{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:15:16Z","timestamp":1747196116515,"version":"3.40.5"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>Parotid cancer is relatively rare, and malignancy varies; therefore, novel markers are needed to predict prognosis. Recent advances in matrix-assisted laser desorption\/ionization imaging mass spectrometry (MALDI-IMS), useful for visualization of lipid molecules, have revealed the relationship between cancer and lipid metabolism, indicating the potential of lipids as biomarkers. However, the distribution and importance of phospholipids in parotid cancer remain unclear.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Objective<\/jats:title><jats:p>This study aimed to use MALDI-IMS to comprehensively investigate the spatial distribution of phospholipids characteristically expressed in human parotid cancer tissues.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Methods<\/jats:title><jats:p>Tissue samples were surgically collected from two patients with parotid cancer (acinic cell carcinoma and mucoepidermoid carcinoma). Frozen sections of the samples were assessed using MALDI-IMS in both positive and negative ion modes, with an<jats:italic>m\/z<\/jats:italic>range of 600\u20131000. The mass spectra obtained in the tumor and non-tumor regions were compared and analyzed. Ion images corresponding to the peak characteristics of the tumor regions were visualized.<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Results<\/jats:title><jats:p>Several candidate phospholipids with significantly different expression levels were detected between the tumor and non-tumor regions. The number of unique lipid peaks with significantly different intensities between the tumor and non-tumor regions was 95 and 85 for Cases 1 and 2, respectively, in positive ion mode, and 99 and 97 for Cases 1 and 2, respectively, in negative ion mode. Imaging differentiated the characteristics that phospholipids were heterogeneously distributed in the tumor regions.<\/jats:p><\/jats:sec><jats:sec id=\"sec005\"><jats:title>Conclusion<\/jats:title><jats:p>Phospholipid candidates that are characteristically expressed in human parotid cancer tissues were found, demonstrating the localization of their expression. These findings are notable for further investigation of alterations in lipid metabolism of parotid cancer and may have potential for the development of phospholipids as biomarkers.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pone.0261491","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T18:39:40Z","timestamp":1639766380000},"page":"e0261491","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Short communication: Distribution of phospholipids in parotid cancer by matrix-assisted laser desorption\/ionization imaging mass 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El-Naggar","year":"2017","edition":"4"},{"key":"pone.0261491.ref002","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s12105-017-0795-0","article-title":"Update from the 4th Edition of the World Health Organization Classification of Head and Neck Tumours: Tumors of the Salivary Gland.","volume":"11","author":"RR Seethala","year":"2017","journal-title":"Head Neck Pathol"},{"key":"pone.0261491.ref003","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1007\/s00405-020-05871-6","article-title":"Challenges with preoperative diagnosis of low\/intermediate-grade carcinoma of the parotid gland: single-center study of 112 patients.","volume":"277","author":"S Omura","year":"2020","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"pone.0261491.ref004","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1007\/s00405-019-05604-4","article-title":"Clinical characteristics of acinic cell carcinoma and secretory carcinoma of the parotid gland","volume":"276","author":"T Terada","year":"2019","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"pone.0261491.ref005","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1080\/00016489.2019.1683604","article-title":"Programmed death ligand-1 expression is associated with stage and histological grade of parotid carcinoma.","volume":"140","author":"M Higashino","year":"2020","journal-title":"Acta Otolaryngol"},{"key":"pone.0261491.ref006","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s00508-020-01778-8","article-title":"Molecularly guided treatment of metastatic parotid gland