{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:30:06Z","timestamp":1785526206560,"version":"3.56.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T00:00:00Z","timestamp":1644883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland","award":["SN\/GW08\/2020"],"award-info":[{"award-number":["SN\/GW08\/2020"]}]},{"name":"Count Jakub Potocki\u2019s Foudation, Warsaw, Poland","award":["825\/18"],"award-info":[{"award-number":["825\/18"]}]},{"DOI":"10.13039\/501100011089","name":"PLGrid Infrastructure","doi-asserted-by":"crossref","award":["ovcarnaseq"],"award-info":[{"award-number":["ovcarnaseq"]}],"id":[{"id":"10.13039\/501100011089","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The occurrence of<jats:italic>MYC<\/jats:italic>-negative Burkitt lymphoma (BL) has been discussed for many years. The real frequency of the<jats:italic>MYC<\/jats:italic>insertion in<jats:italic>MYC<\/jats:italic>-negative BL is still unknown. Fine-needle aspiration biopsies of 108 consecutive patients with clinicopathologically suspected BL (suspBL) were evaluated by flow cytometry, classical cytogenetics, and fluorescence in situ hybridization (FISH). We found 12 cases (11%) without the<jats:italic>MYC<\/jats:italic>rearrangement by FISH with a MYC breakapart probe: two patients (1.9%) with cryptic<jats:italic>MYC\/IGH<\/jats:italic>fusion (finally diagnosed as BL) and 10 patients (9.3%) with 11q gain\/loss (finally diagnosed as Burkitt-like lymphoma with 11q aberration). The exact breakpoints of the cryptic<jats:italic>MYC\/IGH<\/jats:italic>were investigated by next-generation sequencing. The<jats:italic>MYC<\/jats:italic>insertions\u2019 breakpoints were identified in<jats:italic>PVT1<\/jats:italic>in the first case, and 42 kb upstream of 5\u2032<jats:italic>MYC<\/jats:italic>in the second case. To date, a molecular characterization of the<jats:italic>MYC<\/jats:italic>insertion in BL has only been reported in one case. Detailed descriptions of our<jats:italic>MYC<\/jats:italic>insertions in a routinely and consecutively diagnosed suspBL cohort will contribute to resolving the issue of<jats:italic>MYC<\/jats:italic>negativity in BL. In our opinion, the presence of the<jats:italic>MYC<\/jats:italic>insertions in BL and other lymphomas might be underestimated, because routine genetic diagnostics are usually based on FISH only, without karyotyping.<\/jats:p>","DOI":"10.1371\/journal.pone.0263980","type":"journal-article","created":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T18:29:12Z","timestamp":1644949752000},"page":"e0263980","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2531-2775","authenticated-orcid":true,"given":"Renata","family":"Woroniecka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Rymkiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1670-0274","authenticated-orcid":true,"given":"Lukasz M.","family":"Szafron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Blachnio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura 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