{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T17:52:00Z","timestamp":1767894720965,"version":"3.49.0"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004457","name":"The World Academy of Sciences","doi-asserted-by":"publisher","award":["3240315420"],"award-info":[{"award-number":["3240315420"]}],"id":[{"id":"10.13039\/100004457","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Staphylococcus aureus (S<\/jats:italic>. <jats:italic>aureus)<\/jats:italic> is one of the earliest pathogens involved in human infections, responsible for a large variety of pathologies. Methicillin was the first antibiotic used to treat infections due to <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> but infections due to Methicillin resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) originated from hospital settings. Later, severe infections due to MRSA without any contact with the hospital environment or health care workers arose. Prevalence of MRSA has shown an alarming increase worldover including Cameroon. This Cross-sectional study was designed to evaluate the occurrence of MRSA infections in five different, most frequented Hospitals in northern Cameroon. Socio demographic data was recorded through questionnaire and different clinical specimens were collected for bacterial isolation. Identification of <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> was confirmed via 16s rRNA amplification using <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> specific primers. Molecular characterisation was performed through <jats:italic>mecA<\/jats:italic> gene, <jats:italic>Luk PV<\/jats:italic> gene screening and SCCmec typing. A total of 380 <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> clinical isolates were obtained of which 202 (53.2%) were nonduplicate multidrug resistant isolates containing, 45.5% MRSA. Higher number of MRSA was isolated from pus (30.4%) followed by blood culture (18.5%), and urine (17.4%). Patients aged 15 to 30 years presented high prevalence of MRSA (30.4%). Majority isolates (97.8%) carried the m<jats:italic>ecA<\/jats:italic> gene, PVL toxin screening indicated 53.3% isolates carried the <jats:italic>lukPV<\/jats:italic> gene. Based on PVL detection and clinical history, CA-MRSA represented 53.3% of isolates. SCCmec typing showed that the Type IV was most prevalent (29.3%), followed by type I (23.9%). Amongst MRSA isolates high resistance to penicillin (91.1%), cotrimoxazole (86.7%), tetracycline (72.2%), and ofloxacin (70.0%) was detected. Meanwhile, rifampicin, fusidic acid, lincomycin and minocycline presented high efficacy in bacterial control. This study revealed a high prevalence of MRSA among infections due to <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> in Northern Cameroon. All MRSA recorded were multidrug resistant and the prevalence of CA MRSA are subsequently increasing, among population.<\/jats:p>","DOI":"10.1371\/journal.pone.0265118","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T17:49:39Z","timestamp":1657302579000},"page":"e0265118","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["High prevalence of Panton-Valentine leukocidin positive, multidrug resistant, Methicillin-resistant Staphylococcus aureus strains circulating among clinical setups in Adamawa and Far North regions of Cameroon"],"prefix":"10.1371","volume":"17","author":[{"given":"Mansour","family":"Mohamadou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah Riwom","family":"Essama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie Chantal","family":"Ngonde Essome","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lillian","family":"Akwah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nudrat","family":"Nadeem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hortense","family":"Gonsu Kamga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadia","family":"Sattar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8352-8078","authenticated-orcid":true,"given":"Sundus","family":"Javed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"pone.0265118.ref001","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/S0190-9622(89)70035-9","article-title":"Micrococci and Joseph Lister.","volume":"20","author":"A. 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