{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:32:53Z","timestamp":1791289973182,"version":"4.3.0"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T00:00:00Z","timestamp":1640736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Antimicrobial Resistance Cross Council Initiative supported by the seven research councils in partnership with the Department of Health and Department for Environment Food & Rural Affairs","award":["NE\/N019555\/1"],"award-info":[{"award-number":["NE\/N019555\/1"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Fluro(quinolones) is an important class of antibiotic used widely in both human and veterinary medicine. Resistance to fluro(quinolones) can be acquired by either chromosomal point mutations or plasmid-mediated quinolone resistance (PMQR). There is a lack of studies on the prevalence of PMQR in organisms from environmental sources in Bangladesh. In this study, we investigated the occurrence of PMQR genes in <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> from various water sources and analysed associations between multi-drug resistance (MDR) and resistance to extended spectrum \u03b2-lactam antibiotics. We analysed 300 <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates from wastewaters of urban live-bird markets (n = 74) and rural households (n = 80), rural ponds (n = 71) and river water samples (n = 75) during 2017\u20132018. We isolated <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> by filtering 100 ml of water samples through a 0.2\u03bcm cellulose membrane and incubating on mTEC agar media followed by identification of isolated colonies using biochemical tests. We selected one isolate per sample for detection of PMQR genes by multiplex PCR and tested for antibiotic susceptibility by disc diffusion. Clonal relatedness of PMQR-positive isolates was evaluated by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). About 66% (n = 199) of <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates harbored PMQR-genes, predominantly <jats:italic>qnrS<\/jats:italic> (82%, n = 164) followed by <jats:italic>aac(6\u2019)-lb-cr<\/jats:italic> (9%, n = 17), <jats:italic>oqxAB<\/jats:italic> (7%, n = 13), <jats:italic>qnrB<\/jats:italic> (6%, n = 11) and <jats:italic>qepA<\/jats:italic> (4%, n = 8). Around 68% (n = 135) of PMQR-positive isolates were MDR and 92% (n = 183) were extended spectrum \u03b2-lactamase (ESBL)-producing of which the proportion of positive samples was 87% (n = 159) for <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-1\u2019<\/jats:sub> 34% (n = 62) for <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>, 9% (n = 16) for <jats:italic>bla<\/jats:italic><jats:sub>OXA-1,<\/jats:sub> <jats:italic>bla<\/jats:italic><jats:sub>OXA-47<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>CMY-2<\/jats:sub>, and 2% (n = 4) for <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>. Further, 16% (n = 32) of PMQR-positive isolates were resistant to carbapenems of which 20 isolates carried <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub>. Class 1 integron (<jats:italic>int<\/jats:italic>1) was found in 36% (n = 72) of PMQR-positive <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates. PMQR genes were significantly associated with ESBL phenotypes (<jats:italic>p<\/jats:italic>\u22640.001). The presence of several PMQR genes were positively associated with ESBL and carbapenemase encoding genes such as <jats:italic>qnrS<\/jats:italic> with <jats:italic>bla<\/jats:italic><jats:sub>CTXM-1<\/jats:sub> (<jats:italic>p<\/jats:italic>&lt;0.001), <jats:italic>qnrB<\/jats:italic> with <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> (<jats:italic>p<\/jats:italic>&lt;0.001) and <jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub> (<jats:italic>p<\/jats:italic> = 0.005), <jats:italic>oqxAB<\/jats:italic> and <jats:italic>aac(6\u2019)-lb-cr<\/jats:italic> with <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub> (<jats:italic>p<\/jats:italic>&lt;0.001), <jats:italic>qnrB<\/jats:italic> with <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> (<jats:italic>p<\/jats:italic>&lt;0.001), <jats:italic>aac(6\u2019)-lb-cr<\/jats:italic> with <jats:italic>bla<\/jats:italic><jats:sub>OXA-47<\/jats:sub> (<jats:italic>p<\/jats:italic>&lt;0.001) and <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> (<jats:italic>p<\/jats:italic> = 0.002). Further, <jats:italic>int<\/jats:italic>1 was found to correlate with <jats:italic>qnrB<\/jats:italic> (<jats:italic>p<\/jats:italic>&lt;0.001) and <jats:italic>qepA<\/jats:italic> (<jats:italic>p<\/jats:italic> = 0.011). ERIC-PCR profiles allowed identification of 84 of 199 isolates with 85% matching profiles which were further grouped into 33 clusters. Only 5 clusters had isolates (n = 11) with identical ERIC-PCR profiles suggesting that PMQR-positive <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates are genetically heterogeneous. Overall, PMQR-positive MDR <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> were widely distributed in aquatic environments of Bangladesh indicating poor wastewater treatment and highlighting the risk of transmission to humans and animals.<\/jats:p>","DOI":"10.1371\/journal.pone.0261970","type":"journal-article","created":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T18:34:04Z","timestamp":1640802844000},"page":"e0261970","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["High prevalence of plasmid-mediated quinolone resistance (PMQR) among E. coli from aquatic environments in Bangladesh"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7181-6432","authenticated-orcid":true,"given":"Mohammed Badrul","family":"Amin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumita Rani","family":"Saha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Rayhanul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7527-1310","authenticated-orcid":true,"given":"S. M. Arefeen","family":"Haider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammed Iqbal","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S. M. Homaun Kabir","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily K.","family":"Rousham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Aminul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,12,29]]},"reference":[{"key":"pone.0261970.ref001","author":"J. 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