{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T23:20:09Z","timestamp":1788650409709,"version":"build-2803163510"},"reference-count":79,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T00:00:00Z","timestamp":1649116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009576","name":"Kenneth Rainin Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009576","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM099537-1A1"],"award-info":[{"award-number":["R01GM099537-1A1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000076","name":"Directorate for Biological Sciences","doi-asserted-by":"publisher","award":["1551363"],"award-info":[{"award-number":["1551363"]}],"id":[{"id":"10.13039\/100000076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute of the NIH","doi-asserted-by":"crossref","award":["R03CA195305"],"award-info":[{"award-number":["R03CA195305"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The gastrointestinal microbiota begins to be acquired at birth and continually matures through early adolescence. Despite the relevance for gut health, few studies have evaluated the impact of pathobiont colonization of neonates on the severity of colitis later in life. LF82 is an adherent invasive<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>strain associated with ileal Crohn\u2019s disease. The aim of this study was to evaluate the severity of dextran sodium sulfate (DSS)-induced colitis in mice following<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82 colonization. Gnotobiotic mice harboring the altered Schaedler flora (ASF) were used as the model. While<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82 is neither adherent nor invasive, it was been demonstrated that adult ASF mice colonized with<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82 develop more severe DSS-induced colitis compared to control ASF mice treated with DSS. Therefore, we hypothesized that<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82 colonization of neonatal ASF mice would reduce the severity of DSS-induced inflammation compared to adult ASF mice colonized with<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82. To test this hypothesis, adult ASF mice were colonized with<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82 and bred to produce offspring (LF82<jats:sub>N<\/jats:sub>) that were vertically colonized with LF82. LF82<jats:sub>N<\/jats:sub>and adult-colonized (LF82<jats:sub>A<\/jats:sub>) mice were given 2.0% DSS in drinking water for seven days to trigger colitis. More severe inflammatory lesions were observed in the LF82<jats:sub>N<\/jats:sub>+ DSS mice when compared to LF82<jats:sub>A<\/jats:sub>+ DSS mice, and were characterized as transmural in most of the LF82<jats:sub>N<\/jats:sub>+ DSS mice. Colitis was accompanied by secretion of proinflammatory cytokines (IFN\u03b3, IL-17) and specific mRNA transcripts within the colonic mucosa. Using 16S rRNA gene amplicon sequencing, LF82 colonization did not induce significant changes in the ASF community; however, minimal changes in spatial redistribution by fluorescent in situ hybridization were observed. These results suggest that the age at which mice were colonized with<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>LF82 pathobiont differentially impacted severity of subsequent colitic events.<\/jats:p>","DOI":"10.1371\/journal.pone.0266005","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T17:51:35Z","timestamp":1649181095000},"page":"e0266005","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Vertical transmission of attaching and invasive E. coli from the dam to neonatal mice predisposes to more severe colitis following exposure to a colitic insult later in life"],"prefix":"10.1371","volume":"17","author":[{"given":"Meghan","family":"Wymore Brand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra L.","family":"Proctor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse M.","family":"Hostetter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naihui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1789-8000","authenticated-orcid":true,"given":"Iddo","family":"Friedberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert E.","family":"Jergens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4534-9452","authenticated-orcid":true,"given":"Michael J.","family":"Wannemuehler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,4,5]]},"reference":[{"issue":"5728","key":"pone.0266005.ref001","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1126\/science.1110591","article-title":"Diversity of the human intestinal microbial flora","volume":"308","author":"PB