{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T20:42:48Z","timestamp":1767904968711,"version":"3.49.0"},"reference-count":77,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"The Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No:31630011"],"award-info":[{"award-number":["No:31630011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Natural Science Foundation of Guangdong Province","award":["2017A03031625"],"award-info":[{"award-number":["2017A03031625"]}]},{"name":"The Science and Technology Planning Project of Guangzhou","award":["(No. 201804020084"],"award-info":[{"award-number":["(No. 201804020084"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The susceptibility of Asian tiger mosquitoes to DENV-2 in different seasons was observed in simulated field environments as a reference to design dengue fever control strategies in Guangzhou. The life table experiments of mosquitoes in four seasons were carried out in the field. The susceptibility of <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> to dengue virus was observed in both environments in Guangzhou in summer and winter. <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> was infected with dengue virus by oral feeding. On day 7 and 14 after infection, the viral load in the head, ovary, and midgut of the mosquito was detected using real-time fluorescent quantitative PCR. Immune-associated gene expression in infected mosquitoes was performed using quantitative real-time reverse transcriptase PCR. The hatching rate and pupation rate of <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> larvae in different seasons differed significantly. The winter hatching rate of larvae was lower than that in summer, and the incubation time was longer than in summer. In the winter field environment, <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> still underwent basic growth and development processes. Mosquitoes in the simulated field environment were more susceptible to DENV-2 than those in the simulated laboratory environment. In the midgut, viral RNA levels on day 7 in summer were higher than those on day 7 in winter (F = 14.459, P = 0.01); ovarian viral RNA levels on day 7 in summer were higher than those on day 7 in winter (F = 8.656, P &lt; 0.001), but there was no significant difference in the viral load at other time points (P &gt; 0.05). Dicer-2 mRNA expression on day 7 in winter was 4.071 times than that on day 7 in summer: the viral load and Dicer-2 expression correlated moderately. <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> could still develop and transmit dengue virus in winter in Guangzhou. Mosquitoes under simulated field conditions were more susceptible to DENV-2 than those under simulated laboratory conditions.<\/jats:p>","DOI":"10.1371\/journal.pone.0266128","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T17:45:14Z","timestamp":1648835114000},"page":"e0266128","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Guangzhou seasonal climate change on the development of Aedes albopictus and its susceptibility to 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in a changing climate","volume":"151","author":"KL Ebi","year":"2016","journal-title":"Environ Res"},{"issue":"7446","key":"pone.0266128.ref002","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"key":"pone.0266128.ref003","volume-title":"Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control","year":"2009"},{"issue":"5","key":"pone.0266128.ref004","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1097\/QCO.0000000000000394","article-title":"Dengue vaccines: implications for dengue control","volume":"30","author":"ML Robinson","year":"2017","journal-title":"Curr Opin Infect Dis"},{"issue":"6","key":"pone.0266128.ref005","doi-asserted-by":"crossref","first-page":"e156548","DOI":"10.1371\/journal.pone.0156548","article-title":"Epidemiological and virological characterizations of the 2014 dengue Ooutbreak in Guangzhou, China","volume":"11","author":"H Zhao","year":"2016","journal-title":"PLoS One"},{"key":"pone.0266128.ref006","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s40249-017-0352-9","article-title":"The