carcinoma in adults.","volume":"133","author":"H Taghizadeh","year":"2021","journal-title":"Wien Klin Wochenschr"},{"key":"pone.0261491.ref007","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.cbpa.2009.08.011","article-title":"Lipidomics: a mass spectrometry based systems level analysis of cellular lipids","volume":"13","author":"PT Ivanova","year":"2009","journal-title":"Curr Opin Chem Biol"},{"key":"pone.0261491.ref008","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.addr.2020.07.013","article-title":"Lipids and cancer: Emerging roles in pathogenesis, diagnosis and therapeutic intervention","volume":"159","author":"LM Butler","year":"2020","journal-title":"Adv Drug Deliv Rev"},{"key":"pone.0261491.ref009","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/febs.14281","article-title":"Aberrant lipid metabolism in cancer cells\u2014the role of oncolipid-activated signaling","volume":"285","author":"U Ray","year":"2018","journal-title":"Febs J"},{"key":"pone.0261491.ref010","article-title":"Role of Aberrant Lipid Metabolism of Cancer Stem Cells in Cancer Progression.","author":"J Zhou","year":"2021","journal-title":"Curr Cancer Drug Targets"},{"key":"pone.0261491.ref011","doi-asserted-by":"crossref","DOI":"10.3390\/cancers13030474","article-title":"Lipid Metabolism in Oncology: Why It Matters, How to Research, and How to Treat.","volume":"13","author":"Y Matsushita","year":"2021","journal-title":"Cancers (Basel)."},{"key":"pone.0261491.ref012","doi-asserted-by":"crossref","first-page":"3818","DOI":"10.1021\/bi301519p","article-title":"Matrix-assisted laser desorption ionization imaging mass spectrometry: in situ molecular mapping","volume":"52","author":"PM Angel","year":"2013","journal-title":"Biochemistry"},{"key":"pone.0261491.ref013","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.tibtech.2010.12.002","article-title":"MALDI imaging mass spectrometry of human tissue: method challenges and clinical perspectives","volume":"29","author":"EH Seeley","year":"2011","journal-title":"Trends Biotechnol"},{"key":"pone.0261491.ref014","doi-asserted-by":"crossref","first-page":"3237","DOI":"10.1021\/cr100012c","article-title":"Mass spectrometric imaging for biomedical tissue analysis","volume":"110","author":"K Chughtai","year":"2010","journal-title":"Chem Rev"},{"key":"pone.0261491.ref015","doi-asserted-by":"crossref","first-page":"e1229","DOI":"10.1002\/cnr2.1229","article-title":"Mass spectrometry imaging to detect lipid biomarkers and disease signatures in cancer","volume":"2","author":"M Holzlechner","year":"2019","journal-title":"Cancer Rep (Hoboken)."},{"key":"pone.0261491.ref016","doi-asserted-by":"crossref","first-page":"5959","DOI":"10.1038\/srep05959","article-title":"Significantly increased monounsaturated lipids relative to polyunsaturated lipids in six types of cancer microenvironment are observed by mass spectrometry imaging","volume":"4","author":"S Guo","year":"2014","journal-title":"Sci Rep"},{"key":"pone.0261491.ref017","doi-asserted-by":"crossref","first-page":"e90242","DOI":"10.1371\/journal.pone.0090242","article-title":"The expression profile of phosphatidylinositol in high spatial resolution imaging mass spectrometry as a potential biomarker for prostate cancer","volume":"9","author":"T Goto","year":"2014","journal-title":"PLoS One"},{"key":"pone.0261491.ref018","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1111\/cas.12229","article-title":"High-resolution imaging mass spectrometry reveals detailed spatial distribution of phosphatidylinositols in human breast cancer","volume":"104","author":"M Kawashima","year":"2013","journal-title":"Cancer Sci"},{"key":"pone.0261491.ref019","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1002\/pros.23088","article-title":"Decreased expression of lysophosphatidylcholine (16:0\/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer.","volume":"75","author":"T Goto","year":"2015","journal-title":"Prostate"},{"key":"pone.0261491.ref020","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.18632\/oncotarget.27649","article-title":"Lipid and protein tumor markers for head and neck squamous cell carcinoma identified by imaging mass spectrometry","volume":"11","author":"J Schmidt","year":"2020","journal-title":"Oncotarget"},{"key":"pone.0261491.ref021","doi-asserted-by":"crossref","first-page":"e61204","DOI":"10.1371\/journal.pone.0061204","article-title":"Human