Eckburg","year":"2005","journal-title":"Science"},{"issue":"4","key":"pone.0266005.ref002","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.cell.2006.02.017","article-title":"Ecological and evolutionary forces shaping microbial diversity in the human intestine","volume":"124","author":"RE Ley","year":"2006","journal-title":"Cell"},{"issue":"7","key":"pone.0266005.ref003","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/sj.embor.7400731","article-title":"The gut flora as a forgotten organ","volume":"7","author":"AM O\u2019Hara","year":"2006","journal-title":"EMBO Rep"},{"issue":"12","key":"pone.0266005.ref004","doi-asserted-by":"crossref","first-page":"6578","DOI":"10.1073\/pnas.95.12.6578","article-title":"Prokaryotes: the unseen majority","volume":"95","author":"WB Whitman","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pone.0266005.ref005","doi-asserted-by":"crossref","first-page":"1035s","DOI":"10.1093\/ajcn\/69.5.1035s","article-title":"Developmental microbial ecology of the neonatal gastrointestinal tract","volume":"69","author":"RI Mackie","year":"1999","journal-title":"Am J Clin Nutr"},{"issue":"7","key":"pone.0266005.ref006","doi-asserted-by":"crossref","first-page":"e177","DOI":"10.1371\/journal.pbio.0050177","article-title":"Development of the human infant intestinal microbiota","volume":"5","author":"C Palmer","year":"2007","journal-title":"PLoS Biol"},{"issue":"8","key":"pone.0266005.ref007","doi-asserted-by":"crossref","first-page":"e1001631","DOI":"10.1371\/journal.pbio.1001631","article-title":"Mom knows best: the universality of maternal microbial transmission","volume":"11","author":"LJ Funkhouser","year":"2013","journal-title":"PLoS Biol"},{"issue":"237","key":"pone.0266005.ref008","doi-asserted-by":"crossref","first-page":"237ra65","DOI":"10.1126\/scitranslmed.3008599","article-title":"The placenta harbors a unique microbiome","volume":"6","author":"K Aagaard","year":"2014","journal-title":"Sci Transl Med"},{"issue":"1","key":"pone.0266005.ref009","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s40168-017-0268-4","article-title":"A critical assessment of the \"sterile womb\" and \"in utero colonization\" hypotheses: implications for research on the pioneer infant microbiome.","volume":"5","author":"ME Perez-Munoz","year":"2017","journal-title":"Microbiome"},{"issue":"Suppl 1","key":"pone.0266005.ref010","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/ijpo.12217","article-title":"The prenatal gut microbiome: are we colonized with bacteria in utero?","volume":"12","author":"RW Walker","year":"2017","journal-title":"Pediatr Obes."},{"issue":"4","key":"pone.0266005.ref011","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/s41591-020-0761-3","article-title":"Viable bacterial colonization is highly limited in the human intestine in utero","volume":"26","author":"E Rackaityte","year":"2020","journal-title":"Nat Med"},{"issue":"6558","key":"pone.0266005.ref012","article-title":"Prenatal maternal infection promotes tissue-specific immunity and inflammation in offspring","volume":"373","author":"AI Lim","year":"2021","journal-title":"Science"},{"issue":"6279","key":"pone.0266005.ref013","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1126\/science.aad2571","article-title":"The maternal microbiota drives early postnatal innate immune development","volume":"351","author":"M Gomez de Aguero","year":"2016","journal-title":"Science"},{"key":"pone.0266005.ref014","first-page":"471","article-title":"The response of the lymphatic tissue to the microbial flora. Studies on germfree mice","volume":"42","author":"H Bauer","year":"1963","journal-title":"Am J Pathol"},{"issue":"6","key":"pone.0266005.ref015","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1084\/jem.123.6.1013","article-title":"The enhancing effect of the microbial flora on macrophage function and the immune response. A study in germfree mice","volume":"123","author":"H Bauer","year":"1966","journal-title":"J Exp Med"},{"issue":"5","key":"pone.0266005.ref016","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.immuni.2011.03.021","article-title":"Intestinal bacterial colonization induces mutualistic regulatory T cell responses","volume":"34","author":"MB Geuking","year":"2011","journal-title":"Immunity"},{"issue":"3","key":"pone.0266005.ref017","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1136\/gut.12.3.230","article-title":"Gastrointestinal structure and function in germ-free or gnotobiotic animals","volume":"12","author":"GR Thompson","year":"1971","journal-title":"Gut"},{"key":"pone.0266005.ref018","first-page":"747","article-title":"The response of the lymphatic tissue to bacterial antigen. Studies in germfree mice","volume":"44","author":"RE Horowitz","year":"1964","journal-title":"Am J Pathol"},{"issue":"2","key":"pone.0266005.ref019","doi-asserted-by":"crossref","first-page":"118","DOI":"10.4161\/gmic.23362","article-title":"The gut microbiota and mucosal homeostasis: colonized at birth or at adulthood, does it matter?","volume":"4","author":"S El Aidy","year":"2013","journal-title":"Gut Microbes."