{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T12:01:59Z","timestamp":1792929719162,"version":"4.5.0"},"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01CA093900"],"award-info":[{"award-number":["P01CA093900"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21NS107879"],"award-info":[{"award-number":["R21NS107879"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32CA254147"],"award-info":[{"award-number":["F32CA254147"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017065","name":"Rogel Cancer Center","doi-asserted-by":"crossref","award":["G020989"],"award-info":[{"award-number":["G020989"]}],"id":[{"id":"10.13039\/100017065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["PC170089"],"award-info":[{"award-number":["PC170089"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["1I01BX001370"],"award-info":[{"award-number":["1I01BX001370"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Society of Neurological Surgeons"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Roughly 400,000 people in the U.S. are living with bone metastases, the vast majority occurring in the spine. Metastases to the spine result in fractures, pain, paralysis, and significant health care costs. This predilection for cancer to metastasize to the bone is seen across most cancer histologies, with the greatest incidence seen in prostate, breast, and lung cancer. The molecular process involved in this predilection for axial versus appendicular skeleton is not fully understood, although it is likely that a combination of tumor and local micro-environmental factors plays a role. Immune cells are an important constituent of the bone marrow microenvironment and many of these cells have been shown to play a significant role in tumor growth and progression in soft tissue and bone disease. With this in mind, we sought to examine the differences in immune landscape between axial and appendicular bones in the normal noncancerous setting in order to obtain an understanding of these landscapes. To accomplish this, we utilized mass cytometry by time-of-flight (CyTOF) to examine differences in the immune cell landscapes between the long bone and vertebral body bone marrow from patient clinical samples and C57BL\/6J mice. We demonstrate significant differences between immune populations in both murine and human marrow with a predominance of myeloid progenitor cells in the spine. Additionally, cytokine analysis revealed differences in concentrations favoring a more myeloid enriched population of cells in the vertebral body bone marrow. These differences could have clinical implications with respect to the distribution and permissive growth of bone metastases.<\/jats:p>","DOI":"10.1371\/journal.pone.0267642","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T17:36:17Z","timestamp":1651080977000},"page":"e0267642","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Differential immune landscapes in appendicular versus axial skeleton"],"prefix":"10.1371","volume":"17","author":[{"given":"Aqila A.","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9930-3578","authenticated-orcid":true,"given":"Michael J.","family":"Strong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6791-6537","authenticated-orcid":true,"given":"Tyler","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Rocco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey W.","family":"Siegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Clines","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany B.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan T.","family":"Keller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1116-3422","authenticated-orcid":true,"given":"Nicholas J.","family":"Szerlip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"issue":"1","key":"pone.0267642.ref001","doi-asserted-by":"crossref","first-page":"94","DOI":"10.3171\/2010.3.SPINE09202","article-title":"Diagnosis and management of metastatic spine disease","volume":"13","author":"DM Sciubba","year":"2010","journal-title":"J Neurosurg Spine"},{"issue":"4","key":"pone.0267642.ref002","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1302\/0301-620X.84B4.0840521","article-title":"The surgical treatment of bony metastases of the spine and limbs","volume":"84","author":"P Bohm","year":"2002","journal-title":"J Bone Joint Surg