{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T22:54:08Z","timestamp":1771023248744,"version":"3.50.1"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"special funds for discipline construction of gansu agricultural university","award":["No. GAU-XKJS-2018-155"],"award-info":[{"award-number":["No. GAU-XKJS-2018-155"]}]},{"name":"lanzhou science and technology bureau project","award":["2018-4-78"],"award-info":[{"award-number":["2018-4-78"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The aim of this study was to uncover the molecular mechanism through which fungicide resistance develops in <jats:italic>Podosphaera xanthii<\/jats:italic>, a fungi that causes powdery mildew in hull-less pumpkin. Treatments of inoculated <jats:italic>P<\/jats:italic>. <jats:italic>xanthii<\/jats:italic> were carried out on leaves of hull-less pumpkin and subsequently treated with kinds of triazole fungicide for seven generations. Resistant strains of <jats:italic>P<\/jats:italic>. <jats:italic>xanthii<\/jats:italic> thus obtained were evaluated for their resistance levels. The resistance levels of the fungi to four fungicides of were high except that of the propiconazole-resistant strain, which showed moderate resistance. The F7 generations of five resistant strains thus obtained were cultured continuously for five generations without fungicide induction, and their resistance level were found to be relatively stable. The DNA of the sensitive strain and the five kinds of resistant strains were extracted by the sodium dodecyl sulfate (SDS) method and its internal transcribed spacer (ITS) region was amplified by using ITS1\/ITS4 primer and specific primer F\/R and they were sequenced respectively. The DNA sequence comparison of resistant and sensitive strains showed that the base pairs of tebuconazole-resistant strains and flusilazole-resistant strains were mutated, with mutation rates of 4.8% and 1.6%, respectively. The base pairs of the other three resistant strains did not change.<\/jats:p>","DOI":"10.1371\/journal.pone.0263068","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T18:26:04Z","timestamp":1643739964000},"page":"e0263068","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Induction of resistance of Podosphaera xanthii (hull-less pumpkin powdery mildew) to triazole fungicides and its resistance mechanism"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5761-6258","authenticated-orcid":true,"given":"Qiaolan","family":"Liang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liexin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingliang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuwu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"issue":"2","key":"pone.0263068.ref001","first-page":"63","article-title":"Use of Hull-less pumpkin (Cucurbita pepo L.) seed oil press-cake in gluten-free cookies: Nutritional and mineral profile","volume":"28","author":"O Radocaj","year":"2017","journal-title":"Agro Food Industry Hi-Tech"},{"issue":"1","key":"pone.0263068.ref002","doi-asserted-by":"crossref","first-page":"104","DOI":"10.5115\/acb.20.228","article-title":"Anti-proliferative and apoptotic effects of hull-less pumpkin extract on human papillary thyroid carcinoma cell line","volume":"54","author":"HB Mohammad","year":"2021","journal-title":"Anatomy & cell biology"},{"issue":"8","key":"pone.0263068.ref003","first-page":"1095","article-title":"Chemical compositions and mineral contents of some hulless pumpkin seed and oils","volume":"93","author":"S Musa","year":"2015","journal-title":"Journal of the American Oil Chemists\u2019 Society"},{"issue":"8","key":"pone.0263068.ref004","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.3390\/agronomy10081104","article-title":"Seed lipid composition of new hybrids of styrian oil pumpkin grown in Poland","volume":"10","author":"Magorzata Ta\u0144ska","year":"2020","journal-title":"Agronomy"},{"key":"pone.0263068.ref005","doi-asserted-by":"crossref","first-page":"415","DOI":"10.17660\/ActaHortic.2016.1127.64","article-title":"A new pumpkin cultivar \u201cStripe pepo\u201d with hull-less seed and short internodes","volume":"1127","author":"D Kami","year":"2016","journal-title":"Acta Horticulturae"},{"issue":"4","key":"pone.0263068.ref006","first-page":"55","article-title":"Research progress in the functional factors of pumpkin","volume":"20","author":"HW Wang","year":"2004","journal-title":"Food & Machiney"},{"key":"pone.0263068.ref007","unstructured":"Statistics on the total import and export volume and amount of China\u2019s white melon seeds (12129993) from 2014 to 2018. https:\/\/www.chyxx.com."},{"issue":"3","key":"pone.0263068.ref008","first-page":"42","article-title":"Cucurbita\u2014the most Diversity","author":"M. 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