dengue preface to endemic in mainland China: the historical largest outbreak by Aedes albopictus in Guangzhou, 2014","volume":"6","author":"L Luo","year":"2017","journal-title":"Infectious Diseases of Poverty"},{"key":"pone.0266128.ref007","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/s12916-015-0336-1","article-title":"The changing epidemiology of dengue in China,1990\u2013201 4: a descriptive analysis of 25 years of nationwide surveillance data","volume":"13","author":"SJ Lai","year":"2015","journal-title":"BMC Med"},{"key":"pone.0266128.ref008","volume-title":"Effects of seasonal climatic variation on the biological characteristics of Aedes albopictus in Guangzhou","author":"DZ Yang","year":"2019"},{"key":"pone.0266128.ref009","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1472-6785-14-3","article-title":"A meta-analysis of the factors influencing development rate variation in Aedes aegypti (Diptera: Culicidae)","volume":"14","author":"J Couret","year":"2014","journal-title":"BMC Ecol"},{"key":"pone.0266128.ref010","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1186\/1756-3305-6-351","article-title":"Modelling adult Aedes aegypti and Aedes albopictus survival at different temperatures in laboratory and field settings","volume":"6","author":"OJ Brady","year":"2013","journal-title":"Parasites Vectors"},{"issue":"11","key":"pone.0266128.ref011","doi-asserted-by":"crossref","first-page":"e50972","DOI":"10.1371\/journal.pone.0050972","article-title":"The incubation periods of dengue viruses","volume":"7","author":"M Chan","year":"2012","journal-title":"PLoS One"},{"issue":"4","key":"pone.0266128.ref012","doi-asserted-by":"crossref","first-page":"158","DOI":"10.3390\/insects9040158","article-title":"Effects of the Environmental Temperature on Aedes aegypti and Aedes albopictus Mosquitoes: A Review","volume":"9","author":"JM Reinhold","year":"2018","journal-title":"Insects"},{"issue":"3","key":"pone.0266128.ref013","doi-asserted-by":"crossref","first-page":"e0229829","DOI":"10.1371\/journal.pone.0229829","article-title":"Semi-field life-table studies of Aedes albopictus (Diptera: Culicidae) in Guangzhou, China","volume":"15","author":"DZ Yang","year":"2020","journal-title":"PLoS One"},{"issue":"2","key":"pone.0266128.ref014","first-page":"8","article-title":"The Ecological Study on the Mosquito Species of Dengue Fever in Fuzhou","author":"NX Zheng","year":"2003","journal-title":"Strait Journal of preventive medicine"},{"key":"pone.0266128.ref015","first-page":"41","article-title":"Some problems in the production and analysis of insect life table","author":"SS Liu","year":"1986","journal-title":"Insect knowledge"},{"key":"pone.0266128.ref016","first-page":"1","volume-title":"Mosquito Ecology: Field Sampling Methods","author":"M. Service","year":"1993","edition":"2"},{"key":"pone.0266128.ref017","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/jmedent\/16.3.207","article-title":"Horizontal and vertical estimates of immature survivorship for Culex tritaeniorhynchus (Diptera: Culicidae) in Pakistan","volume":"16","author":"WK Reisen","year":"1979","journal-title":"J Med Entomol"},{"key":"pone.0266128.ref018","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/jmedent\/17.3.211","article-title":"Horizontal life table characteristics of the malaria vectors Anopheles culicifacies and Anopheles stephensi (Diptera: Culicidae)","volume":"17","author":"WK Reisen","year":"1980","journal-title":"J Med Entomol"},{"key":"pone.0266128.ref019","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1093\/jmedent\/19.4.413","article-title":"Anopheles culicifacies (Diptera: Culicidae): horizontal and vertical estimates of immature development and survivorship in rural Punjab Province, Pakistan","volume":"19","author":"WK Reisen","year":"1982","journal-title":"J Med Entomol"},{"issue":"3","key":"pone.0266128.ref020","first-page":"205","article-title":"The survival times of adult Aedes albopictus under different temperatures","volume":"28","author":"HL Duan","year":"2017","journal-title":"Chin J VectorBiol & Control"},{"issue":"1","key":"pone.0266128.ref021","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1603\/0022-2585-37.1.40","article-title":"Development