{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T23:22:21Z","timestamp":1778628141382,"version":"3.51.4"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Shanxi Province of China","award":["201901D111326"],"award-info":[{"award-number":["201901D111326"]}]},{"name":"Natural Science Foundation of Shanxi Province of China","award":["20210302124299"],"award-info":[{"award-number":["20210302124299"]}]},{"name":"Academic Technology Leader Project of Changzhi Medical College","award":["XS202103"],"award-info":[{"award-number":["XS202103"]}]},{"name":"Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi","award":["2020L0376"],"award-info":[{"award-number":["2020L0376"]}]},{"name":"Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi","award":["2020L0377"],"award-info":[{"award-number":["2020L0377"]}]},{"name":"Undergraduate Innovation and Entrepreneurship Training Program","award":["20210584"],"award-info":[{"award-number":["20210584"]}]},{"name":"Major Infectious Diseases Such as AIDS and Viral Hepatitis Prevention and Control Technology Major Projects","award":["2018ZX10712001"],"award-info":[{"award-number":["2018ZX10712001"]}]},{"name":"Major Infectious Diseases Such as AIDS and Viral Hepatitis Prevention and Control Technology Major Projects","award":["2017ZX10303404"],"award-info":[{"award-number":["2017ZX10303404"]}]},{"name":"Doctor research startup foundation of Changzhi Medical College","award":["BS201912"],"award-info":[{"award-number":["BS201912"]}]},{"name":"Doctor research startup foundation of Changzhi Medical College","award":["BS201921"],"award-info":[{"award-number":["BS201921"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The prevalence and molecular characteristics of <jats:italic>Bartonella<\/jats:italic> infections in small rodents in the Zhongtiao Mountain, China have been explored. In this study, the liver, spleen and kidney tissues of captured rodents were used for <jats:italic>Bartonella spp<\/jats:italic>. detection and identification by combination of real-time PCR of transfer-mRNA (<jats:italic>ssrA<\/jats:italic>) gene and traditional PCR and sequencing of citrate synthase (<jats:italic>gltA<\/jats:italic>) gene. It was shown that 49.52% of the rodents (52\/105) were positive for <jats:italic>Bartonella spp<\/jats:italic>.. The infection rate in different gender (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 0.079, <jats:italic>P<\/jats:italic> = 0.778) and tissues (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 0.233, <jats:italic>P<\/jats:italic> = 0.890) of small rodents did not have statistical difference, but that in different small rodents (Fisher\u2019s exact test, <jats:italic>P<\/jats:italic> &lt; 0.001) and habitats (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 5.483, <jats:italic>P<\/jats:italic> = 0.019) had statistical difference. And, the sequencing data suggests that <jats:italic>Bartonella<\/jats:italic> sequences (n = 31) were identified into three species, including 14 of <jats:italic>B<\/jats:italic>. <jats:italic>grahamii<\/jats:italic>, 3 of <jats:italic>B<\/jats:italic>. <jats:italic>queenslandensis<\/jats:italic> and 14 of <jats:italic>unknown Bartonella<\/jats:italic> species. Phylogenetic analysis showed that <jats:italic>B<\/jats:italic>. <jats:italic>grahamii<\/jats:italic> sequences were clustered with the isolates from South Korea and China, and <jats:italic>B<\/jats:italic>. <jats:italic>queenslandensis<\/jats:italic> sequences were mainly closely related to the isolates from China and Thailand. The genetic diversity analysis showed that <jats:italic>B<\/jats:italic>. <jats:italic>grahamii<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>queenslandensis<\/jats:italic> sequences exhibited noticeable intraspecies diversity. Taken together our data demonstrates the high prevalence and genetic diversity of <jats:italic>Bartonella<\/jats:italic> infections in small rodents in the Zhongtiao Mountain, especially a potential novel <jats:italic>Bartonella<\/jats:italic> specie was detected, which could benefit the prevention and control of rodent-<jats:italic>Bartonella<\/jats:italic> species in this area.<\/jats:p>","DOI":"10.1371\/journal.pone.0264591","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T18:22:45Z","timestamp":1646072565000},"page":"e0264591","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China"],"prefix":"10.1371","volume":"17","author":[{"given":"Juan","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiong-Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Xia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Bing","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Mei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6388-8276","authenticated-orcid":true,"given":"Hua-Xiang","family":"Rao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"issue":"3","key":"pone.0264591.ref001","doi-asserted-by":"crossref","first-page":"389","DOI":"10.3201\/eid1203.050931","article-title":"Bartonella 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