{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:27:22Z","timestamp":1792074442361,"version":"4.4.0"},"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Steno Diabetes Centre North Denmark"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>The incidence of women developing gestational diabetes mellitus (GDM) is increasing, which is associated with an increased risk of type 2 diabetes mellitus (T2DM) for both mother and child. Gut microbiota dysbiosis may contribute to the pathogenesis of both GDM and the accompanying risk of T2DM. Thus, a better understanding of the microbial communities associated with GDM could offer a potential target for intervention and treatment in the future. Therefore, we performed a systematic review to investigate if the GDM women have a distinct gut microbiota composition compared to non-GDM women.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>We identified 21 studies in a systematic literature search of Embase and PubMed up to February 24, 2021. Data on demographics, methodology and identified microbial metrics were extracted. The quality of each study was assessed according to the Newcastle-Ottawa Scale.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Sixteen of the studies did find a GDM-associated gut microbiota, although no consistency could be seen. Only <jats:italic>Collinsella<\/jats:italic> and <jats:italic>Blautia<\/jats:italic> showed a tendency to be increased in GDM women, whereas the remaining genera were significantly different in opposing directions.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Although most of the studies found an association between GDM and gut microbiota dysbiosis, no overall GDM-specific gut microbiota could be identified. All studies in the second trimester found a difference between GDM and non-GDM women, indicating that dysbiosis is present at the time of diagnosis. Nevertheless, it is still unclear when the dysbiosis develops, as no consensus could be seen between the studies investigating the gut microbiota in the first trimester of pregnancy. However, studies varied widely concerning methodology and study design, which might explain the highly heterogeneous gut microbiota compositions between studies. Therefore, future studies need to include multiple time points and consider possible confounding factors such as ethnicity, pre-pregnancy body mass index, and GDM treatment.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0262618","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T18:59:05Z","timestamp":1642100345000},"page":"e0262618","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Characteristics of the gut microbiome in women with gestational diabetes mellitus: A systematic review"],"prefix":"10.1371","volume":"17","author":[{"given":"Louise S\u00f8ndergaard","family":"Rold","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caspar","family":"Bundgaard-Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Niemann Holm-Jacobsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Glud Ovesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Leutscher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1094-6575","authenticated-orcid":true,"given":"S\u00f8ren","family":"Hagstr\u00f8m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4201-1168","authenticated-orcid":true,"given":"Suzette","family":"S\u00f8rensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,1,13]]},"reference":[{"key":"pone.0262618.ref001","doi-asserted-by":"crossref","first-page":"4","DOI":"10.4161\/gmic.19320","article-title":"The role of gut microbiota in immune homeostasis and autoimmunity","volume":"3","author":"H-J Wu","year":"2012","journal-title":"Gut Microbes"},{"key":"pone.0262618.ref002","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1038\/nature05414","article-title":"An obesity-associated gut microbiome with increased capacity for energy harvest","volume":"444","author":"PJ Turnbaugh","year":"2006","journal-title":"Nature"},{"key":"pone.0262618.ref003","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.celrep.2019.03.075","article-title":"Progesterone Increases Bifidobacterium Relative Abundance during Late Pregnancy","volume":"27","author":"M Nuriel-Ohayon","year":"2019","journal-title":"Cell Rep"},{"key":"pone.0262618.ref004","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/femsre\/fuu010","article-title":"Microbial endocrinology: the interplay between the microbiota and the endocrine system","volume":"39","author":"H Neuman","year":"2015","journal-title":"FEMS