{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:29:39Z","timestamp":1770884979970,"version":"3.50.1"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T00:00:00Z","timestamp":1649894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Non-Human Primates (NHPs) harbor <jats:italic>Cryptosporidium<\/jats:italic> genotypes that can infect humans and vice versa. NHPs <jats:italic>Chlorocebus aethiops<\/jats:italic> and <jats:italic>Colobus guereza<\/jats:italic> and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of <jats:italic>Cryptosporidium<\/jats:italic>. This cross-sectional study examined the molecular prevalence and subtypes of <jats:italic>Cryptosporidium spp<\/jats:italic>. from 185 fecal samples of <jats:italic>Chlorocebus aethiops<\/jats:italic> and <jats:italic>Colobus guereza<\/jats:italic> in rural and urban areas in Ethiopia. Samples were tested for <jats:italic>Cryptosporidium<\/jats:italic> infection using nested polymerase chain reaction (PCR), and subtypes were determined by sequencing a fragment of the 60-kDa glycoprotein gene (gp60). Of the 185 samples, fifty-one (27.56%) tested positive for <jats:italic>Cryptosporidium<\/jats:italic> infection. The species detected were <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> (n = 34), <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> (n = 12), and <jats:italic>C<\/jats:italic>. <jats:italic>cuniculus<\/jats:italic> (n = 3). Mixed infection with <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> were detected in 2 samples. Four <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> family subtypes (Ia, Ib, Id, and Ie) and one <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> family subtype (IIa) were identified. <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> IaA20 (n = 7) and <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> IIaA17G1R1 (n = 6) were the most prevalent subtypes detected. These results confirm that <jats:italic>Chlorocebus aethiops<\/jats:italic> and <jats:italic>Colobus guereza<\/jats:italic> can be infected with diverse <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> subtypes that can also potentially infect humans. Additional studies could help to understand the role of NHPs in the zoonotic transmission of <jats:italic>Cryptosporidium<\/jats:italic> in Ethiopia.<\/jats:p>","DOI":"10.1371\/journal.pone.0267103","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T17:47:51Z","timestamp":1649958471000},"page":"e0267103","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genetic diversity of Cryptosporidium spp. in non-human primates in rural and\u00a0urban areas of Ethiopia"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2958-5471","authenticated-orcid":true,"given":"Ambachew W.","family":"Hailu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham","family":"Degarege","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beyene","family":"Petros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damien","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonas Yimam","family":"Ayene","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ven-ceslas","family":"Villier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelmounaim","family":"Mouhajir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loic","family":"Favennec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romy","family":"Razakandrainibe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haileeysus","family":"Adamu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,4,14]]},"reference":[{"issue":"3","key":"pone.0267103.ref001","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.pt.2019.12.016","article-title":"A One Health Approach to Tackle Cryptosporidiosis","volume":"36","author":"EA Innes","year":"2020","journal-title":"Trends Parasitol"},{"issue":"5","key":"pone.0267103.ref002","doi-asserted-by":"crossref","first-page":"R193","DOI":"10.1016\/j.cub.2017.11.070","article-title":"Cryptosporidium.","volume":"28","author":"AR Gibson","year":"2018","journal-title":"Curr Biol"},{"issue":"9","key":"pone.0267103.ref003","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/S1473-3099(17)30276-1","article-title":"Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015","volume":"17","author":"C Troeger","year":"2017","journal-title":"Lancet Infect Dis"},{"issue":"9","key":"pone.0267103.ref004","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1093\/oxfordjournals.aje.a009015","article-title":"Cryptosporidiosis among patients infected with human immunodeficiency virus. 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