{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:06:08Z","timestamp":1768457168569,"version":"3.49.0"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"Defense Health Agency Immunization Healthcare Branch","award":["IHBG-15-07"],"award-info":[{"award-number":["IHBG-15-07"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Traditional blood sampling by venipuncture is cumbersome and relatively expensive. Dried blood spot (DBS) sampling is desirable because of its ease of sample collection, transportation and storage. It has been used in clinical diagnosis but not been thoroughly studied for the potential use to assess the immune status of individuals following natural infection or preventive vaccination. The goal of this study was to compare DBS to traditional blood samplings in detection of antibodies in individuals vaccinated against measles, hepatitis A, tetanus, influenza and varicella zoster. Enzyme linked immunosorbent assay (ELISA) was used to test DBS eluates and serum samples for antibodies against measles, varicella, tetanus and hepatitis A. Sensitivities, specificities, and correlation coefficients were evaluated to compare optical density (OD) values of paired serum and DBS samples. The long-term stability of DBS samples at different temperatures was assessed using simulated immune measles blood. DBS OD was highly correlated with serum OD for antibodies to measles (r = 0.93), varicella (r = 0.82), and tetanus (r = 0.91). Sensitivities of DBS OD ranged from 86\u201399% and specificities ranged from 96\u2013100% using cut-offs established by each assay. By contrast, the hepatitis A data showed a low sensitivity (31%) and weak correlation (r = 0.14) between DBS and serum samples. Antibody titers in serum samples for anti-influenza A (H1N1 and H3N1) failed to correlate in DBS eluates in HAI and MN assays. DBS samples were stable for 4 weeks when stored at room temperature and for 6 months at 4\u00b0C. DBS sampling was sensitive, specific, and highly correlated with traditional venipuncture sampling in detection of antibodies against measles, tetanus and varicella zoster, but not hepatitis A and influenza. Thus, the success of using DBS sampling to assess the antibody levels in immunized individuals may be dependent on the pathogens and the development of the assay used.<\/jats:p>","DOI":"10.1371\/journal.pone.0265813","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T21:45:31Z","timestamp":1648158331000},"page":"e0265813","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["The use of dried blood spot cards to assess serologic responses of individuals vaccinated against measles, hepatitis A, tetanus, influenza and varicella zoster"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-0242","authenticated-orcid":true,"given":"Brian","family":"Reinhardt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Dawkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taylor","family":"Banks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora","family":"Watson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Appavu","family":"Sundaram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Ewing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6095-3212","authenticated-orcid":true,"given":"Janine Ruth","family":"Danko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,3,24]]},"reference":[{"key":"pone.0265813.ref001","volume-title":"Surveillance Guidelines for Measles, Rubella and Congenital Rubella Syndrome in the WHO European Region","year":"2012"},{"issue":"3","key":"pone.0265813.ref002","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.jiph.2017.09.014","article-title":"Dried blood spots: An evaluation of utility in the field","volume":"11","author":"X Su","year":"2018","journal-title":"J Infect Public Health"},{"issue":"3","key":"pone.0265813.ref003","first-page":"104","article-title":"Screening for phenylketonuria","volume":"9","author":"R. Guthrie","year":"1969","journal-title":"Triangle"},{"key":"pone.0265813.ref004","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3389\/fmicb.2020.00373","article-title":"Dried Blood Spot Tests for the Diagnosis and 5. Therapeutic Monitoring of HIV and Viral Hepatitis B and C","volume":"11","author":"E Tuaillon","year":"2020","journal-title":"Front Microbiol"},{"issue":"8","key":"pone.0265813.ref005","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1093\/cid\/cis015","article-title":"Dried blood spot specimens are a suitable alternative sample type for HIV-1 viral load measurement and drug resistance genotyping in patients receiving first-line antiretroviral therapy","volume":"54","author":"EK Rottinghaus","year":"2012","journal-title":"Clin Infect Dis"},{"key":"pone.0265813.ref006","first-page":"1","volume":"213","author":"A Soulier","year":"2015","journal-title":"Dried Blood Spots: A Tool to Ensure Broad Access to Hepatitis C Screening, Diagnosis, and Treatment Monitoring"},{"issue":"9","key":"pone.0265813.ref007","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/S1473-3099(15)00226-1","article-title":"Dried blood spot for Ebola testing in developing countries","volume":"15","author":"S Sarkar","year":"2015","journal-title":"Lancet Infect Dis"},{"issue":"12","key":"pone.0265813.ref008","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1002\/jmv.25275","article-title":"Assessing hepatitis B immunity using dried blood spot samples from HIV+ individuals","volume":"90","author":"GL Flores","year":"2018","journal-title":"J Med Virol"},{"key":"pone.0265813.ref009","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jcv.2014.01.014","article-title":"Comparison of Hepatitis C Virus RNA and antibody detection in dried blood spots and plasma