Microbiol 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metabolic syndrome in youth with obesity: a pilot study","author":"F Del Chierico","year":"2021","journal-title":"Acta Diabetol"},{"key":"pone.0262618.ref009","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature07540","article-title":"A core gut microbiome in obese and lean twins","volume":"457","author":"PJ Turnbaugh","year":"2009","journal-title":"Nature"},{"key":"pone.0262618.ref010","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.chom.2008.02.015","article-title":"Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome","volume":"3","author":"PJ Turnbaugh","year":"2008","journal-title":"Cell Host Microbe"},{"key":"pone.0262618.ref011","doi-asserted-by":"crossref","first-page":"1241214","DOI":"10.1126\/science.1241214","article-title":"Gut microbiota from twins discordant for obesity modulate metabolism in mice","volume":"341","author":"VK Ridaura","year":"2013","journal-title":"Science"},{"key":"pone.0262618.ref012","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.cell.2012.07.008","article-title":"Host remodeling of the gut microbiome and metabolic changes during pregnancy","volume":"150","author":"O Koren","year":"2012","journal-title":"Cell"},{"key":"pone.0262618.ref013","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41579-020-0433-9","article-title":"Gut microbiota in human metabolic health and disease","volume":"19","author":"Y Fan","year":"2021","journal-title":"Nat Rev Microbiol"},{"key":"pone.0262618.ref014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/microorganisms9040697","article-title":"The Role of Enterobacteriaceae in Gut Microbiota Dysbiosis in Inflammatory Bowel Diseases.","volume":"9","author":"V Baldelli","year":"2021","journal-title":"Microorganisms"},{"key":"pone.0262618.ref015","doi-asserted-by":"crossref","first-page":"e35240","DOI":"10.1371\/journal.pone.0035240","article-title":"Butyrate and propionate protect against diet-induced obesity and regulate gut hormones via free fatty acid receptor 3-independent mechanisms","volume":"7","author":"H V. Lin","year":"2012","journal-title":"PLoS One"},{"key":"pone.0262618.ref016","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1073\/pnas.1322269111","article-title":"The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition","volume":"111","author":"P V. Chang","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0262618.ref017","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1053\/j.gastro.2012.06.031","article-title":"Transfer of intestinal microbiota from lean donors increases insulin sensitivity in individuals with metabolic syndrome","volume":"143","author":"A Vrieze","year":"2012","journal-title":"Gastroenterology"},{"key":"pone.0262618.ref018","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/S0002-9378(99)70662-9","article-title":"Longitudinal changes in glucose metabolism during pregnancy in obese women with normal glucose tolerance and gestational diabetes mellitus","volume":"180","author":"PM Catalano","year":"1999","journal-title":"Am J Obstet Gynecol"},{"issue":"Suppl 2","key":"pone.0262618.ref019","doi-asserted-by":"crossref","first-page":"S112","DOI":"10.2337\/dc07-s202","article-title":"Cellular mechanisms for insulin resistance in normal pregnancy and gestational diabetes","volume":"30","author":"LA Barbour","year":"2007","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref020","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/S0002-9378(12)80011-1","article-title":"Longitudinal changes in basal hepatic glucose production and suppression during insulin infusion in normal pregnant women","volume":"167","author":"PM Catalano","year":"1992","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0262618.ref021","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.2337\/dc15-2672","article-title":"Heterogeneous Contribution of Insulin Sensitivity and Secretion Defects to Gestational Diabetes Mellitus","volume":"39","author":"CE Powe","year":"2016","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref022","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1186\/s12967-018-1666-5","article-title":"Heterogeneity of insulin resistance and beta cell dysfunction in gestational diabetes mellitus: a prospective cohort study of perinatal outcomes","volume":"16","author":"Y Liu","year":"2018","journal-title":"J Transl Med"},{"key":"pone.0262618.ref023","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1007\/s00125-020-05323-0","article-title":"Do