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We optimized a sample-to-answer nucleic acid test for SARS-CoV-2 that provides results in &lt;1 hour using inexpensive and readily available reagents. The test workflow includes a simple lysis and viral inactivation protocol followed by direct isothermal amplification of viral RNA using RT-LAMP. The assay was validated using two different instruments, a portable isothermal fluorimeter and a standard thermocycler. Results of the RT-LAMP assay were compared to traditional RT-qPCR for nasopharyngeal swabs, nasal swabs, and saliva collected from a cohort of patients hospitalized due to COVID-19. For all three sample types, positive agreement with RT-LAMP performed using the isothermal fluorimeter was 100% for samples with Ct &lt;30 and 69\u201391% for samples with Ct &lt;40. Following validation, the test was successfully scaled to test the saliva of up to 400 asymptomatic individuals per day as part of the campus surveillance program at Rice University. Successful development, validation, and scaling of this sample-to-answer, extraction-free real-time RT-LAMP test for SARS-CoV-2 adds a highly adaptable tool to efforts to control the COVID-19 pandemic, and can inform test development strategies for future infectious disease threats.<\/jats:p>","DOI":"10.1371\/journal.pone.0264130","type":"journal-article","created":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T18:45:06Z","timestamp":1645814706000},"page":"e0264130","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Sample-to-answer, extraction-free, real-time RT-LAMP test for SARS-CoV-2 in nasopharyngeal, nasal, and saliva samples: Implications and use for surveillance testing"],"prefix":"10.1371","volume":"17","author":[{"given":"Kathryn A.","family":"Kundrod","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary 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Schermer","year":"2020","journal-title":"PLoS One"},{"key":"pone.0264130.ref030","unstructured":"Cairns E. 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Optimized Integration of New England Biolabs \u00ae Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments."},{"key":"pone.0264130.ref038","doi-asserted-by":"crossref","first-page":"112592","DOI":"10.1016\/j.bios.2020.112592","article-title":"Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases","volume":"169","author":"R Paul","year":"2020","journal-title":"Biosens Bioelectron"},{"key":"pone.0264130.ref039","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ijms21228741","article-title":"Fast, precise, and reliable multiplex detection of potato viruses by loop-mediated isothermal amplification","volume":"21","author":"G Edg\u00fc","year":"2020","journal-title":"Int J Mol Sci"},{"key":"pone.0264130.ref040","doi-asserted-by":"crossref","first-page":"e76","DOI":"10.1056\/NEJMvcm2010260","article-title":"How to Obtain a Nasopharyngeal Swab Specimen","volume":"382","author":"FM Marty","year":"2020","journal-title":"N Engl J Med"},{"key":"pone.0264130.ref041","unstructured":"CLIA University Lab Testing. 2020. https:\/\/www.cms.gov\/files\/document\/clia-university-lab-testing.pdf"},{"key":"pone.0264130.ref042","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.jbi.2008.08.010","article-title":"Research electronic data capture (REDCap)\u2014A metadata-driven methodology and workflow process for providing translational research informatics support","volume":"42","author":"PA Harris","year":"2009","journal-title":"J Biomed Inform"},{"key":"pone.0264130.ref043","doi-asserted-by":"crossref","first-page":"103208","DOI":"10.1016\/j.jbi.2019.103208","article-title":"The REDCap consortium: Building an international community of software platform partners","volume":"95","author":"PA Harris","year":"2019","journal-title":"J Biomed 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