},{"key":"pone.0266005.ref020","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1186\/1471-2164-13-335","article-title":"A microarray analysis of gnotobiotic mice indicating that microbial exposure during the neonatal period plays an essential role in immune system development","volume":"13","author":"M Yamamoto","year":"2012","journal-title":"BMC Genomics"},{"issue":"1","key":"pone.0266005.ref021","first-page":"5","article-title":"Biological Freudianism: lasting effects of early environmental influences. 1966","volume":"34","author":"R Dubos","year":"2005","journal-title":"Int J Epidemiol"},{"key":"pone.0266005.ref022","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1084\/jem.111.3.407","article-title":"The effect of the intestinal flora on the growth rate of mice, and on their susceptibility to experimental infections","volume":"111","author":"RJ Dubos","year":"1960","journal-title":"J Exp Med"},{"issue":"2","key":"pone.0266005.ref023","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.jaci.2011.10.025","article-title":"Low diversity of the gut microbiota in infants with atopic eczema","volume":"129","author":"TR Abrahamsson","year":"2012","journal-title":"J Allergy Clin Immunol"},{"issue":"6","key":"pone.0266005.ref024","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1111\/cea.12253","article-title":"Low gut microbiota diversity in early infancy precedes asthma at school age","volume":"44","author":"TR Abrahamsson","year":"2014","journal-title":"Clin Exp Allergy"},{"issue":"3","key":"pone.0266005.ref025","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.jaci.2011.04.060","article-title":"Reduced diversity of the intestinal microbiota during infancy is associated with increased risk of allergic disease at school age","volume":"128","author":"H Bisgaard","year":"2011","journal-title":"J Allergy Clin Immunol"},{"issue":"9262","key":"pone.0266005.ref026","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/S0140-6736(00)04259-8","article-title":"Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial","volume":"357","author":"M Kalliomaki","year":"2001","journal-title":"Lancet"},{"issue":"11","key":"pone.0266005.ref027","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1111\/j.1398-9995.2007.01462.x","article-title":"The role of the intestinal microbiota in the development of atopic disorders","volume":"62","author":"J Penders","year":"2007","journal-title":"Allergy"},{"issue":"5","key":"pone.0266005.ref028","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1038\/embor.2012.32","article-title":"Early life antibiotic-driven changes in microbiota enhance susceptibility to allergic asthma","volume":"13","author":"SL Russell","year":"2012","journal-title":"EMBO Rep"},{"issue":"5","key":"pone.0266005.ref029","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1034\/j.1399-3038.2002.01066.x","article-title":"Clostridium difficile, atopy and wheeze during the first year of life","volume":"13","author":"A Woodcock","year":"2002","journal-title":"Pediatr Allergy Immunol"},{"issue":"4","key":"pone.0266005.ref030","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.cell.2014.05.052","article-title":"Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences","volume":"158","author":"LM Cox","year":"2014","journal-title":"Cell"},{"issue":"3","key":"pone.0266005.ref031","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1542\/peds.2008-1146","article-title":"Antibiotic use in children is associated with increased risk of asthma","volume":"123","author":"F Marra","year":"2009","journal-title":"Pediatrics"},{"issue":"12","key":"pone.0266005.ref032","doi-asserted-by":"crossref","first-page":"2687","DOI":"10.1038\/ajg.2010.398","article-title":"Association between the use of antibiotics in the first year of life and pediatric inflammatory bowel disease","volume":"105","author":"SY Shaw","year":"2010","journal-title":"Am J Gastroenterol"},{"issue":"3","key":"pone.0266005.ref033","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1093\/ajcn\/87.3.534","article-title":"Early differences in fecal microbiota composition in children may predict overweight","volume":"87","author":"M Kalliomaki","year":"2008","journal-title":"Am J Clin Nutr"},{"issue":"1","key":"pone.0266005.ref034","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/19490976.2021.1897210","article-title":"Maternal exposures and the infant gut microbiome: a