Br"},{"issue":"3","key":"pone.0267642.ref003","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0167-8140(00)00199-7","article-title":"The surgical treatment of metastatic disease of the spine","volume":"56","author":"NC Hatrick","year":"2000","journal-title":"Radiother Oncol"},{"issue":"7","key":"pone.0267642.ref004","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.2106\/00004623-198668070-00025","article-title":"Metastatic disease of the spine","volume":"68","author":"KD Harrington","year":"1986","journal-title":"J Bone Joint Surg Am"},{"issue":"5","key":"pone.0267642.ref005","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1053\/hp.2000.6698","article-title":"Metastatic patterns of prostate cancer: An autopsy study of 1,589 patients","volume":"31","author":"L Bubendorf","year":"2000","journal-title":"Hum Pathol"},{"key":"pone.0267642.ref006","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/978-1-4020-9819-2_2","volume-title":"In: Kardamakis DBone Metastases: A translational and clinical approach","author":"GD Roodman","year":"2009"},{"issue":"8","key":"pone.0267642.ref007","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1038\/nrc867","article-title":"Metastasis to bone: causes, consequences and therapeutic opportunities","volume":"2","author":"GR Mundy","year":"2002","journal-title":"Nat Rev Cancer"},{"issue":"6","key":"pone.0267642.ref008","doi-asserted-by":"crossref","first-page":"176","DOI":"10.3390\/cancers10060176","article-title":"Emerging and established models of bone metastasis","volume":"10","author":"AH Jinnah","year":"2018","journal-title":"Cancers"},{"issue":"4","key":"pone.0267642.ref009","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/nrendo.2010.227","article-title":"Bone metastasis: mechanisms and therapeutic opportunities","volume":"7","author":"LJ Suva","year":"2011","journal-title":"Nat Rev Endocrinol"},{"issue":"3","key":"pone.0267642.ref010","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.radonc.2015.10.015","article-title":"Incidence and treatment patterns of complicated bone metastases in a population-based radiotherapy program","volume":"118","author":"MS Tiwana","year":"2016","journal-title":"Radiother Oncol"},{"key":"pone.0267642.ref011","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/978-1-4615-3398-6_8","article-title":"Role of the vertebral venous system in metastatic spread of cancer cells to the bone","volume":"324","author":"M Harada","year":"1992","journal-title":"Adv Exp Med Biol"},{"issue":"3421","key":"pone.0267642.ref012","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/S0140-6736(00)49915-0","article-title":"The distribution of secondary growths in cancer of the breast","volume":"133","author":"S. Paget","year":"1889","journal-title":"The Lancet"},{"issue":"7667","key":"pone.0267642.ref013","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nature23306","article-title":"Integrative clinical genomics of metastatic cancer","volume":"548","author":"DR Robinson","year":"2017","journal-title":"Nature"},{"issue":"4","key":"pone.0267642.ref014","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.mric.2010.07.003","article-title":"Bone marrow","volume":"18","author":"DT Murphy","year":"2010","journal-title":"Magn Reson Imaging Clin N Am"},{"issue":"12","key":"pone.0267642.ref015","doi-asserted-by":"crossref","first-page":"448","DOI":"10.4329\/wjr.v7.i12.448","article-title":"Magnetic resonance imaging of the spinal marrow: Basic understanding of the normal marrow pattern and its variant","volume":"7","author":"MR Nouh","year":"2015","journal-title":"World J Radiol"},{"issue":"9","key":"pone.0267642.ref016","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s002560050423","article-title":"Magnetic resonance imaging of the normal bone marrow","volume":"27","author":"BC Vande Berg","year":"1998","journal-title":"Skeletal Radiol"},{"issue":"1","key":"pone.0267642.ref017","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/BF00361188","article-title":"Red-yellow marrow conversion: its effect on the location of some solitary bone lesions","volume":"14","author":"ME Kricun","year":"1985","journal-title":"Skeletal Radiol"},{"issue":"2","key":"pone.0267642.ref018","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1615\/CritRevEukarGeneExpr.v19.i2.20","article-title":"Marrow fat and the bone microenvironment: developmental, functional, and pathological implications","volume":"19","author":"CJ Rosen","year":"2009","journal-title":"Crit Rev Eukaryot Gene Expr"},{"issue":"Suppl 1","key":"pone.0267642.ref019","doi-asserted-by":"crossref","first-page":"S181","DOI":"10.1007\/s00247-012-2582-0","article-title":"Marrow: red, yellow and bad","volume":"43","author":"RP Guillerman","year":"2013","journal-title":"Pediatr Radiol"},{"issue":"4","key":"pone.0267642.ref020","first-page":"45","article-title":"Bone marrow reconversion\u2014imaging of physiological changes in bone marrow","volume":"77","author":"A Malkiewicz","year":"2012","journal-title":"Pol J Radiol"},{"issue":"2","key":"pone.0267642.ref021","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.mric.2007.03.002","article-title":"MR imaging evaluation of the bone marrow and marrow infiltrative disorders of the lumbar spine","volume":"15","author":"F Alyas","year":"2007","journal-title":"Magn Reson Imaging Clin N Am"},{"issue":"4","key":"pone.0267642.ref022","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1158\/1078-0432.CCR-14-1860","article-title":"Immune escape mechanisms as a guide for cancer immunotherapy","volume":"21","author":"GL Beatty","year":"2015","journal-title":"Clin Cancer Res"},{"issue":"4","key":"pone.0267642.ref023","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.ccell.2015.03.007","article-title":"The journey from discoveries in fundamental immunology to cancer immunotherapy","volume":"27","author":"JF Miller","year":"2015","journal-title":"Cancer Cell"},{"key":"pone.0267642.ref024","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1038\/nm.3394","article-title":"Microenvironmental regulation of tumor progression and metastasis","volume":"19","author":"DF Quail","year":"2013","journal-title":"Nat Med"},{"issue":"1","key":"pone.0267642.ref025","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/cmi.2011.47","article-title":"Bone marrow and the control of immunity","volume":"9","author":"E Zhao","year":"2012","journal-title":"Cell Mol Immunol"},{"issue":"7350","key":"pone.0267642.ref026","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/nature10160","article-title":"In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche","volume":"474","author":"J Fujisaki","year":"2011","journal-title":"Nature"},{"issue":"3","key":"pone.0267642.ref027","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.stem.2018.01.017","article-title":"CD150(high) bone marrow Tregs maintain hematopoietic stem cell quiescence and immune privilege via adenosine","volume":"22","author":"Y Hirata","year":"2018","journal-title":"Cell Stem Cell"},{"issue":"5","key":"pone.0267642.ref028","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.cell.2019.10.029","article-title":"Differences in tumor microenvironment dictate T helper lineage polarization and response to immune checkpoint therapy","volume":"179","author":"S Jiao","year":"2019","journal-title":"Cell"},{"issue":"6244","key":"pone.0267642.ref029","doi-asserted-by":"crossref","first-page":"1259425","DOI":"10.1126\/science.1259425","article-title":"Immunology. An interactive reference framework for modeling a dynamic immune system","volume":"349","author":"MH Spitzer","year":"2015","journal-title":"Science"},{"issue":"1","key":"pone.0267642.ref030","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/s41698-017-0031-0","article-title":"Immune cell profiling in cancer: molecular approaches to cell-specific identification","volume":"1","author":"YA Lyons","year":"2017","journal-title":"NPJ Precis Oncol"},{"issue":"1","key":"pone.0267642.ref031","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.immuni.2012.01.002","article-title":"Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes","volume":"36","author":"EW Newell","year":"2012","journal-title":"Immunity"},{"key":"pone.0267642.ref032","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2014.10.010","article-title":"CyTOF supports efficient detection of immune cell subsets from small samples","volume":"415","author":"Y Yao","year":"2014","journal-title":"J Immunol Methods"},{"issue":"1","key":"pone.0267642.ref033","first-page":"1","article-title":"Web-based analysis and publication of flow cytometry experiments","volume":"53","author":"N Kotecha","year":"2010","journal-title":"Curr Protoc Cytom"},{"issue":"9","key":"pone.0267642.ref034","doi-asserted-by":"crossref","first-page":"e1005112","DOI":"10.1371\/journal.pcbi.1005112","article-title":"Cytofkit: