{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T18:07:23Z","timestamp":1788631643664,"version":"build-2803163510"},"reference-count":24,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"LIM 52, Laborat\u00f3rios de Investiga\u00e7\u00e3o M\u00e9dica - Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo"},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["401626\/2013-0"],"award-info":[{"award-number":["401626\/2013-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"IBEST","award":["P30GM103324"],"award-info":[{"award-number":["P30GM103324"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Torquetenovirus (TTV) is a nonpathogenic endogenous virus whose abundance varies with the extent of immune system activation. We determined if the TTV titer in the vagina of pregnant women was associated with vaginal microbiota composition and levels of compounds in vaginal secretions. Vaginal TTV and microbiota composition in 494 second trimester pregnant women were identified by gene amplification and analysis. Vaginal matrix metalloproteinases (MMPs), tissue inhibitors of MMP (TIMP) and lactic acid isomers were measured by ELISA. Dominance was defined as the relative abundance of a specific bacterium or species at &gt;50% of the total number of bacteria identified. Clinical data were obtained by chart review. The median log<jats:sub>10<\/jats:sub>TTV titer was lowest when<jats:italic>Lactobacillus<\/jats:italic>species other than<jats:italic>L<\/jats:italic>.<jats:italic>iners<\/jats:italic>were dominant (&lt;1.0) as compared to when<jats:italic>L<\/jats:italic>.<jats:italic>iners<\/jats:italic>(4.1, p = 0.0001), bacteria other than lactobacilli (4.5, p = 0.0016) or no bacterium (4.7, p = 0.0009) dominated. The TTV titer was inversely proportional to<jats:italic>L<\/jats:italic>.<jats:italic>crispatus<\/jats:italic>abundance (p&lt;0.0001) and directly proportional to levels of<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>(p = 0.0008) and<jats:italic>L<\/jats:italic>.<jats:italic>iners<\/jats:italic>(p = 0.0010). The TTV titer was proportional to TIMP-1, TIMP-2, MMP-8 and MMP-9 abundance (p\u22640.0002) and inversely proportional to the level of D-lactic acid (p = 0.0024). We conclude that the association between variations in the TTV titer and the relative abundance of specific bacterial species and vaginal compounds indicates that local changes in immune status likely influence vaginal fluid composition.<\/jats:p>","DOI":"10.1371\/journal.pone.0262672","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T18:28:31Z","timestamp":1642703311000},"page":"e0262672","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["The vaginal Torquetenovirus titer varies with vaginal microbiota composition in pregnant women"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5659-1052","authenticated-orcid":true,"given":"Tania Regina","family":"Tozetto-Mendoza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C.","family":"Mendes-Correa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio F.","family":"Moron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry J.","family":"Forney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iara M.","family":"Linhares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7071-8243","authenticated-orcid":true,"given":"Almir","family":"Ribeiro da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7084-3095","authenticated-orcid":true,"given":"Layla","family":"Honorato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven S.","family":"Witkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,1,20]]},"reference":[{"key":"pone.0262672.ref001","first-page":"21","article-title":"Classification of TTV and related viruses (anelloviruses)","volume":"331","author":"P. 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