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There are no effective methods available for processing FFPE blocks that prevent cells from mixing with other specimens. The present study evaluated 897 sheets that could potentially prevent cell transmission but allow for the movement of various solvents used in FFPE blocks. According to the International Organization for Standardization and Japanese Industrial Standards, six requirements were established for the screening of packing sheets: 1) filter opening \u22645 \u03bcm, 2) thickness \u2264100 \u03bcm, 3) chemical resistance, 4) permeability \u22651.0 \u00d7 10<jats:sup>\u22123<\/jats:sup> cm\/s, 5) water retention rate &lt;200%, and 6) cell transit test (\u22642 cells\/10 high-power fields). Polyamide, polyethylene terephthalate, and polypropylene\/polyethylene composite sheets met all criteria. A pocket, which was designed to wrap the tissue uniformly, was made of these sheets and was found to effectively block the entry of all cell types during FFPE block processing. Using a sheet pocket, no single cell from the cell pellet could pass through the outer layer. The presence or absence of the sheet pocket did not affect hematoxylin and eosin staining. When processing FFPE blocks as a biomaterial for next-generation sequencing, the sheet pocket was effective in preventing cross-contamination. This technology will in part support the precise translation of histopathological data into genome sequencing data in general pathology laboratories.<\/jats:p>","DOI":"10.1371\/journal.pone.0266947","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T17:23:43Z","timestamp":1651685023000},"page":"e0266947","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["A sheet pocket to prevent cross-contamination of formalin-fixed paraffin-embedded block for application in next generation 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