A bioconductor package for an integrated mass cytometry data analysis pipeline","volume":"12","author":"H Chen","year":"2016","journal-title":"PLoS Comput Biol"},{"issue":"1","key":"pone.0267642.ref035","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.cell.2015.05.047","article-title":"Data-driven phenotypic dissection of AML reveals progenitor-like cells that correlate with prognosis","volume":"162","author":"JH Levine","year":"2015","journal-title":"Cell"},{"issue":"Suppl 2","key":"pone.0267642.ref036","doi-asserted-by":"crossref","first-page":"e12989","DOI":"10.1111\/eci.12989","article-title":"Neutrophils as myeloid-derived suppressor cells","volume":"48","author":"CEM Aarts","year":"2018","journal-title":"Eur J Clin Invest"},{"issue":"7","key":"pone.0267642.ref037","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.it.2012.02.010","article-title":"A deep profiler\u2019s guide to cytometry","volume":"33","author":"SC Bendall","year":"2012","journal-title":"Trends Immunol"},{"issue":"148","key":"pone.0267642.ref038","article-title":"Preparation of whole bone marrow for mass cytometry analysis of neutrophil-lineage cells","author":"YP Zhu","year":"2019","journal-title":"J Vis Exp"},{"issue":"1","key":"pone.0267642.ref039","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/s41467-020-14396-9","article-title":"Sexual-dimorphism in human immune system aging","volume":"11","author":"EJ Marquez","year":"2020","journal-title":"Nat Commun"},{"issue":"6","key":"pone.0267642.ref040","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s11914-017-0411-y","article-title":"Immune contributions to osteoarthritis","volume":"15","author":"EBP Lopes","year":"2017","journal-title":"Curr Osteoporos Rep"},{"issue":"1","key":"pone.0267642.ref041","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1097\/BOR.0000000000000353","article-title":"Role of low-grade inflammation in osteoarthritis","volume":"29","author":"CR Scanzello","year":"2017","journal-title":"Curr Opin Rheumatol"},{"issue":"16","key":"pone.0267642.ref042","doi-asserted-by":"crossref","first-page":"4965","DOI":"10.1158\/0008-5472.CAN-13-0661","article-title":"Targeting the tumor microenvironment: from understanding pathways to effective clinical trials","volume":"73","author":"H Fang","year":"2013","journal-title":"Cancer Res"},{"issue":"4","key":"pone.0267642.ref043","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nrc3910","article-title":"Metastasis prevention by targeting the dormant niche","volume":"15","author":"CM Ghajar","year":"2015","journal-title":"Nat Rev Cancer"},{"issue":"1","key":"pone.0267642.ref044","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.canlet.2013.08.029","article-title":"The role played by the microenvironment in site-specific metastasis","volume":"352","author":"A Klein-Goldberg","year":"2014","journal-title":"Cancer Lett"},{"issue":"8","key":"pone.0267642.ref045","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1038\/nm.2830","article-title":"Silencing of Irf7 pathways in breast cancer cells promotes bone metastasis through immune escape","volume":"18","author":"BN Bidwell","year":"2012","journal-title":"Nat Med"},{"key":"pone.0267642.ref046","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/bonekey.2014.95","article-title":"Immune regulation of bone metastasis","volume":"3","author":"AH Capietto","year":"2014","journal-title":"Bonekey Rep"},{"issue":"20","key":"pone.0267642.ref047","doi-asserted-by":"crossref","first-page":"6185","DOI":"10.1158\/0008-5472.CAN-13-1364","article-title":"Targeting FSTL1 prevents tumor bone metastasis and consequent immune dysfunction","volume":"73","author":"C Kudo-Saito","year":"2013","journal-title":"Cancer Res"},{"key":"pone.0267642.ref048","doi-asserted-by":"crossref","first-page":"12150","DOI":"10.1038\/ncomms12150","article-title":"Recommendations for myeloid-derived suppressor cell nomenclature and characterization standards","volume":"7","author":"V Bronte","year":"2016","journal-title":"Nat Commun"},{"issue":"1","key":"pone.0267642.ref049","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1158\/0008-5472.CAN-06-3037","article-title":"The terminology issue for myeloid-derived suppressor cells","volume":"67","author":"DI Gabrilovich","year":"2007","journal-title":"Cancer Res"},{"issue":"4","key":"pone.0267642.ref050","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/nri3175","article-title":"Coordinated