{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:16:32Z","timestamp":1747196192686,"version":"3.40.5"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PAPIIT-UNAM","award":["RA-200516"],"award-info":[{"award-number":["RA-200516"]}]},{"name":"PAPIIT-UNAM","award":["RA-200518"],"award-info":[{"award-number":["RA-200518"]}]},{"name":"CONACyT Basic Science","award":["254240"],"award-info":[{"award-number":["254240"]}]},{"name":"UAEM\u00e9x","award":["4668\/2019SF"],"award-info":[{"award-number":["4668\/2019SF"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Non-avian reptiles, unlike mammals and birds, have undergone numerous sex determination changes. Casque-Headed Lizards have replaced the ancestral XY system shared across pleurodonts with a new pair of XY chromosomes. However, the evolutionary forces that triggered this transition have remained unclear. An interesting hypothesis suggests that species with intermediate states, with sex chromosomes but also thermal-induced sex reversal at specific incubation temperatures, could be more susceptible to sex determination turnovers. We contrasted genotypic data (presence\/absence of the Y chromosome) against the histology of gonads of embryos from stages 35\u201337 incubated at various temperatures, including typical male-producing (26\u00b0C) and female-producing (32\u00b0C) temperatures. Our work apparently reports for the first time the histology of gonads, including morphological changes, from stages 35\u201337 of development in the family <jats:italic>Corytophanidae<\/jats:italic>. We also observed that all embryos developed hemipenes, suggesting sex-linked developmental heterochrony. We observed perfect concordance between genotype and phenotype at all temperatures. However, analysis of transcriptomic data from embryos incubated at 26\u00b0C and 32\u00b0C identified transcript variants of the chromatin modifiers <jats:italic>JARID2<\/jats:italic> and <jats:italic>KDM6B<\/jats:italic> that have been linked to temperature-dependent sex determination in other reptiles. Our work tested the validity of a mixed sex determination system in the family <jats:italic>Corytophanidae<\/jats:italic>. We found that XY chromosomes are dominant; however, our work supports the hypothesis of a conserved transcriptional response to incubation temperatures across non-avian reptiles that could be a reminiscence of an ancestral sex determination system.<\/jats:p>","DOI":"10.1371\/journal.pone.0263804","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T17:35:27Z","timestamp":1657215327000},"page":"e0263804","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard"],"prefix":"10.1371","volume":"17","author":[{"given":"Gabriel","family":"Su\u00e1rez-Var\u00f3n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Mendoza-Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Acosta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maricela","family":"Villagr\u00e1n-Santa Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0654-6349","authenticated-orcid":true,"given":"Diego","family":"Cortez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oswaldo","family":"Hern\u00e1ndez-Gallegos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"issue":"3","key":"pone.0263804.ref001","doi-asserted-by":"crossref","first-page":"e59741","DOI":"10.1371\/journal.pone.0059741","article-title":"Global taxonomic diversity of living reptiles.","volume":"8","author":"D Pincheira-Donoso","year":"2013","journal-title":"PLoS One."},{"issue":"7","key":"pone.0263804.ref002","doi-asserted-by":"crossref","first-page":"e1001899","DOI":"10.1371\/journal.pbio.1001899","article-title":"Sex determination: why so many ways of doing it?","volume":"12","author":"D Bachtrog","year":"2014","journal-title":"PLoS Biol"},{"issue":"6","key":"pone.0263804.ref003","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1002\/bies.20050","article-title":"The ends of a continuum: genetic and temperature-dependent sex determination in reptiles","volume":"26","author":"SD Sarre","year":"2004","journal-title":"Bioessays"},{"issue":"4","key":"pone.0263804.ref004","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1046\/j.1461-0248.2002.00351.x","article-title":"Co\u2010occurrence of multiple, supposedly incompatible modes of sex determination in a lizard population","volume":"5","author":"R Shine","year":"2002","journal-title":"Ecology Letters"},{"issue":"1\u20133","key":"pone.0263804.ref005","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1159\/000515687","article-title":"Temperature-Induced Sex Reversal in Reptiles: Prevalence, Discovery, and Evolutionary Implications.","volume":"15","author":"SL Whiteley","year":"2021","journal-title":"Sex Dev."},{"issue":"4","key":"pone.0263804.ref006","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1093\/gbe\/evy042","article-title":"Conservation of Sex-Linked Markers among Conspecific Populations of a Viviparous Skink, Niveoscincus ocellatus, Exhibiting Genetic and Temperature-Dependent Sex Determination","volume":"10","author":"PL Hill","year":"2018","journal-title":"Genome Biol Evol"},{"issue":"6848","key":"pone.0263804.ref007","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/35089135","article-title":"Sex determination. 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