variations in insulin sensitivity and insulin secretion in pregnancy predict differences in obstetric and neonatal outcomes?","volume":"64","author":"LR Madsen","year":"2021","journal-title":"Diabetologia"},{"key":"pone.0262618.ref024","doi-asserted-by":"crossref","first-page":"780","DOI":"10.2337\/dc11-1790","article-title":"The hyperglycemia and adverse pregnancy outcome study: associations of GDM and obesity with pregnancy outcomes","volume":"35","author":"PM Catalano","year":"2012","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref025","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1056\/NEJMoa042973","article-title":"Effect of treatment of gestational diabetes mellitus on pregnancy outcomes","volume":"352","author":"CA Crowther","year":"2005","journal-title":"N Engl J Med"},{"key":"pone.0262618.ref026","first-page":"11","article-title":"The effect of different forms of dysglycemia during pregnancy on maternal and fetal outcomes in treated women and comparison with large cohort studies","volume":"89","author":"A Soliman","year":"2018","journal-title":"Acta Biomed"},{"key":"pone.0262618.ref027","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1056\/NEJMoa0707943","article-title":"Hyperglycemia and adverse pregnancy outcomes","volume":"358","author":"HAPO Study Cooperative Research Group","year":"2008","journal-title":"N Engl J Med"},{"key":"pone.0262618.ref028","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.2337\/db15-1720","article-title":"A Predictive Metabolic Signature for the Transition From Gestational Diabetes Mellitus to Type 2 Diabetes","volume":"65","author":"A Allalou","year":"2016","journal-title":"Diabetes"},{"key":"pone.0262618.ref029","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.2337\/diacare.25.10.1862","article-title":"Gestational diabetes and the incidence of type 2 diabetes: a systematic review","volume":"25","author":"C Kim","year":"2002","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref030","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.diabres.2007.03.023","article-title":"Early manifestation of cardiovascular disease risk factors in offspring of mothers with previous history of gestational diabetes mellitus","volume":"78","author":"H Lee","year":"2007","journal-title":"Diabetes Res Clin Pract"},{"key":"pone.0262618.ref031","doi-asserted-by":"crossref","first-page":"340","DOI":"10.2337\/dc07-1596","article-title":"High prevalence of type 2 diabetes and pre-diabetes in adult offspring of women with gestational diabetes mellitus or type 1 diabetes: the role of intrauterine hyperglycemia","volume":"31","author":"TD Clausen","year":"2008","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref032","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.2337\/diacare.27.5.1194","article-title":"Increasing incidence of diabetes after gestational diabetes: a long-term follow-up in a Danish population","volume":"27","author":"J Lauenborg","year":"2004","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref033","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1007\/s00125-016-3985-5","article-title":"Gestational diabetes mellitus and long-term consequences for mother and offspring: a view from Denmark","volume":"59","author":"P Damm","year":"2016","journal-title":"Diabetologia"},{"key":"pone.0262618.ref034","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1016\/S0140-6736(09)60731-5","article-title":"Type 2 diabetes mellitus after gestational diabetes: a systematic review and meta-analysis","volume":"373","author":"L Bellamy","year":"2009","journal-title":"Lancet (London, England)."},{"key":"pone.0262618.ref035","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1001\/jama.2018.11628","article-title":"Association of Gestational Diabetes With Maternal Disorders of Glucose Metabolism and Childhood Adiposity","volume":"320","author":"WL Lowe","year":"2018","journal-title":"JAMA"},{"key":"pone.0262618.ref036","doi-asserted-by":"crossref","first-page":"m1361","DOI":"10.1136\/bmj.m1361","article-title":"Progression to type 2 diabetes in women with a known history of gestational diabetes: systematic review and meta-analysis","volume":"369","author":"E Vounzoulaki","year":"2020","journal-title":"BMJ"},{"key":"pone.0262618.ref037","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.2337\/dc06-2559a","article-title":"Maternal obesity and risk of gestational diabetes mellitus","volume":"30","author":"SY