and survival of immature Aedes albopictus and Aedes triseriatus (Diptera: Culicidae) in the laboratory: effects of density, food, and competition on response to temperature","volume":"37","author":"HJ Teng","year":"2000","journal-title":"J Med Entomol"},{"issue":"2","key":"pone.0266128.ref022","first-page":"209","article-title":"Effect of different temperatures on the development of Aedes albopictus.","volume":"27","author":"Y Xia","year":"2016","journal-title":"Chin J Vector Biol & Control"},{"key":"pone.0266128.ref023","first-page":"35","article-title":"Life table of experimental population of Aedes albopictus at different temperatures","author":"ZL Zhong","year":"1988","journal-title":"Journal of Sun Yat Sen Medical University"},{"issue":"3","key":"pone.0266128.ref024","first-page":"193","article-title":"The larva distribution characteristics of Aedes albopictus population in Guangzhou","volume":"18","author":"ZQ Yan","year":"2007","journal-title":"Chin J Vector Biol & Control"},{"key":"pone.0266128.ref025","first-page":"379","volume":"33","author":"C Black IV W","year":"2002","journal-title":"Flavivirus susceptibility in Aedes aegypti Archives of Medical Research"},{"issue":"1","key":"pone.0266128.ref026","first-page":"47","article-title":"Survey on the susceptibility of Aedes aegypti to important flaviviruses","volume":"10","author":"XL Zheng","year":"2010","journal-title":"Acta Parasit Med Entom"},{"issue":"7","key":"pone.0266128.ref027","doi-asserted-by":"crossref","first-page":"3741","DOI":"10.3390\/v7072795","article-title":"Tissue barriers to arbovirus Infection in mosquitoes","volume":"7","author":"AW Franz","year":"2015","journal-title":"Viruses"},{"key":"pone.0266128.ref028","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1146\/annurev.en.28.010183.001305","article-title":"Intrinsic factors affecting vector competence of mosquitoes for arboviruses","volume":"28","author":"JL Hardy","year":"1983","journal-title":"Annu Rev Entomol"},{"issue":"6","key":"pone.0266128.ref029","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.4269\/ajtmh.1979.28.1045","article-title":"Variation in susceptibility to oral infection with dengue viruses among geographic strains of Aedes aegypti","volume":"28","author":"DJ Gubler","year":"1979","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"pone.0266128.ref030","doi-asserted-by":"crossref","first-page":"143","DOI":"10.4269\/ajtmh.1987.36.143","article-title":"Effect of temperature on the vector efficiency of Aedes aegypti for dengue 2 virus","volume":"36","author":"DM Watts","year":"1987","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"pone.0266128.ref031","doi-asserted-by":"crossref","first-page":"e0126703","DOI":"10.1371\/journal.pone.0126703","article-title":"Effect of larval competition on extrinsic incubation period and vectorial capacity of Aedes albopictus for dengue Virus","volume":"10","author":"J Bara","year":"2015","journal-title":"PLoS One"},{"key":"pone.0266128.ref032","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1146\/annurev.immunol.25.022106.141615","article-title":"The host defense of Drosophila melanogaster","volume":"25","author":"B Lemaitre","year":"2007","journal-title":"Annu Rev Immunol"},{"key":"pone.0266128.ref033","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1365-2583.2008.00834.x","article-title":"Semliki Forest virus strongly reduces mosquito host defence signaling","volume":"17","author":"R Fragkoudis","year":"2008","journal-title":"Insect Mol Biol"},{"key":"pone.0266128.ref034","doi-asserted-by":"crossref","first-page":"e1000098","DOI":"10.1371\/journal.ppat.1000098","article-title":"The Aedes aegypti toll pathway controls dengue virus infection","volume":"4","author":"Z Xi","year":"2008","journal-title":"PLoS Pathog"},{"key":"pone.0266128.ref035","doi-asserted-by":"crossref","first-page":"18915","DOI":"10.1073\/pnas.1205231109","article-title":"Secreted Vago restricts West Nile virus infection in Culex mosquito cells by activating the Jak-STAT pathway","volume":"109","author":"PN