{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:15:54Z","timestamp":1784081754444,"version":"3.55.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Published cerebrovascular injection techniques have mostly used decapitated, fresh cadavers or heads embalmed with 10% formaldehyde. There have been no reports using vascular-injected cadavers for head and neck surgical training models or using vascular injections in saturated salt method-embalmed cadavers. Thus, we performed vascular labeling of five saturated salt method-embalmed cadavers without decapitation. Latex mixed with red ink was injected into the common carotid artery via a 3D-printed vascular adapter. The injection force was provided by a peristaltic pump. Thyroidectomy, submandibular gland excision, neck dissection, parotidectomy, and mandibulotomy were performed on both sides of each cadaver (n = 10). The consistency of the cadavers was softer than fresh ones. Subcutaneous tissues were well preserved, and muscles were moist and elastic. Five physicians graded the resemblance of the heads and necks of the latex-injected, saturated salt method-embalmed, non-decapitated of five cadavers compared to living humans using a Likert scale from 0 (no resemblance) to 5 (maximum resemblance). Fifty-two percent of the head and neck region resemblance scale ratings were four or five. Although the cadavers were practical for head and neck surgical simulations, the brain parenchyma was only partially preserved and unsuitable for use. The most distal arterial branches reached by the injected latex were measured. The external caliber of the smallest vessels reached were lacrimal arteries (mean caliber \u00b1 SD, 0.04 \u00b1 0.04 mm; 95% CI [0, 0.09]). There were no significant differences in the mean caliber of the smallest vessels reached between the left- and right-sided arterial branches (all p &lt; 0.05).<\/jats:p>","DOI":"10.1371\/journal.pone.0262415","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T18:33:12Z","timestamp":1642703592000},"page":"e0262415","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Latex-injected, non-decapitated, saturated salt method-embalmed cadaver technique development and application as a head and neck surgery training 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Caraballo","year":"2017","journal-title":"Implant Dent."},{"issue":"5","key":"pone.0262415.ref014","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1097\/00006123-199911000-00058","article-title":"Colored silicone injection for use in neurosurgical dissections: anatomic technical note.","volume":"45","author":"A Sanan","year":"1999","journal-title":"Neurosurgery."},{"issue":"2","key":"pone.0262415.ref015","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jsurg.2016.09.007","article-title":"Current Status of Simulation in Otolaryngology: A Systematic Review.","volume":"74","author":"O Musbahi","year":"2017","journal-title":"J Surg Educ"},{"issue":"1","key":"pone.0262415.ref016","first-page":"5","article-title":"Current State of Surgical Simulation Training in Otolaryngology: Systematic Review of Simulation Training Models.","volume":"3","author":"A Mohamedkazim","year":"2019","journal-title":"Archives of Otorhinolaryngology-Head & Neck Surgery"},{"issue":"Pt 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Krejza","year":"2006","journal-title":"Stroke"},{"key":"pone.0262415.ref023","doi-asserted-by":"crossref","first-page":"27","DOI":"10.2147\/RMHP.S10383","article-title":"Anatomic considerations for central venous cannulation.","volume":"4","author":"MP Bannon","year":"2011","journal-title":"Risk Manag Healthc Policy"},{"key":"pone.0262415.ref024","first-page":"1562","author":"S. Standring","year":"2016","edition":"41"},{"issue":"5","key":"pone.0262415.ref025","doi-asserted-by":"crossref","first-page":"382","DOI":"10.61872\/sdj-2018-05-405","article-title":"The Face\u2014A Vascular Perspective. A literature review","volume":"128","author":"T von Arx","year":"2018","journal-title":"Swiss Dent J"},{"key":"pone.0262415.ref026","unstructured":"Department TM. Annual weather summary 2020 [cited 2021 Dec 14]. Available from: https:\/\/www.tmd.go.th\/climate\/climate.php?FileID=5."},{"issue":"1","key":"pone.0262415.ref027","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12565-015-0299-5","article-title":"History and future of human cadaver preservation for surgical training: from formalin to saturated salt solution method.","volume":"91","author":"S Hayashi","year":"2016","journal-title":"Anat Sci Int"},{"issue":"10","key":"pone.0262415.ref028","doi-asserted-by":"crossref","first-page":"e543","DOI":"10.1097\/GOX.0000000000000523","article-title":"Experience of Raising Flaps Using Cadavers Embalmed by Saturated Salt Solution Method.","volume":"3","author":"T Shirai","year":"2015","journal-title":"Plast Reconstr Surg Glob Open"},{"issue":"1","key":"pone.0262415.ref029","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1097\/MLG.0b013e318155a492","article-title":"Endoscopic anatomy of the pterygopalatine fossa and the transpterygoid approach: development of a surgical instruction model","volume":"118","author":"FS Fortes","year":"2008","journal-title":"Laryngoscope"},{"issue":"8","key":"pone.0262415.ref030","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1002\/hed.23418","article-title":"Surgical anatomy of the supraglottic larynx using the da Vinci robot.","volume":"36","author":"N Goyal","year":"2014","journal-title":"Head Neck"},{"issue":"4","key":"pone.0262415.ref031","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1111\/joa.12504","article-title":"A comparative study of vascular injection fluids in fresh-frozen and embalmed human cadaver forearms.","volume":"229","author":"DE Doomernik","year":"2016","journal-title":"J Anat"},{"key":"pone.0262415.ref032","unstructured":"Microfil Silicone Rubber Injection Compounds [cited 2021 Dec 3]. Available from: https:\/\/www.laddresearch.com\/chemicals\/microfil-silicone-rubber-injection-compounds."},{"issue":"6","key":"pone.0262415.ref033","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1097\/PRS.0b013e3181a3f3ae","article-title":"An anatomical study of external carotid artery vascular territories in face and midface flaps for transplantation.","volume":"123","author":"ND Banks","year":"2009","journal-title":"Plast Reconstr Surg"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0262415","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T17:33:25Z","timestamp":1726508005000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0262415"}},"subtitle":[],"editor":[{"given":"Eng","family":"Ooi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,1,20]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0262415","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,20]]}}}