{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:27:04Z","timestamp":1772213224923,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T00:00:00Z","timestamp":1640908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Toyobo"},{"name":"Toyobo"},{"name":"Toyobo"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      GENECUBE\n                      <jats:sup>\u00ae<\/jats:sup>\n                      is a rapid molecular identification system, and previous studies demonstrated that GENECUBE\n                      <jats:sup>\u00ae<\/jats:sup>\n                      HQ SARS-CoV-2 showed excellent analytical performance for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with nasopharyngeal samples. However, other respiratory samples have not been evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This prospective comparison between GENECUBE\n                      <jats:sup>\u00ae<\/jats:sup>\n                      HQ SARS-CoV-2 and reference real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for the detection of SARS-CoV-2 using anterior nasal samples and saliva samples. Additionally, we evaluated a new rapid examination protocol using GENECUBE\n                      <jats:sup>\u00ae<\/jats:sup>\n                      HQ SARS-CoV-2 for the detection of SARS-CoV-2 with saliva samples. For the rapid protocol, in the preparation of saliva samples, purification and extraction processes were adjusted, and the total process time was shortened to approximately 35 minutes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>For 359 anterior nasal samples, the total-, positive-, and negative concordance of the two assays was 99.7% (358\/359), 98.1% (51\/52), and 100% (307\/307), respectively. For saliva samples, the total-, positive-, and negative concordance of the two assays was 99.6% (239\/240), 100% (56\/56), and 99.5% (183\/184), respectively. With the new protocol, total-, positive-, and negative concordance of the two assays was 98.8% (237\/240), 100% (56\/56), and 98.4% (181\/184), respectively. In all discordance cases, SARS-CoV-2 was detected by additional molecular examinations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      GENECUBE\n                      <jats:sup>\u00ae<\/jats:sup>\n                      HQ SARS-CoV-2 provided high analytical performance for the detection of SARS-CoV-2 in anterior nasal samples and saliva samples.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0262159","type":"journal-article","created":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T13:42:17Z","timestamp":1640958137000},"page":"e0262159","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new 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