specimens","volume":"59","author":"EK Dokubo","year":"2014","journal-title":"J Clin Virol"},{"key":"pone.0265813.ref010","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1002\/jmv.23874","article-title":"Assessment of Dried Blood Samples as an Alternative Less Invasive Method for Detection of Hepatitis E Virus Marker in an Outbreak Setting","volume":"86","author":"MP Singh","year":"2014","journal-title":"J Med Virol"},{"key":"pone.0265813.ref011","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/978-1-4939-3292-4_8","volume-title":"Poliovirus: Methods and Protocols","author":"WC. Weldon","year":"2016"},{"issue":"1","key":"pone.0265813.ref012","doi-asserted-by":"crossref","first-page":"3857","DOI":"10.1038\/s41598-020-60703-1","article-title":"Evaluation of the efficiency of dried blood spot-based measurement of hepatitis B and hepatitis C virus seromarkers","volume":"10","author":"C Yamamoto","year":"2020","journal-title":"Sci Rep"},{"issue":"17","key":"pone.0265813.ref013","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.4155\/bio.13.175","article-title":"State-of-the-art dried blood spot analysis: an overview of recent advances and future trends","volume":"5","author":"RJ Meesters","year":"2013","journal-title":"Bioanalysis"},{"key":"pone.0265813.ref014","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/QAI.0b013e3181edaaf5","article-title":"Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso","volume":"55","author":"J Viljoen","year":"2010","journal-title":"J Acquir Immune Defic Syndr"},{"key":"pone.0265813.ref015","first-page":"e50973","article-title":"Dried blood spot collection of health biomarkers to maximize participation in population studies","volume":"83","author":"MW Ostler","year":"2014","journal-title":"J Vis Exp"},{"issue":"9","key":"pone.0265813.ref016","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1002\/jmv.22138","article-title":"Assessment of dried blood spot samples as a simple method for detection of hepatitis B virus markers","volume":"83","author":"LM Villar","year":"2011","journal-title":"J Med Virol"},{"issue":"12","key":"pone.0265813.ref017","doi-asserted-by":"crossref","first-page":"e48229","DOI":"10.1371\/journal.pone.0048229","article-title":"Comparative analysis of hemagglutination inhibition titers generated using temporally matched serum and plasma samples","volume":"7","author":"GN Defang","year":"2012","journal-title":"PLoS One"},{"key":"pone.0265813.ref018","unstructured":"World Health Organization. WHO manual on animal influenza diagnosis and surveillance. http:\/\/www.who.int\/csr\/resources\/publications\/influenza\/whocdscsrncs20025rev.pdf. Accessed 8 Nov 2017"},{"key":"pone.0265813.ref019","first-page":"701","article-title":"Dried venous blood samples for the detection and quantification of measles IgG using a commercial enzyme immunoassay","volume":"81","author":"Riddell","year":"2003","journal-title":"Bull World Health Organ"},{"key":"pone.0265813.ref020","volume-title":"RStudio: Integrated Development Environment for R","author":"RStudio Team","year":"2015"},{"issue":"1","key":"pone.0265813.ref021","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0166-0934(94)90052-3","article-title":"Detection of Immunoglobulin G to Measles Virus, Rubella Virus, and Mumps Virus in Serum Samples and in Microquantities of Whole Blood Dried on Filter Paper","volume":"49","author":"F Condorelli","year":"1994","journal-title":"J Virol Methods"},{"issue":"2","key":"pone.0265813.ref022","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1089\/bio.2020.0131","article-title":"Mind the Quality Gap When Banking on Dry Blood Spots","volume":"19","author":"D Carpentieri","year":"2021","journal-title":"Biopreservation and Biobanking"},{"issue":"9","key":"pone.0265813.ref023","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1002\/jmv.24136","article-title":"Use of a Commercial ELISA for the Detection of Measles-Specific Immunoglobulin G (IgG) in Dried Blood Spots Collected From Children Living in Low-Resource Settings","volume":"87","author":"KE Colson","year":"2015","journal-title":"Med Virol"},{"issue":"4","key":"pone.0265813.ref024","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1002\/jmv.2100","article-title":"Comparative detection of measles and rubella IgM and IgG derived from filter paper blood and serum samples","volume":"65","author":"RF Helfand","year":"2001","journal-title":"J Med Virol"},{"issue":"1","key":"pone.0265813.ref025","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1017\/S0950268800057526","article-title":"Measurement of diphtheria and tetanus anti toxin in blood samples collected on filter paper disks","volume":"112","author":"S. Nikoletti","year":"1994","journal-title":"Epidemiol Infect"},{"issue":"4","key":"pone.0265813.ref026","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00112-19","article-title":"Field Test and Validation of the Multiplier Measles, Mumps, Rubella, and Varicella-Zoster Multiplexed Assay System in the Democratic Republic of the Congo by Using Dried Blood Spots","volume":"4","author":"SG Higgins","year":"2019","journal-title":"mSphere"},{"issue":"2","key":"pone.0265813.ref027","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1002\/jmv.21973","article-title":"The Use of Dried Blood Spots for Assessing Antibody Response to Hepatitis A Virus After Natural Infection and Vaccination","volume":"83","author":"J G Melga\u00e7o","year":"2011","journal-title":"Med Virol"},{"issue":"2","key":"pone.0265813.ref028","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1017\/S0950268896007297","article-title":"Detection of Antibodies Against Hepatitis A in Blood Spots Dried on Filter Paper. 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