systematic review with meta-analysis","volume":"13","author":"A Grech","year":"2021","journal-title":"Gut Microbes"},{"issue":"7","key":"pone.0266005.ref035","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1038\/ncpgasthep0528","article-title":"Mechanisms of disease: pathogenesis of Crohn\u2019s disease and ulcerative colitis.","volume":"3","author":"RB Sartor","year":"2006","journal-title":"Nat Clin Pract Gastroenterol Hepatol"},{"issue":"9","key":"pone.0266005.ref036","doi-asserted-by":"crossref","first-page":"4499","DOI":"10.1128\/IAI.67.9.4499-4509.1999","article-title":"Invasive ability of an Escherichia coli strain isolated from the ileal mucosa of a patient with Crohn\u2019s disease","volume":"67","author":"J Boudeau","year":"1999","journal-title":"Infect Immun"},{"issue":"6","key":"pone.0266005.ref037","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/S0016-5085(98)70019-8","article-title":"Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn\u2019s disease","volume":"115","author":"A Darfeuille-Michaud","year":"1998","journal-title":"Gastroenterology"},{"issue":"6","key":"pone.0266005.ref038","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1002\/ibd.20860","article-title":"Molecular diversity of Escherichia coli in the human gut: new ecological evidence supporting the role of adherent-invasive E. coli (AIEC) in Crohn\u2019s disease.","volume":"15","author":"M Martinez-Medina","year":"2009","journal-title":"Inflamm Bowel Dis."},{"issue":"1","key":"pone.0266005.ref039","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1053\/j.gastro.2011.10.001","article-title":"Increasing incidence and prevalence of the inflammatory bowel diseases with time, based on systematic review","volume":"142","author":"NA Molodecky","year":"2012","journal-title":"Gastroenterology"},{"issue":"12","key":"pone.0266005.ref040","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1016\/j.cgh.2007.07.012","article-title":"The prevalence and geographic distribution of Crohn\u2019s disease and ulcerative colitis in the United States","volume":"5","author":"MD Kappelman","year":"2007","journal-title":"Clin Gastroenterol Hepatol"},{"issue":"25","key":"pone.0266005.ref041","doi-asserted-by":"crossref","first-page":"2741","DOI":"10.3748\/wjg.v24.i25.2741","article-title":"Current global trends in the incidence of pediatric-onset inflammatory bowel disease","volume":"24","author":"J Sykora","year":"2018","journal-title":"World J Gastroenterol"},{"issue":"6","key":"pone.0266005.ref042","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1172\/JCI30504","article-title":"CEACAM6 acts as a receptor for adherent-invasive E. coli, supporting ileal mucosa colonization in Crohn disease","volume":"117","author":"N Barnich","year":"2007","journal-title":"J Clin Invest"},{"issue":"10","key":"pone.0266005.ref043","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1084\/jem.20090741","article-title":"Crohn\u2019s disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM","volume":"206","author":"FA Carvalho","year":"2009","journal-title":"J Exp Med"},{"issue":"10","key":"pone.0266005.ref044","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1002\/ibd.22908","article-title":"AIEC colonization and pathogenicity: influence of previous antibiotic treatment and preexisting inflammation","volume":"18","author":"M Drouet","year":"2012","journal-title":"Inflamm Bowel Dis"},{"key":"pone.0266005.ref045","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1038\/ncomms2957","article-title":"Persistent infection with Crohn\u2019s disease-associated adherent-invasive Escherichia coli leads to chronic inflammation and intestinal fibrosis","volume":"4","author":"CL Small","year":"2013","journal-title":"Nat Commun"},{"issue":"11","key":"pone.0266005.ref046","doi-asserted-by":"crossref","first-page":"3293","DOI":"10.1007\/s10620-015-3726-5","article-title":"Attenuation of colitis by serum-derived bovine immunoglobulin\/protein isolate in a defined microbiota mouse model","volume":"60","author":"AL Henderson","year":"2015","journal-title":"Dig Dis Sci"},{"issue":"2","key":"pone.0266005.ref047","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/ilar\/ilv012","article-title":"The altered Schaedler flora: continued applications of a defined murine microbial community.","volume":"56","author":"M Wymore Brand","year":"2015","journal-title":"ILAR J"},{"issue":"4","key":"pone.0266005.ref048","doi-asserted-by":"crossref","first-page":"e0195310","DOI":"10.1371\/journal.pone.0195310","article-title":"Mice harboring pathobiont-free microbiota do not develop intestinal inflammation that normally results from an innate immune deficiency","volume":"13","author":"B Chassaing","year":"2018","journal-title":"PLoS One"},{"issue":"2","key":"pone.0266005.ref049","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.00287-14","article-title":"Draft genome sequences of the altered schaedler flora, a defined bacterial community from gnotobiotic mice.","volume":"2","author":"MJ Wannemuehler","year":"2014","journal-title":"Genome Announc."