Paradkar","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"pone.0266128.ref036","doi-asserted-by":"crossref","first-page":"12410","DOI":"10.1038\/ncomms12410","article-title":"Virus-derived DNA drives mosquito vector tolerance to arboviral infection","volume":"7","author":"B Goic","year":"2016","journal-title":"Nat Commun"},{"key":"pone.0266128.ref037","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.3389\/fmicb.2017.02050","article-title":"Aedes aegypti molecular responses to Zika virus: Modulation of infection by the Toll and Jak\/Stat immune pathways and virus host factors","volume":"8","author":"YI Angler\u00f3-Rodr\u00edguez","year":"2017","journal-title":"Front Microbiol"},{"key":"pone.0266128.ref038","doi-asserted-by":"crossref","first-page":"649975","DOI":"10.3389\/fcimb.2021.649975","article-title":"Vector Competence for DENV-2 Among Aedes albopictus (Diptera: Culicidae) Populations in China","volume":"11","author":"Y Wei","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0266128.ref039","doi-asserted-by":"crossref","first-page":"13568","DOI":"10.1073\/pnas.0502815102","article-title":"Transgenic alteration of Toll immune pathway in the female mosquito Aedes aegypti","volume":"102","author":"G Bian","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"pone.0266128.ref040","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ibmb.2009.01.007","article-title":"The role of NF-kappaB factor REL2 in the Aedes aegypti immune response","volume":"39","author":"Y Antonova","year":"2009","journal-title":"Insect Biochem Mol Biol"},{"key":"pone.0266128.ref041","first-page":"20122437","article-title":"Specificity of resistance to dengue virus isolates is associated with genotypes of the mosquito antiviral gene Dicer-2","volume":"280","author":"L Lambrechts","year":"2013","journal-title":"Proc. Biol. Sci"},{"key":"pone.0266128.ref042","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3390\/insects9030095","article-title":"Mosquito innate immunity","volume":"9","author":"A Kumar","year":"2018","journal-title":"Insects"},{"key":"pone.0266128.ref043","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3390\/insects11020095","article-title":"Aedes aegypti (Diptera: Culicidae) immune responses with different feeding regimes following infection by the entomopathogenic fungus Metarhizium anisopliae","volume":"11","author":"S Cabral","year":"2020","journal-title":"Insects"},{"issue":"2","key":"pone.0266128.ref044","doi-asserted-by":"crossref","first-page":"e1000299","DOI":"10.1371\/journal.ppat.1000299","article-title":"Dengue Virus Type 2 Infections of Aedes aegypti Are Modulated by the Mosquito\u2019s RNA Interference Pathway","volume":"5","author":"I S\u00e1nchez-Vargas","year":"2009","journal-title":"PLoS Pathog"},{"issue":"7","key":"pone.0266128.ref045","doi-asserted-by":"crossref","first-page":"e2295","DOI":"10.1371\/journal.pntd.0002295","article-title":"Transcriptomic profiling of diverse Aedes aegypti strains reveals increased basal-level immune activation in dengue virusrefractory populations and identifies novel virus-vectormolecular interactions","volume":"7","author":"SZ Sim","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"5","key":"pone.0266128.ref046","doi-asserted-by":"crossref","first-page":"e10678","DOI":"10.1371\/journal.pone.0010678","article-title":"Dengue virus inhibits immune responses in Aedes aegypti cells","volume":"5","author":"S Sim","year":"2010","journal-title":"PLoS One"},{"issue":"9","key":"pone.0266128.ref047","doi-asserted-by":"crossref","first-page":"e1002189","DOI":"10.1371\/journal.ppat.1002189","article-title":"Alterations in the Aedes aegypti transcriptome during infection with West Nile, dengue and yellow fever viruses","volume":"7","author":"TM Colpitts","year":"2011","journal-title":"PLoS Pathog"},{"issue":"11","key":"pone.0266128.ref048","doi-asserted-by":"crossref","first-page":"e50512","DOI":"10.1371\/journal.pone.0050512","article-title":"Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection","volume":"7","author":"M Bonizzoni","year":"2012","journal-title":"PLoS One"},{"key":"pone.0266128.ref049","doi-asserted-by":"crossref","first-page":"143","DOI":"10.4269\/ajtmh.1987.36.143","article-title":"Effect