regulation of myeloid cells by tumours","volume":"12","author":"DI Gabrilovich","year":"2012","journal-title":"Nat Rev Immunol"},{"issue":"3","key":"pone.0267642.ref051","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.it.2016.01.004","article-title":"The nature of myeloid-derived suppressor cells in the tumor microenvironment","volume":"37","author":"V Kumar","year":"2016","journal-title":"Trends Immunol"},{"key":"pone.0267642.ref052","first-page":"95","volume-title":"Advances in Cancer Research","author":"KH Parker","year":"2015"},{"key":"pone.0267642.ref053","doi-asserted-by":"crossref","first-page":"523","DOI":"10.3389\/fimmu.2015.00523","article-title":"Paving the road to tumor development and spreading: Myeloid-derived suppressor cells are ruling the fate","volume":"6","author":"Y Meirow","year":"2015","journal-title":"Front Immunol"},{"issue":"2","key":"pone.0267642.ref054","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.coi.2010.01.021","article-title":"Myeloid-derived suppressor cell heterogeneity and subset definition","volume":"22","author":"E Peranzoni","year":"2010","journal-title":"Curr Opin Immunol"},{"issue":"3","key":"pone.0267642.ref055","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00430-010-0151-4","article-title":"Subsets, expansion and activation of myeloid-derived suppressor cells","volume":"199","author":"E Ribechini","year":"2010","journal-title":"Med Microbiol Immunol"},{"key":"pone.0267642.ref056","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.3389\/fimmu.2019.01401","article-title":"Circulating myeloid derived suppressor cells (MDSC) that accumulate in premalignancy share phenotypic and functional characteristics with MDSC in cancer","volume":"10","author":"P Ma","year":"2019","journal-title":"Front Immunol"},{"issue":"5","key":"pone.0267642.ref057","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/nrc.2017.6","article-title":"Pre-metastatic niches: organ-specific homes for metastases","volume":"17","author":"H Peinado","year":"2017","journal-title":"Nat Rev Cancer"},{"issue":"2","key":"pone.0267642.ref058","doi-asserted-by":"crossref","first-page":"e31524","DOI":"10.1371\/journal.pone.0031524","article-title":"Transcriptomic analysis comparing tumor-associated neutrophils with granulocytic myeloid-derived suppressor cells and normal neutrophils","volume":"7","author":"ZG Fridlender","year":"2012","journal-title":"PLoS One"},{"key":"pone.0267642.ref059","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.smim.2017.12.004","article-title":"Neutrophils and PMN-MDSC: Their biological role and interaction with stromal cells","volume":"35","author":"J Zhou","year":"2018","journal-title":"Semin Immunol"},{"issue":"2","key":"pone.0267642.ref060","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.immuni.2016.01.014","article-title":"CD45 phosphatase inhibits STAT3 transcription factor activity in myeloid cells and promotes tumor-associated macrophage differentiation","volume":"44","author":"V Kumar","year":"2016","journal-title":"Immunity"},{"issue":"9","key":"pone.0267642.ref061","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1007\/s00262-011-1178-0","article-title":"Pancreatic adenocarcinoma induces bone marrow mobilization of myeloid-derived suppressor cells which promote primary tumor growth","volume":"61","author":"MR Porembka","year":"2012","journal-title":"Cancer Immunol Immunother"},{"issue":"7","key":"pone.0267642.ref062","doi-asserted-by":"crossref","first-page":"4666","DOI":"10.4049\/jimmunol.181.7.4666","article-title":"Proinflammatory S100 proteins regulate the accumulation of myeloid-derived suppressor cells","volume":"181","author":"P Sinha","year":"2008","journal-title":"J Immunol"},{"issue":"11","key":"pone.0267642.ref063","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1084\/jem.20130281","article-title":"Down-regulation of PLC\u03b32-\u03b2-catenin pathway promotes activation and expansion of myeloid-derived suppressor cells in cancer","volume":"210","author":"A-H Capietto","year":"2013","journal-title":"J Exp Med"},{"key":"pone.0267642.ref064","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/143526","article-title":"The Impact of immune system in regulating bone metastasis formation by osteotropic tumors","volume":"2015","author":"L D\u2019Amico","year":"2015","journal-title":"J