breast cancer tissues contain abundant phosphatidylcholine (36\u22361) with high stearoyl-CoA desaturase-1 expression.","volume":"8","author":"Y Ide","year":"2013","journal-title":"PLoS One"},{"key":"pone.0261491.ref022","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1002\/ijc.29517","article-title":"Non-small cell lung cancer is characterized by dramatic changes in phospholipid profiles","volume":"137","author":"E Marien","year":"2015","journal-title":"Int J Cancer"},{"key":"pone.0261491.ref023","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1002\/pmic.201300434","article-title":"MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma","volume":"14","author":"EE Jones","year":"2014","journal-title":"Proteomics"},{"key":"pone.0261491.ref024","doi-asserted-by":"crossref","first-page":"5387913","DOI":"10.1155\/2017\/5387913","article-title":"The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma","volume":"2017","author":"S Ishikawa","year":"2017","journal-title":"Biomed Res Int"},{"key":"pone.0261491.ref025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10735-018-9802-3","article-title":"Discrimination of normal oral mucosa from oral cancer by mass spectrometry imaging of proteins and lipids","volume":"50","author":"K Bednarczyk","year":"2019","journal-title":"J Mol Histol"},{"key":"pone.0261491.ref026","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1007\/s00216-013-7062-3","article-title":"Imaging mass spectrometry distinguished the cancer and stromal regions of oral squamous cell carcinoma by visualizing phosphatidylcholine (16:0\/16:1) and phosphatidylcholine (18:1\/20:4).","volume":"406","author":"Y Uchiyama","year":"2014","journal-title":"Anal Bioanal Chem"},{"key":"pone.0261491.ref027","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/modpathol.3800983","article-title":"Hierarchical cluster analysis of myoepithelial\/basal cell markers in adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma","volume":"21","author":"ML Prasad","year":"2008","journal-title":"Mod Pathol"},{"key":"pone.0261491.ref028","doi-asserted-by":"crossref","first-page":"55","DOI":"10.5858\/arpa.2014-0167-RA","article-title":"Review and updates of immunohistochemistry in selected salivary gland and head and neck tumors","volume":"139","author":"S Zhu","year":"2015","journal-title":"Arch Pathol Lab Med"},{"key":"pone.0261491.ref029","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1158\/1078-0432.CCR-03-0253","article-title":"HER2 expression in salivary gland carcinomas: dependence on histological subtype","volume":"10","author":"B Glisson","year":"2004","journal-title":"Clin Cancer Res"},{"key":"pone.0261491.ref030","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1093\/annonc\/mdx399","article-title":"Comprehensive genomic profiles of metastatic and relapsed salivary gland carcinomas are associated with tumor type and reveal new routes to targeted therapies.","volume":"28","author":"JS Ross","year":"2017","journal-title":"Ann Oncol"},{"key":"pone.0261491.ref031","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1039\/C4MB00586D","article-title":"Applications of mass spectrometry for cellular lipid analysis.","volume":"11","author":"C Wang","year":"2015","journal-title":"Mol Biosyst"},{"key":"pone.0261491.ref032","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.jasms.2010.03.028","article-title":"Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord","volume":"21","author":"M Girod","year":"2010","journal-title":"J Am Soc Mass Spectrom"},{"key":"pone.0261491.ref033","doi-asserted-by":"crossref","first-page":"e4491","DOI":"10.1002\/jms.4491","article-title":"Uncovering matrix effects on lipid analyses in MALDI imaging mass spectrometry experiments","volume":"55","author":"WJ Perry","year":"2020","journal-title":"J Mass Spectrom"},{"key":"pone.0261491.ref034","doi-asserted-by":"crossref","first-page":"3620","DOI":"10.1021\/acs.jproteome.0c00443","article-title":"Considerations for MALDI-Based Quantitative Mass Spectrometry Imaging Studies","volume":"19","author":"F Tobias","year":"2020","journal-title":"J Proteome Res"},{"key":"pone.0261491.ref035","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s12943-017-0646-3","article-title":"Emerging roles of lipid metabolism in cancer metastasis","volume":"16","author":"X Luo","year":"2017","journal-title":"Mol 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