{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:11:12Z","timestamp":1791835872699,"version":"4.3.4"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:00:00Z","timestamp":1659052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["233255\/F50"],"award-info":[{"award-number":["233255\/F50"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Streptococcus agalactiae (group B streptococcus; GBS) is an important human pathogen causing pneumonia, sepsis and meningitis in neonates, as well as infections in pregnant women, immunocompromised individuals, and the elderly. For the future control of GBS-inflicted disease, GBS surface exposed proteins are particularly relevant as they may act as antigens for vaccine development and\/or as serosubtype markers in epidemiological settings. Even so, the genes encoding some of the surface proteins established as serosubtype markers by antibody-based methods, like the R3 surface protein, are still unknown. Here, by examining a Norwegian GBS collection consisting of 140 strains, we find that R3 protein expression correlates with the presence of the gene <jats:italic>sar5<\/jats:italic>. By inducible expression of <jats:italic>sar5<\/jats:italic> in an R3-negative bacterial strain we show that the <jats:italic>sar5<\/jats:italic> gene product is specifically recognized by an R3 monoclonal antibody. With this we identify <jats:italic>sar5<\/jats:italic> as the gene encoding the R3 surface protein, a serosubtype marker of hitherto unknown genetic origin.<\/jats:p>","DOI":"10.1371\/journal.pone.0263199","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T17:39:21Z","timestamp":1659116361000},"page":"e0263199","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Streptococcus agalactiae R3 surface protein is encoded by sar5"],"prefix":"10.1371","volume":"17","author":[{"given":"Adelle","family":"Basson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2266-4150","authenticated-orcid":true,"given":"Camilla","family":"Olaisen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linn-Karina","family":"Selvik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randi Vals\u00f8","family":"Lyng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde","family":"Lysvand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Gidon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9605-3283","authenticated-orcid":true,"given":"Christina Gabrielsen","family":"Aas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2489-4179","authenticated-orcid":true,"given":"Jan Egil","family":"Afset","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1546-4329","authenticated-orcid":true,"given":"Marte Sings\u00e5s","family":"Dragset","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"issue":"suppl_2","key":"pone.0263199.ref001","doi-asserted-by":"crossref","first-page":"S100","DOI":"10.1093\/cid\/cix658","article-title":"Maternal Colonization With Group B Streptococcus and Serotype Distribution Worldwide: Systematic Review and Meta-analyses","volume":"65","author":"NJ Russell","year":"2017","journal-title":"Clin Infect Dis"},{"key":"pone.0263199.ref002","doi-asserted-by":"crossref","first-page":"720789","DOI":"10.3389\/fcimb.2021.720789","article-title":"Bacterial and Host Determinants of Group B Streptococcal Vaginal Colonization and Ascending Infection in Pregnancy","volume":"11","author":"A Brokaw","year":"2021","journal-title":"Frontiers in cellular and infection microbiology"},{"issue":"7","key":"pone.0263199.ref003","doi-asserted-by":"crossref","first-page":"766","DOI":"10.3109\/14767058.2014.934804","article-title":"Intrapartum GBS screening and antibiotic prophylaxis: a European consensus conference","volume":"28","author":"GC Di Renzo","year":"2015","journal-title":"J Matern Fetal Neonatal Med"},{"key":"pone.0263199.ref004","first-page":"204","article-title":"Group B Streptococcus prophylaxis in patients who report a penicillin allergy: a follow-up study","author":"AS Critchfield","year":"2011","journal-title":"Am J Obstet Gynecol"},{"issue":"3","key":"pone.0263199.ref005","doi-asserted-by":"crossref","first-page":"e238","DOI":"10.1055\/s-0039-1694031","article-title":"Intrapartum Group B Streptococcus Antibiotic Prophylaxis in Penicillin Allergic Pregnant Women","volume":"9","author":"N Desravines","year":"2019","journal-title":"Ajp Rep"},{"key":"pone.0263199.ref006","doi-asserted-by":"crossref","DOI":"10.3389\/fped.2018.00027","article-title":"Group B Streptococcal Maternal Colonization and Neonatal Disease: Molecular Mechanisms and Preventative Approaches","volume":"6","author":"KA Patras","year":"2018","journal-title":"Front Pediatr"},{"issue":"1","key":"pone.0263199.ref007","doi-asserted-by":"crossref","first-page":"76","DOI":"10.7774\/cevr.2018.7.1.76","article-title":"Status of group B streptococcal vaccine development","volume":"7","author":"SM Lin","year":"2018","journal-title":"Clin Exp Vaccine Res"},{"issue":"4","key":"pone.0263199.ref008","doi-asserted-by":"crossref","first-page":"313","DOI":"10.4103\/0255-0857.71819","article-title":"Serotype markers in a Streptococcus agalactiae strain collection from Zimbabwe","volume":"28","author":"RT Mavenyengwa","year":"2010","journal-title":"Indian J Med 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