Immunol Res"},{"issue":"1","key":"pone.0267642.ref065","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.semcancer.2005.07.005","article-title":"Myeloid suppressor cells in cancer: recruitment, phenotype, properties, and mechanisms of immune suppression","volume":"16","author":"P Serafini","year":"2006","journal-title":"Semin Cancer Biol"},{"issue":"3","key":"pone.0267642.ref066","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s10555-006-9002-6","article-title":"Effect of tumor-derived cytokines and growth factors on differentiation and immune suppressive features of myeloid cells in cancer","volume":"25","author":"S Kusmartsev","year":"2006","journal-title":"Cancer Metastasis Rev"},{"issue":"4","key":"pone.0267642.ref067","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1007\/s00262-011-1116-1","article-title":"A Th1 cytokine-enriched microenvironment enhances tumor killing by activated T cells armed with bispecific antibodies and inhibits the development of myeloid-derived suppressor cells","volume":"61","author":"A Thakur","year":"2012","journal-title":"Cancer Immunol Immunother"},{"issue":"15","key":"pone.0267642.ref068","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1038\/onc.2016.367","article-title":"CXCL2\/MIF-CXCR2 signaling promotes the recruitment of myeloid-derived suppressor cells and is correlated with prognosis in bladder cancer","volume":"36","author":"H Zhang","year":"2017","journal-title":"Oncogene"},{"issue":"1","key":"pone.0267642.ref069","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s11060-021-03752-4","article-title":"Dura promotes metastatic potential in prostate cancer through the CXCR2 pathway","volume":"153","author":"MJ Strong","year":"2021","journal-title":"J Neurooncol"},{"issue":"Suppl 4","key":"pone.0267642.ref070","doi-asserted-by":"crossref","first-page":"S616","DOI":"10.21037\/tcr.2016.10.06","article-title":"The emerging role of chemokine receptor CXCR2 in cancer progression","volume":"5","author":"T Jaffer","year":"2016","journal-title":"Transl Cancer Res"},{"issue":"5","key":"pone.0267642.ref071","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1124\/mi.10.5.3","article-title":"Fractalkine\/CX3CL1: a potential new target for inflammatory diseases","volume":"10","author":"BA Jones","year":"2010","journal-title":"Mol Interv"},{"issue":"1\u20132","key":"pone.0267642.ref072","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jneuroim.2010.05.007","article-title":"Role of CX3CR1\/CX3CL1 axis in primary and secondary involvement of the nervous system by cancer","volume":"224","author":"F Marchesi","year":"2010","journal-title":"J Neuroimmunol"},{"issue":"4","key":"pone.0267642.ref073","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1016\/S0002-9440(10)62537-0","article-title":"Fractalkine: a novel angiogenic chemokine in rheumatoid arthritis","volume":"159","author":"MV Volin","year":"2001","journal-title":"Am J Pathol"},{"issue":"1","key":"pone.0267642.ref074","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1186\/s40425-019-0757-z","article-title":"IL-17 inhibits CXCL9\/10-mediated recruitment of CD8(+) cytotoxic T cells and regulatory T cells to colorectal tumors","volume":"7","author":"J Chen","year":"2019","journal-title":"J Immunother Cancer"},{"issue":"5","key":"pone.0267642.ref075","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.4049\/jimmunol.0902574","article-title":"IL-17 promotes tumor development through the induction of tumor promoting microenvironments at tumor sites and myeloid-derived suppressor cells","volume":"184","author":"D He","year":"2010","journal-title":"J Immunol"},{"issue":"7556","key":"pone.0267642.ref076","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nature14282","article-title":"IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis","volume":"522","author":"SB Coffelt","year":"2015","journal-title":"Nature"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0267642","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T17:37:50Z","timestamp":1651081070000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0267642"}},"subtitle":[],"editor":[{"given":"Federico","family":"Quaini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,4,27]]},"references-count":76,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0267642","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,27]]}}}