{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:29:29Z","timestamp":1787956169294,"version":"build-2784847793"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L020513\/1"],"award-info":[{"award-number":["BB\/L020513\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/P0257731"],"award-info":[{"award-number":["BB\/P0257731"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Northwest cancer research"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Laminin N-terminus \u03b131 (LaNt \u03b131) is an alternative splice isoform derived from the laminin \u03b13 gene. The LaNt \u03b131 protein is enriched around the terminal duct lobular units in normal breast tissue. In the skin and cornea the protein influences epithelial cell migration and tissue remodelling. However, LaNt \u03b131 has never been investigated in a tumour environment. Here we analysed LaNt \u03b131 in invasive ductal carcinoma and determined its contribution to breast carcinoma invasion. LaNt \u03b131 expression and distribution were analysed by immunohistochemistry in human breast tissue biopsy sections and tissue microarrays covering 232 breast cancer samples. This analysis revealed LaNt \u03b131 to be upregulated in 56% of invasive ductal carcinoma specimens compared with matched normal tissue, and further increased in nodal metastasis compared with the tumour mass in 45% of samples. 65.8% of triple negative cases displayed medium to high LaNt \u03b131 expression. To study LaNt \u03b131 function, an adenoviral system was used to induce expression in MCF-7 and MDA-MB-231 cells. 2D cell migration and invasion into collagen hydrogels were not significantly different between LaNt \u03b131 overexpressing cells and control treated cells. However, LaNt \u03b131 overexpression reduced the proliferation rate of MCF-7 and MDA-MB-231 cells. Moreover, LaNt \u03b131 overexpressing MDA-MB-231 cells displayed a striking change in their mode of invasion into laminin-containing Matrigel; changing from multicellular streaming to individual cellular-invasion. In agreement with these results, 66.7% of the tumours with the highest LaNt \u03b131 expression were non-cohesive. Together these findings indicate that breast cancer-associated changes in LaNt \u03b131 expression could contribute to the processes involved in tumour invasion and may represent a new therapeutic target.<\/jats:p>","DOI":"10.1371\/journal.pone.0264430","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T13:31:45Z","timestamp":1646141505000},"page":"e0264430","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Laminin N-terminus \u03b131 is upregulated in invasive ductal breast cancer and changes the mode of tumour invasion"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3836-7249","authenticated-orcid":true,"given":"Lee D.","family":"Troughton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9313-8433","authenticated-orcid":true,"given":"Danielle A.","family":"O\u2019Loughlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Zech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Hamill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,3,1]]},"reference":[{"issue":"1","key":"pone.0264430.ref001","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0092-8674(00)81683-9","article-title":"The hallmarks of cancer","volume":"100","author":"D Hanahan","year":"2000","journal-title":"Cell"},{"issue":"1","key":"pone.0264430.ref002","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s005990050058","article-title":"Evaluation of serum laminin as a tumor marker in breast cancer","volume":"29","author":"G Sidhom","year":"1999","journal-title":"Int J Clin Lab Res"},{"issue":"8","key":"pone.0264430.ref003","doi-asserted-by":"crossref","first-page":"6280","DOI":"10.1002\/jcp.26513","article-title":"Extracellular matrix proteins as diagnostic markers of breast carcinoma","volume":"233","author":"M Giussani","year":"2018","journal-title":"J Cell Physiol"},{"issue":"24","key":"pone.0264430.ref004","doi-asserted-by":"crossref","first-page":"e1800278","DOI":"10.1002\/pmic.201800278","article-title":"ECM Remodeling in Breast Cancer with Different Grade: Contribution of 2D-DIGE Proteomics","volume":"18","author":"M Moriggi","year":"2018","journal-title":"Proteomics"},{"issue":"4","key":"pone.0264430.ref005","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/bcr1270","article-title":"Laminin isoform expression in breast tumors","volume":"7","author":"E. 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