of temperature on the vector efficiency of Aedes aegypti for dengue 2 virus","volume":"36","author":"SZ Sim","year":"1987","journal-title":"Am J Trop Med Hygiene"},{"key":"pone.0266128.ref050","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.4269\/ajtmh.13-0321","article-title":"The Complex Relationship between Weather and Dengue Virus Transmission","volume":"89","author":"KM Campbell","year":"2013","journal-title":"Thailand. Am J Trop Med Hygiene"},{"key":"pone.0266128.ref051","doi-asserted-by":"crossref","first-page":"100344","DOI":"10.1016\/j.epidem.2019.05.003","article-title":"Temperature impacts on dengue emergence in the United States: Investigating the role of seasonality and climate change","volume":"28","author":"MA Robert","year":"2019","journal-title":"Epidemics"},{"issue":"1884","key":"pone.0266128.ref052","first-page":"20180795","article-title":"Temperature drives Zika virus transmission: evidence from empirical and mathematical models","volume":"285","author":"LR Demakovsky","year":"2018","journal-title":"Proc Biol Sci"},{"issue":"18","key":"pone.0266128.ref053","doi-asserted-by":"crossref","first-page":"7460","DOI":"10.1073\/pnas.1101377108","article-title":"Impact of daily temperature fluctuations on dengue virus transmission by Aedes aegypti","volume":"108","author":"L Lambrechts","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0266128.ref054","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1093\/jmedent\/43.2.309","article-title":"Effects of temperature on the transmission of West Nile virus by Culex tarsalis (Diptera: Culicidae)","volume":"43","author":"WK Reisen","year":"2006","journal-title":"J Med Entomol"},{"key":"pone.0266128.ref055","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1093\/jme\/tjv082","article-title":"Extrinsic incubation rate is not accelerated in recent California strains of West Nile virus in Culex tarsalis (Diptera: Culicidae)","volume":"52","author":"ME Danforth","year":"2015","journal-title":"J Med Entomol"},{"key":"pone.0266128.ref056","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.3389\/fmicb.2017.02337","article-title":"Temperature Increase Enhances Aedes albopictus Competence to Transmit Dengue Virus","volume":"8","author":"ZZ Liu","year":"2017","journal-title":"Front Microbiol"},{"key":"pone.0266128.ref057","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/jcm.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction","volume":"30","author":"RS Lanciotti","year":"1992","journal-title":"J Clin Microbiol"},{"key":"pone.0266128.ref058","doi-asserted-by":"crossref","first-page":"e58824","DOI":"10.1371\/journal.pone.0058824","article-title":"Fluctuating temperatures at critical thermal extremes: how selected life-history trait estimates change in Aedes aegypti","volume":"8","author":"LB Carrington","year":"2013","journal-title":"PLoS ONE"},{"issue":"1","key":"pone.0266128.ref059","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1603\/ME11242","article-title":"Large diurnal temperature fluctuations negatively influence Aedes aegypti (Diptera: Culicidae) life-history traits. full text links","volume":"50","author":"LB Carrington","year":"2013","journal-title":"J Med Entomol"},{"key":"pone.0266128.ref060","doi-asserted-by":"crossref","first-page":"689","DOI":"10.4269\/ajtmh.12-0488","article-title":"Reduction of Aedes aegypti vector competence for dengue virus under large temperature fluctuations","volume":"88","author":"LB Carrington","year":"2013","journal-title":"Am J Trop Med Hyg"},{"key":"pone.0266128.ref061","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1093\/jmedent\/27.4.607","article-title":"Diel changes in adult mosquito microhabitat temperatures and their relationship to the extrinsic incubation of arboviruses in mosquitoes in Kern County, California","volume":"27","author":"RP Meyer","year":"1990","journal-title":"J Med Entomol"},{"key":"pone.0266128.ref062","article-title":"Influence of climate on malaria transmission depends on daily temperature variation","author":"KP Paaijmans","year":"2010","journal-title":"Proc Natl Acad Sci U S 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