{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:59:09Z","timestamp":1790351949298,"version":"4.1.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and infectious disease","doi-asserted-by":"crossref","award":["U01HG007480, U54HG007480"],"award-info":[{"award-number":["U01HG007480, U54HG007480"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004421","name":"World Bank Group","doi-asserted-by":"publisher","award":["ACE IMPACT"],"award-info":[{"award-number":["ACE IMPACT"]}],"id":[{"id":"10.13039\/100004421","id-type":"DOI","asserted-by":"publisher"}]},{"name":"U.S President\u2019s Malaria Initiative"},{"name":"TED's Audacious Project"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Accurate assessment and monitoring of the<jats:italic>Plasmodium falciparum<\/jats:italic>Kelch 13 (<jats:italic>pfk13)<\/jats:italic>gene associated with artemisinin resistance is critical to understand the emergence and spread of drug-resistant parasites in malaria-endemic regions. In this study, we evaluated the genomic profile of the<jats:italic>pfk13<\/jats:italic>gene associated with artemisinin resistance in<jats:italic>P<\/jats:italic>.<jats:italic>falciparum<\/jats:italic>in Nigerian children by targeted sequencing of the<jats:italic>pfk13<\/jats:italic>gene. Genomic DNA was extracted from 332 dried blood (DBS) spot filter paper samples from three Nigerian States. The<jats:italic>pfk13<\/jats:italic>gene was amplified by nested polymerase chain reaction (PCR), and amplicons were sequenced to detect known and novel polymorphisms across the gene. Consensus sequences of samples were mapped to the reference gene sequence obtained from the National Center for Biotechnology Information (NCBI). Out of the 13 single nucleotide polymorphisms (SNPs) detected in the<jats:italic>pfk13<\/jats:italic>gene, five (F451L, N664I, V487E, V692G and Q661H) have not been reported in other endemic countries to the best of our knowledge. Three of these SNPs (V692G, N664I and Q661H) and a non-novel SNP, C469C, were consistent with late parasitological failure (LPF) in two States (Enugu and Plateau States). There was no validated mutation associated with artemisinin resistance in this study. However, a correlation of our study with<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>phenotypes is needed to establish the functional role of detected mutations as markers of artemisinin resistance in Nigeria. This baseline information will be essential in tracking and monitoring<jats:italic>P<\/jats:italic>.<jats:italic>falciparum<\/jats:italic>resistance to artemisinin in Nigeria.<\/jats:p>","DOI":"10.1371\/journal.pone.0264548","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T18:26:27Z","timestamp":1646072787000},"page":"e0264548","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["Molecular profiling of the artemisinin resistance Kelch 13 gene in Plasmodium falciparum from Nigeria"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3149-3085","authenticated-orcid":true,"given":"Fehintola V.","family":"Ajogbasile","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2651-2149","authenticated-orcid":true,"given":"Paul E.","family":"Oluniyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4249-9850","authenticated-orcid":true,"given":"Adeyemi T.","family":"Kayode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazeem O.","family":"Akano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin B.","family":"Adegboyega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courage","family":"Philip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nnenna","family":"Ogbulafor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrietta U.","family":"Okafor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Oguche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robinson D.","family":"Wammanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olugbenga A.","family":"Mokuolu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onikepe A.","family":"Folarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3056-6705","authenticated-orcid":true,"given":"Christian T.","family":"Happi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"issue":"8","key":"pone.0264548.ref001","first-page":"6","article-title":"Evolution of Drug Resistance in Malaria Parasite Populations","volume":"4","author":"Y Kim","year":"2013","journal-title":"Nat Ed Knowledge"},{"key":"pone.0264548.ref002","unstructured":"World Health Organization, Report on antimalarial drug efficacy, resistance and response: 10years of surveillance (2010\u20132019), World Health Organization, 2020."},{"issue":"5","key":"pone.0264548.ref003","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1056\/NEJMoa0808859","article-title":"Artemisinin resistance in Plasmodium falciparum malaria","volume":"361","author":"AM Dondorp","year":"2009","journal-title":"N Engl J Med"},{"issue":"24","key":"pone.0264548.ref004","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1056\/NEJMc0805011","article-title":"Artemisinin Resistance in Cambodia 1 (ARC1) Study Consortium. 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