{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:29:23Z","timestamp":1772213363353,"version":"3.50.1"},"reference-count":18,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI20C2557000020"],"award-info":[{"award-number":["HI20C2557000020"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Rapid and accurate detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the successful control of the current global COVID-19 pandemic. The real-time reverse transcription polymerase chain reaction (Real-time RT-PCR) is the most widely used detection technique. This research describes the development of two novel multiplex real-time RT-PCR kits,\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    COVID-19 Multiplex Real-Time RT-PCR Kit (NCVM) specifically designed for use with the\n                    <jats:italic>ExiStation<\/jats:italic>\n                    \u212248 system (comprised of\n                    <jats:italic>ExiPrep<\/jats:italic>\n                    \u212248 Dx and\n                    <jats:italic>Exicycler<\/jats:italic>\n                    \u212296 by BIONEER, Korea) for sample RNA extraction and PCR detection, and\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    SARS-CoV-2 Multiplex Real-Time RT-PCR Kit (SCVM) designed to be compatible with manufacturers\u2019 on-market PCR instruments. The limit of detection (LoD) of NCVM was 120 copies\/mL and the LoD of the SCVM was 2 copies\/\u03bcL for both the Pan-sarbecovirus gene and the SARS-CoV-2 gene. The\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    kits demonstrated high precision with no cross reactivity to other respiratory-related microorganisms. The clinical performance of\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    kits was evaluated using the following clinical samples: sputum and nasopharyngeal\/oropharyngeal swab (NPS\/OPS) samples. Overall agreement of the\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    kits with a Food and Drug Administration (FDA) approved emergency use authorized commercial kit (STANDARD\u2122 M nCoV Real-Time Detection kit, SD BIOSENSOR, Korea) was above 95% (Cohen\u2019s kappa coefficient \u2265 0.95), with a sensitivity of over 95%. The NPS\/OPS specimen pooling experiment was conducted to verify the usability of\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    kits on pooled samples and the results showed greater than 90% agreement with individual NPS\/OPS samples. The clinical performance of\n                    <jats:italic>AccuPower<\/jats:italic>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    kits with saliva samples was also compared with NPS\/OPS samples and demonstrated over 95% agreement (Cohen\u2019s kappa coefficient &gt; 0.95). This study shows the BIONEER NCVM and SCVM assays are comparable with the current standard confirmation assay and are suitable for effective clinical management and control of SARS-CoV-2.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0263341","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T13:47:15Z","timestamp":1644500835000},"page":"e0263341","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and Evaluation of AccuPower COVID-19 Multiplex Real-Time RT-PCR Kit and AccuPower SARS-CoV-2 Multiplex Real-Time RT-PCR Kit for SARS-CoV-2 Detection in Sputum, NPS\/OPS, Saliva and Pooled Samples"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7012-0305","authenticated-orcid":true,"given":"In Bum","family":"Suh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaegyun","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo Seon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guil","family":"Rhim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heebum","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sae-Mi","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun-sang","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Ju","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MyungKook","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyung Sook","family":"Shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4148-9116","authenticated-orcid":true,"given":"Moon Jung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"issue":"7798","key":"pone.0263341.ref001","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2012-7","article-title":"A pneumonia outbreak associated with a new coronavirus of probable bat origin","volume":"579","author":"P Zhou","year":"2020","journal-title":"Nature"},{"issue":"4","key":"pone.0263341.ref002","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/s41591-020-0820-9","article-title":"The proximal origin of SARS-CoV-2","volume":"26","author":"KG Andersen","year":"2020","journal-title":"Nat Med"},{"issue":"10","key":"pone.0263341.ref003","doi-asserted-by":"crossref","first-page":"e3000867","DOI":"10.1371\/journal.pbio.3000867","article-title":"Detection of SARS-CoV-2 RNA by multiplex RT-qPCR","volume":"18","author":"E Kudo","year":"2020","journal-title":"PLoS Biol"},{"key":"pone.0263341.ref004","doi-asserted-by":"crossref","first-page":"104412","DOI":"10.1016\/j.jcv.2020.104412","article-title":"Comparison of seven commercial RT-PCR diagnostic kits for COVID-19.","volume":"128","author":"PB van Kasteren","year":"2020","journal-title":"J Clin Virol"},{"issue":"1","key":"pone.0263341.ref005","doi-asserted-by":"crossref","first-page":"e4","DOI":"10.1002\/viw2.4","article-title":"Promising methods for detection of novel coronavirus SARS\u2010CoV\u20102.","volume":"1","author":"R Liu","year":"2020","journal-title":"View"},{"key":"pone.0263341.ref006","volume-title":"CLSI guideline EP17-A2. 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