},{"issue":"8","key":"pone.0266005.ref050","doi-asserted-by":"crossref","first-page":"4778","DOI":"10.1128\/IAI.00067-06","article-title":"Adherent and invasive Escherichia coli is associated with granulomatous colitis in boxer dogs","volume":"74","author":"KW Simpson","year":"2006","journal-title":"Infect Immun"},{"issue":"5","key":"pone.0266005.ref051","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data.","volume":"7","author":"JG Caporaso","year":"2010","journal-title":"Nat Methods"},{"issue":"19","key":"pone.0266005.ref052","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"RC Edgar","year":"2010","journal-title":"Bioinformatics"},{"issue":"4","key":"pone.0266005.ref053","doi-asserted-by":"crossref","first-page":"e61217","DOI":"10.1371\/journal.pone.0061217","article-title":"phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data","volume":"8","author":"PJ McMurdie","year":"2013","journal-title":"PLoS One"},{"key":"pone.0266005.ref054","volume-title":"A language and environment for statistical computing","author":"R Core Team","year":"2013"},{"issue":"11","key":"pone.0266005.ref055","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1097\/MIB.0000000000000944","article-title":"Helicobacter bilis infection alters mucosal bacteria and modulates colitis development in defined microbiota mice","volume":"22","author":"T Atherly","year":"2016","journal-title":"Inflamm Bowel Dis"},{"issue":"7","key":"pone.0266005.ref056","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1136\/gut.2006.115428","article-title":"Helicobacter bilis: bacterial provocateur orchestrates host immune responses to commensal flora in a model of inflammatory bowel disease","volume":"56","author":"JG Fox","year":"2007","journal-title":"Gut"},{"issue":"6","key":"pone.0266005.ref057","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1016\/j.micinf.2006.01.019","article-title":"Induction of differential immune reactivity to members of the flora of gnotobiotic mice following colonization with Helicobacter bilis or Brachyspira hyodysenteriae","volume":"8","author":"AE Jergens","year":"2006","journal-title":"Microbes Infect"},{"issue":"7","key":"pone.0266005.ref058","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1136\/gut.2006.099242","article-title":"Helicobacter bilis triggers persistent immune reactivity to antigens derived from the commensal bacteria in gnotobiotic C3H\/HeN mice.","volume":"56","author":"AE Jergens","year":"2007","journal-title":"Gut"},{"issue":"10","key":"pone.0266005.ref059","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1007\/s10620-011-1701-3","article-title":"Helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger","volume":"56","author":"Z Liu","year":"2011","journal-title":"Dig Dis Sci"},{"issue":"3","key":"pone.0266005.ref060","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1172\/JCI46333","article-title":"Adherent-invasive E. coli in Crohn disease: bacterial \"agent provocateur\".","volume":"121","author":"W. Strober","year":"2011","journal-title":"J Clin Invest"},{"issue":"7","key":"pone.0266005.ref061","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1136\/gutjnl-2013-304909","article-title":"AIEC pathobiont instigates chronic colitis in susceptible hosts by altering microbiota composition","volume":"63","author":"B Chassaing","year":"2014","journal-title":"Gut"},{"issue":"8","key":"pone.0266005.ref062","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1002\/ibd.20423","article-title":"Crohn\u2019s disease-associated Escherichia coli LF82 aggravates colitis in injured mouse colon via signaling by flagellin.","volume":"14","author":"FA Carvalho","year":"2008","journal-title":"Inflamm Bowel Dis"},{"issue":"3","key":"pone.0266005.ref063","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.chom.2010.08.004","article-title":"Enterobacteriaceae act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis","volume":"8","author":"WS Garrett","year":"2010","journal-title":"Cell Host Microbe"},{"issue":"4","key":"pone.0266005.ref064","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.immuni.2015.03.004","article-title":"Distinct Commensals Induce Interleukin-1beta via NLRP3 Inflammasome in Inflammatory Monocytes to Promote Intestinal Inflammation in Response to Injury","volume":"42","author":"SU