Chu","year":"2007","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref038","doi-asserted-by":"crossref","first-page":"676","DOI":"10.2337\/dc09-1848","article-title":"International association of diabetes and pregnancy study groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy","volume":"33","author":"International Association of Diabetes and Pregnancy Study Groups Consensus Panel","year":"2010","journal-title":"Diabetes Care"},{"key":"pone.0262618.ref039","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41572-019-0098-8","article-title":"Gestational diabetes mellitus","volume":"5","author":"HD McIntyre","year":"2019","journal-title":"Nat Rev Dis Prim"},{"key":"pone.0262618.ref040","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1017\/S2040174420000768","article-title":"Perturbations of gut microbiota in gestational diabetes mellitus patients induce hyperglycemia in germ-free mice","volume":"11","author":"Y Liu","year":"2020","journal-title":"J Dev Orig Health Dis"},{"key":"pone.0262618.ref041","doi-asserted-by":"crossref","first-page":"e1000097","DOI":"10.1371\/journal.pmed.1000097","article-title":"Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement.","volume":"6","author":"PRISMA Group","year":"2009","journal-title":"PLoS Med"},{"key":"pone.0262618.ref042","article-title":"The Newcastle-Ottawa Scale (NOS) for Assessing the Quality of Nonrandomized Studies in Meta- Analysis Bias and Confounding Newcastle-Ottowa Scale","author":"G Wells","year":"2012","journal-title":"Ottawa Hosp Res Inst"},{"key":"pone.0262618.ref043","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1080\/19490976.2020.1748258","article-title":"Gut microbiota profiles of autism spectrum disorder and attention deficit\/hyperactivity disorder: A systematic literature review","volume":"11","author":"C Bundgaard-Nielsen","year":"2020","journal-title":"Gut 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Microbiol"},{"key":"pone.0262618.ref047","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1007\/s00592-017-1056-0","article-title":"Gut microbiota aberrations precede diagnosis of gestational diabetes mellitus","volume":"54","author":"K Mokkala","year":"2017","journal-title":"Acta Diabetol"},{"key":"pone.0262618.ref048","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.2337\/db16-0278","article-title":"Connections Between the Gut Microbiome and Metabolic Hormones in Early Pregnancy in Overweight and Obese Women","volume":"65","author":"LF Gomez-Arango","year":"2016","journal-title":"Diabetes"},{"key":"pone.0262618.ref049","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mSystems.00109-20","article-title":"Gestational Diabetes Mellitus Is Associated with Reduced Dynamics of Gut Microbiota during the First Half of Pregnancy","volume":"5","author":"W Zheng","year":"2020","journal-title":"mSystems"},{"key":"pone.0262618.ref050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/19490976.2020.1840765","article-title":"Altered gut bacterial and metabolic signatures and their interaction in gestational diabetes mellitus","volume":"12","author":"X Wang","year":"2020","journal-title":"Gut Microbes"},{"key":"pone.0262618.ref051","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1111\/jdi.13373","article-title":"Relationships between gut microbiota, plasma glucose and gestational diabetes mellitus.","volume":"12","author":"T Chen","year":"2021","journal-title":"J Diabetes Investig"},{"key":"pone.0262618.ref052","first-page":"1","article-title":"Connections between the human gut microbiome and gestational diabetes mellitus","volume":"6","author":"Y-S Kuang","year":"2017","journal-title":"Gigascience"},{"key":"pone.0262618.ref053","doi-asserted-by":"crossref","first-page":"E247","DOI":"10.1152\/ajpendo.00266.2019","article-title":"Differential intestinal and oral microbiota features associated with gestational diabetes and maternal inflammation","volume":"319","author":"Y Xu","year":"2020","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"pone.0262618.ref054","first-page":"8892849","article-title":"Correlation Analysis between GDM and Gut Microbial Composition","volume":"2021","author":"G Li","year":"2021","journal-title":"Late Pregnancy. 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A case-control study","volume":"12","author":"RA Ngala","year":"2017","journal-title":"PLoS One"},{"key":"pone.0262618.ref070","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.2337\/dc17-2393","article-title":"Gestational Diabetes Mellitus: Does One Size Fit All? 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