{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:54:16Z","timestamp":1791485656500,"version":"4.3.4"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T00:00:00Z","timestamp":1651536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"Staphylococcus aureus<\/jats:italic> (S<\/jats:italic>. aureus<\/jats:italic>) is the most prevalent microorganism associated with mastitis in cattle, which harbours several virulence factors and antibiotic resistance genes. The present study aimed to characterize S<\/jats:italic>. aureus<\/jats:italic> isolated from mastitic milk of the cattle for antibiotic resistance (blaZ<\/jats:italic> and mecA)<\/jats:italic>, haemolysins (hla<\/jats:italic> and hlb<\/jats:italic>) and enterotoxins (sea<\/jats:italic>, seb<\/jats:italic>, sec<\/jats:italic>, and sed<\/jats:italic>) genes. A total of 69 staphylococci were isolated and phenotypically characterized for haemolytic properties on 5% sheep blood agar medium. Out of 69 isolates, 55 (79.71%) were identified as S<\/jats:italic>. aureus<\/jats:italic> by polymerase chain reaction assay. Among S<\/jats:italic>. aureus<\/jats:italic>, the majority of the isolates harboured the gene blaZ<\/jats:italic> (92.73%), followed by coa<\/jats:italic> (89.09%), hlb<\/jats:italic> (60%) and hla<\/jats:italic> (49.09%). Gene mecA<\/jats:italic> responsible for methicillin resistance was detected in 23.64% of S<\/jats:italic>. aureus<\/jats:italic> isolates. Enterotoxin genes seb<\/jats:italic> (9.09%), sec<\/jats:italic> (1.82%) and sed<\/jats:italic> (7.27%) responsible for food poisoning were detected at a comparatively lower rate and none of the S<\/jats:italic>. aureus<\/jats:italic> strain was found positive for sea<\/jats:italic>. Additionally, antimicrobial susceptibility study of S<\/jats:italic>. aureus<\/jats:italic> against 18 antimicrobial discs showed maximum resistance to oxytetracycline, penicillin, and fluoroquinolone groups, contrarily, we observed maximum sensitivity to methicillin and cefuroxime antimicrobials. The high occurrence rate of S<\/jats:italic>. aureus<\/jats:italic> harbouring genes for virulence factors and antimicrobial resistance needs appropriate strategies to control the pathogen spread to the human population.<\/jats:p>","DOI":"10.1371\/journal.pone.0264762","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T17:28:00Z","timestamp":1651598880000},"page":"e0264762","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":51,"title":["Virulence and antimicrobial resistance gene profiles of Staphylococcus aureus associated with clinical mastitis in cattle"],"prefix":"10.1371","volume":"17","author":[{"family":"Neelam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. 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