Seo","year":"2015","journal-title":"Immunity"},{"issue":"4","key":"pone.0266005.ref065","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.celrep.2017.03.080","article-title":"NLRP6 protects IL10(-\/-) mice from colitis by limiting colonization of Akkermansia muciniphila.","volume":"19","author":"SS Seregin","year":"2017","journal-title":"Cell Rep"},{"issue":"1","key":"pone.0266005.ref066","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1186\/s40168-021-01178-8","article-title":"Microbiota in mesenteric adipose tissue from Crohn\u2019s disease promote colitis in mice.","volume":"9","author":"Z He","year":"2021","journal-title":"Microbiome"},{"key":"pone.0266005.ref067","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1136\/gut.5.6.493","article-title":"Crohn\u2019s disease of the large intestine","volume":"5","author":"HE Lockhart-Mummery","year":"1964","journal-title":"Gut"},{"key":"pone.0266005.ref068","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1136\/gut.5.6.510","article-title":"Histology of Crohn\u2019s syndrome","volume":"5","author":"WJ Williams","year":"1964","journal-title":"Gut"},{"issue":"2","key":"pone.0266005.ref069","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.chom.2012.07.004","article-title":"Transient inability to manage proteobacteria promotes chronic gut inflammation in TLR5-deficient mice.","volume":"12","author":"FA Carvalho","year":"2012","journal-title":"Cell Host Microbe."},{"issue":"11","key":"pone.0266005.ref070","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.035063","article-title":"Pathogenic and non-pathogenic Escherichia coli colonization and host inflammatory response in a defined microbiota mouse model.","volume":"11","author":"ZR Stromberg","year":"2018","journal-title":"Dis Model Mech."},{"issue":"1","key":"pone.0266005.ref071","article-title":"Mouse genetic background affects transfer of an antibiotic resistance plasmid in the gastrointestinal tract","volume":"5","author":"LC Ott","year":"2020","journal-title":"mSphere"},{"issue":"20","key":"pone.0266005.ref072","article-title":"Campylobacter jejuni persistently colonizes gnotobiotic altered Schaedler flora C3H\/HeN mice and induces mild colitis","volume":"367","author":"M Wymore Brand","year":"2020","journal-title":"FEMS Microbiol Lett"},{"issue":"1","key":"pone.0266005.ref073","doi-asserted-by":"crossref","first-page":"12301","DOI":"10.1038\/s41598-018-30055-y","article-title":"AIEC infection triggers modification of gut microbiota composition in genetically predisposed mice, contributing to intestinal inflammation.","volume":"8","author":"A Bretin","year":"2018","journal-title":"Sci Rep."},{"issue":"5","key":"pone.0266005.ref074","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nri3661","article-title":"Cytokines in inflammatory bowel disease","volume":"14","author":"MF Neurath","year":"2014","journal-title":"Nat Rev Immunol"},{"issue":"4","key":"pone.0266005.ref075","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.immuni.2019.03.017","article-title":"Cytokine networks in the pathophysiology of inflammatory bowel disease","volume":"50","author":"M Friedrich","year":"2019","journal-title":"Immunity"},{"key":"pone.0266005.ref076","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.molimm.2017.11.020","article-title":"IL-17 is a protection effector against the adherent-invasive Escherichia coli in murine colitis","volume":"93","author":"HJ Zhang","year":"2018","journal-title":"Mol Immunol"},{"issue":"10","key":"pone.0266005.ref077","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1002\/ibd.21248","article-title":"IL-23\/IL-17 axis in IBD.","volume":"16","author":"M Sarra","year":"2010","journal-title":"Inflamm Bowel Dis"},{"issue":"1","key":"pone.0266005.ref078","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jcmgh.2018.02.014","article-title":"Neonatal colonic inflammation epigenetically aggravates epithelial inflammatory responses to injury in adult life","volume":"6","author":"XS Zhong","year":"2018","journal-title":"Cell Mol Gastroenterol Hepatol"},{"issue":"1","key":"pone.0266005.ref079","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1038\/s41467-020-15342-5","article-title":"Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease","volume":"11","author":"FJ Ryan","year":"2020","journal-title":"Nat Commun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0266005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T17:00:48Z","timestamp":1726938048000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0266005"}},"subtitle":[],"editor":[{"given":"Hiroyasu","family":"Nakano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,4,5]]},"references-count":79,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0266005","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,5]]}}}