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Mucosal subepithelial PDGFR\u03b1<jats:sup>+<\/jats:sup> cells (MuP\u03b1C) are important regulators in various intestinal diseases including fibrosis and inflammation. However, the transcriptome of MuP\u03b1C has not yet been elucidated. Using Pdgfra-eGFP mice and flow cytometry, we isolated colonic MuP\u03b1C and obtained their transcriptome data. In analyzing the transcriptome, we identified three novel, and selectively expressed, markers (<jats:italic>Adamdec1<\/jats:italic>, <jats:italic>Fin1<\/jats:italic>, and <jats:italic>Col6a4<\/jats:italic>) found in MuP\u03b1C. In addition, we identified a unique set of MuP\u03b1C-enriched genetic signatures including groups of growth factors, transcription factors, gap junction proteins, extracellular proteins, receptors, cytokines, protein kinases, phosphatases, and peptidases. These selective groups of genetic signatures are linked to the unique cellular identity and function of MuP\u03b1C. Furthermore, we have added this MuP\u03b1C transcriptome data to our Smooth Muscle Genome Browser that contains the transcriptome data of jejunal and colonic smooth muscle cells (SMC), interstitial cells of Cajal (ICC), and smooth muscle resident PDGFR\u03b1<jats:sup>+<\/jats:sup> cells: (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/med.unr.edu\/physio\/transcriptome\" xlink:type=\"simple\">https:\/\/med.unr.edu\/physio\/transcriptome<\/jats:ext-link>). This online resource provides a comprehensive reference of all currently known genetic transcripts expressed in primary MuP\u03b1C in the colon along with smooth muscle resident PDGFR\u03b1 cells, SMC, and ICC in the murine colon and jejunum.<\/jats:p>","DOI":"10.1371\/journal.pone.0261743","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T17:34:46Z","timestamp":1652463286000},"page":"e0261743","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transcriptome profiling of subepithelial PDGFR\u03b1 cells in colonic mucosa reveals several cell-selective markers"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6063-5559","authenticated-orcid":true,"given":"Se 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