{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:48:27Z","timestamp":1790178507570,"version":"4.1.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>This study was designed to explore the therapeutics and the mechanisms of a patented and marked gastric acid and intestine juice-resistant probiotics <jats:italic>Bifidobacterium lactis<\/jats:italic> BL-99 (<jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99) on the intestinal inflammation and functions in the zebrafish models. After feeding for 6 hours, <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99 was fully retained in the larval zebrafish intestinal tract and stayed for over 24 hours. <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99 promoted the intestinal motility and effectively alleviated aluminum sulfate-induced larval zebrafish constipation (<jats:italic>p<\/jats:italic> &lt; 0.01). Irregular high glucose diet induced adult zebrafish intestinal functional and metabolic disorders. After fed with <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99, <jats:italic>IL-1\u03b2<\/jats:italic> gene expression was significantly down-regulated, and <jats:italic>IL-10<\/jats:italic> and <jats:italic>IL-12<\/jats:italic> gene levels were markedly up-regulated in this model (<jats:italic>p<\/jats:italic> &lt; 0.05). The intestinal lipase activity was elevated in the adult zebrafish intestinal functional disorder model after <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99 treatment (<jats:italic>p<\/jats:italic> &lt; 0.05), but tryptase content had no statistical changes (<jats:italic>p<\/jats:italic> &gt; 0.05). <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99 improved the histopathology of the adult zebrafish intestinal inflammation, increased the goblet cell numbers, and up-and-down metabolites were markedly recovered after treatment of <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99 (<jats:italic>p<\/jats:italic> &lt; 0.05). These results suggest that <jats:italic>B<\/jats:italic>. <jats:italic>lactis<\/jats:italic> BL-99 could relieve intestinal inflammation and promote intestinal functions, at least in part, through modulating intestinal and microbial metabolism to maintain intestinal health.<\/jats:p>","DOI":"10.1371\/journal.pone.0262942","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T18:41:04Z","timestamp":1645036864000},"page":"e0262942","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models"],"prefix":"10.1371","volume":"17","author":[{"given":"Meng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinfeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhu","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4223-170X","authenticated-orcid":true,"given":"Weilian","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,2,16]]},"reference":[{"key":"pone.0262942.ref001","first-page":"1","article-title":"Trends in the use of complementary health approaches among adults: United States, 2002\u20132012","volume":"79","author":"TC Clarke","year":"2015","journal-title":"Natl Health Stat Report"},{"key":"pone.0262942.ref002","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1038\/s41591-019-0439-x","article-title":"The pros, cons, and many unknowns of probiotics","volume":"25","author":"J Suez","year":"2019","journal-title":"Nat Med"},{"key":"pone.0262942.ref003","first-page":"237","article-title":"Probiotics: achieving a better regulatory fit.","volume":"69","author":"DE Hofmann","year":"2014","journal-title":"Food Drug Law J"},{"key":"pone.0262942.ref004","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fsi.2021.07.007","article-title":"The functionality of probiotics in aquaculture: An overview","volume":"117","author":"MT El-Saadony","year":"2021","journal-title":"Fish Shellfish Immunol"},{"issue":"2","key":"pone.0262942.ref005","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/are.13942","article-title":"Effect of probiotics on immune competence of giant freshwater prawn Macrobrachium rosenbergii","volume":"50","author":"MAK Azad","year":"2019","journal-title":"Aquacult. 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