{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:40:15Z","timestamp":1791945615399,"version":"4.3.4"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T00:00:00Z","timestamp":1649030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Technical co-operation by the International Atomic Energy Agency","award":["TC-SRL5\/047, INT5155, RAS-50\/82"],"award-info":[{"award-number":["TC-SRL5\/047, INT5155, RAS-50\/82"]}]},{"name":"Funding by the National Research Council, Sri Lanka","award":["TO 14-04"],"award-info":[{"award-number":["TO 14-04"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>The Sterile Insect Technique (SIT) is presently being tested to control dengue in several countries. SIT aims to cause the decline of the target insect population through the release of a sufficient number of sterilized male insects. This induces sterility in the female population, as females that mate with sterilized males produce no offspring. Male insects are sterilized through the use of ionizing irradiation. This study aimed to evaluate variable parameters that may affect irradiation in mosquito pupae.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methods<\/jats:title><jats:p>An<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>colony was maintained under standard laboratory conditions. Male and female<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>pupae were separated using a Fay and Morlan glass sorter and exposed to different doses of gamma radiation (40, 50, 60, 70 and 80 Gy) using a Co<jats:sup>60<\/jats:sup>source. The effects of radiation on survival, flight ability and the reproductive capacity of<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>were evaluated under laboratory conditions. In addition, mating competitiveness was evaluated for irradiated male<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>mosquitoes to be used for future SIT programmes in Sri Lanka.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Results<\/jats:title><jats:p>Survival of irradiated pupae was reduced by irradiation in a dose-dependent manner but it was invariably greater than 90% in control, 40, 50, 60, 70 Gy in both male and female<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>. Irradiation didn\u2019t show any significant adverse effects on flight ability of male and female mosquitoes, which consistently exceeded 90%. A similar number of eggs per female was observed between the non-irradiated groups and the irradiated groups for both irradiated males and females. Egg hatch rates were significantly lower when an irradiation dose above 50 Gy was used as compared to 40 Gy in both males and females. Irradiation at higher doses significantly reduced male and female survival when compared to the non-irradiated<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>mosquitoes. Competitiveness index (C) scores of sterile and non-sterile males compared with non-irradiated male mosquitoes under laboratory and semi-field conditions were 0.56 and 0.51 respectively at 50 Gy.<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Signification<\/jats:title><jats:p>Based on the results obtained from the current study, a 50 Gy dose was selected as the optimal radiation dose for the production of sterile<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>males for future SIT-based dengue control programmes aiming at the suppression of<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>populations in Sri Lanka.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pone.0265244","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T17:27:21Z","timestamp":1649093241000},"page":"e0265244","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":37,"title":["Development of the Sterile Insect Technique to control the dengue vector Aedes aegypti (Linnaeus) in Sri Lanka"],"prefix":"10.1371","volume":"17","author":[{"given":"Tharaka","family":"Ranathunge","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeevanie","family":"Harishchandra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamidou","family":"Maiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Bouyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. 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