-asserted-by":"crossref","first-page":"160063","DOI":"10.1038\/sdata.2016.63","article-title":"Genome sequencing of a single tardigrade Hypsibius dujardini individual","volume":"3","author":"K Arakawa","year":"2016","journal-title":"Sci Data"},{"key":"pone.0261485.ref032","doi-asserted-by":"crossref","first-page":"12808","DOI":"10.1038\/ncomms12808","article-title":"Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein","volume":"7","author":"T Hashimoto","year":"2016","journal-title":"Nat Commun"},{"key":"pone.0261485.ref033","unstructured":"Degma P, Bertolani R, Guidetti R. Actual checklist of Tardigrada species (2009\u20132021, 40th Edition: 19-07-2021). 2009\u20132021; https:\/\/iris.unimore.it\/retrieve\/358743\/Actual%20checklist%20of%20Tardigrada%2040th%20Edition%2019-07-21.pdf."},{"key":"pone.0261485.ref034","doi-asserted-by":"crossref","first-page":"592016","DOI":"10.3389\/fphys.2020.592016","article-title":"Mechanisms of desiccation tolerance: themes and variations in brine shrimp, roundworms, and tardigrades","volume":"11","author":"JD Hibshman","year":"2020","journal-title":"Front Physiol"},{"issue":"1","key":"pone.0261485.ref035","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1186\/s12964-020-00670-2","article-title":"The biology of tardigrade disordered proteins in extreme stress tolerance","volume":"18","author":"C Hesgrove","year":"2020","journal-title":"Cell Commun Signal"},{"issue":"1","key":"pone.0261485.ref036","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1186\/s12864-019-5912-x","article-title":"Comparative transcriptomics suggest unique molecular adaptations within tardigrade lineages","volume":"20","author":"M Kamilari","year":"2019","journal-title":"BMC Genomics"},{"issue":"12","key":"pone.0261485.ref037","doi-asserted-by":"crossref","first-page":"e0144803","DOI":"10.1371\/journal.pone.0144803","article-title":"Suggested involvement of PP1\/PP2A activity and de novo gene expression in anhydrobiotic survival in a tardigrade, Hypsibius dujardini, by chemical genetic approach","volume":"10","author":"K Kondo","year":"2015","journal-title":"PLOS ONE"},{"issue":"22","key":"pone.0261485.ref038","doi-asserted-by":"crossref","first-page":"E3054","DOI":"10.1073\/pnas.1525116113","article-title":"Genome of a tardigrade: Horizontal gene transfer or bacterial contamination?","volume":"113","author":"F Bemm","year":"2016","journal-title":"PNAS"},{"issue":"7","key":"pone.0261485.ref039","doi-asserted-by":"crossref","first-page":"e2002266","DOI":"10.1371\/journal.pbio.2002266","article-title":"Comparative genomics of the tardigrades Hypsibius dujardini and Ramazzottius varieornatus","volume":"15","author":"Y Yoshida","year":"2017","journal-title":"PLOS Biol"},{"issue":"9","key":"pone.0261485.ref040","doi-asserted-by":"crossref","first-page":"e0183380","DOI":"10.1371\/journal.pone.0183380","article-title":"Can the tardigrade Hypsibius dujardini survive in the absence of the geomagnetic field?","volume":"12","author":"W Erdmann","year":"2017","journal-title":"PLOS ONE"},{"key":"pone.0261485.ref041","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1111\/zoj.12467","article-title":"Ultrastructural changes in the midgut epithelium of Hypsibius dujardini (Doy\u00e8re, 1840) (Tardigrada, Eutardigrada, Hypsibiidae) in relation to oogenesis","volume":"178","author":"M Hyra","year":"2016a","journal-title":"Zool J Linn Soc"},{"issue":"4","key":"pone.0261485.ref042","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1111\/zoj.12463","article-title":"Body cavity cells of Parachela during their active life","volume":"178","author":"M Hyra","year":"2016b","journal-title":"Zool J Linn Soc"},{"issue":"2","key":"pone.0261485.ref043","first-page":"65","article-title":"Life history traits for the freshwater tardigrade species Hypsibius exemplaris reared under laboratory conditions","volume":"4","author":"J Stone","year":"2020","journal-title":"JWB"},{"issue":"1\/3","key":"pone.0261485.ref044","doi-asserted-by":"crossref","first-page":"103126","DOI":"10.1016\/j.micron.2021.103126","article-title":"Oogenesis in the tardigrade Hypsibius exemplaris G\u0105siorek, Stec, Morek & Michalczyk, 2018 (Eutardigrada, Hypsibiidae)","volume":"150","author":"M Jezierska","year":"2021","journal-title":"Micron"},{"key":"pone.0261485.ref045","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1242\/jeb.142.1.267","article-title":"Desiccation tolerance and water-retentive mechanisms in tardigrades","volume":"142","author":"JC Wright","year":"1989","journal-title":"J Exp Biol"},{"issue":"12","key":"pone.0261485.ref046","doi-asserted-by":"crossref","first-page":"e0208617","DOI":"10.1371\/journal.pone.0208617","article-title":"Carbonylation accumulation of the Hypsibius exemplaris anhydrobiote reveals age-associated marks","volume":"13","author":"M Kuzmic","year":"2018","journal-title":"PLOS ONE"},{"issue":"1","key":"pone.0261485.ref047","doi-asserted-by":"crossref","first-page":"4324","DOI":"10.1038\/s41598-020-61165-1","article-title":"Ultrastructural analysis of the dehydrated tardigrade Hypsibius exemplaris unveils an anhydrobiotic-specific architecture","volume":"10","author":"M Richaud","year":"2020","journal-title":"Sci 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