{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:37:32Z","timestamp":1792802252357,"version":"4.5.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T00:00:00Z","timestamp":1641513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"St John of God Subiaco Hospital","id":[{"id":"https:\/\/ror.org\/00hvh1x59","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mucosal infiltration by certain bacterial species may contribute to the development and progression of colorectal cancer (CRC). There is considerable variation in reported detection rates in human CRC samples and the extent to which bacterial infiltration varies across regions of the primary tumour is unknown. This study aimed to determine if there is an optimal site for bacterial detection within CRC tumours.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Presence of target bacterial species was assessed by quantitative real-time PCR (qPCR) in 42 human CRC tumours. Abundance in primary tumour regions, normal epithelium and at metastatic sites was investigated in an expanded cohort of 51 patients. Species presence\/absence was confirmed by diversity profiling in five patients. Correlation with total bacterial load and clinicopathological features was assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                      and\n                      <jats:italic>Bacteroides fragilis<\/jats:italic>\n                      were detected in tumours from 43% and 24% of patients, respectively (17% positive for both species). The optimal detection site was the tumour luminal surface (TLS). Patients testing positive at the TLS frequently tested negative at other sites, including central tumour and invasive margin.\n                      <jats:italic>F<\/jats:italic>\n                      .\n                      <jats:italic>nucleatum<\/jats:italic>\n                      was detected at a higher frequency in tumour versus normal epithelium (p &lt; 0.01) and was associated with more advanced disease (p = 0.01). Detection of both species correlated with total bacterial load. However, corroboration of qPCR results via diversity profiling suggests detection of these species may indicate a specific microbial signature.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study supports a role for\n                      <jats:italic>F<\/jats:italic>\n                      .\n                      <jats:italic>nucleatum<\/jats:italic>\n                      in CRC development. Presence of\n                      <jats:italic>F<\/jats:italic>\n                      .\n                      <jats:italic>nucleatum<\/jats:italic>\n                      and\n                      <jats:italic>B<\/jats:italic>\n                      .\n                      <jats:italic>fragilis<\/jats:italic>\n                      varies across primary tumour regions, with the TLS representing the optimal site for bacterial detection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0262416","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T13:30:39Z","timestamp":1641562239000},"page":"e0262416","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Fusobacterium nucleatum and Bacteroides fragilis detection in colorectal tumours: Optimal target site and correlation with total bacterial 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