{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:27:38Z","timestamp":1791872858678,"version":"4.3.4"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T00:00:00Z","timestamp":1651104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004071","name":"Khon Kaen University","doi-asserted-by":"publisher","award":["PD2564-13"],"award-info":[{"award-number":["PD2564-13"]}],"id":[{"id":"10.13039\/501100004071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["RTA6280005"],"award-info":[{"award-number":["RTA6280005"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Identification of \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (SEA and THAI deletions) is essential in preventing and controlling of severe thalassemia diseases. We have developed the LAMP colorimetric assays for the detection of these two thalassemia defects and validated them in population screening and prenatal diagnosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Three LAMP colorimetric assays specific for \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (SEA deletion), \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (THAI deletion) and normal DNA sequence were developed. These assays were validated on 341 subjects who had initial thalassemia screening positive and various thalassemia genotypes. Prenatal diagnosis of \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (SEA deletion) was done on 33 fetuses at risk of having Hb Bart\u2019s hydrops fetalis syndrome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The LAMP colorimetric assays for \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (SEA and THAI deletions) could be clearly interpreted by naked eyes. The assay for \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (SEA deletion) showed a 100% (62\/62 x 100) sensitivity and 98.2% (274\/279 x 100) specificity whereas, that of the \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (THAI deletion) showed 100% (1\/1 x 100) sensitivity and 99.7% (339\/340 x 100) specificity. We obtained a 100% concordant prenatal diagnosis results using LAMP assays of \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassemia (SEA deletion) in 33 fetuses as compared to the conventional PCR analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The LAMP colorimetric assays developed are simple, rapid, and do not require sophisticated equipment. Inclusion of the LAMP tests in the existing screening protocol significantly reduce the screening cost and the molecular analysis workload, which should prove useful in the prevention and control program of hemoglobinopathies in the region.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0267832","type":"journal-article","created":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T16:04:27Z","timestamp":1651161867000},"page":"e0267832","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Loop-mediated isothermal amplification (LAMP) colorimetric phenol red assay for rapid identification of \u03b10-thalassemia: Application to population screening and prenatal diagnosis"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6035-3006","authenticated-orcid":true,"given":"Wittaya","family":"Jomoui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hataichanok","family":"Srivorakun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriyakorn","family":"Chansai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9142-5079","authenticated-orcid":true,"given":"Supan","family":"Fucharoen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,4,28]]},"reference":[{"key":"pone.0267832.ref001","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1750-1172-5-13","article-title":"Alpha-thalassaemia","volume":"5","author":"CL Harteveld","year":"2010","journal-title":"Orphanet J Rare Dis"},{"key":"pone.0267832.ref002","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1038\/jhg.2017.41","article-title":"Genetic origin of \u03b10-thalassemia (SEA deletion) in Southeast Asian populations and application to accurate prenatal diagnosis of Hb Bart\u2019s hydrops fetalis syndrome","volume":"62","author":"W Jomoui","year":"2017","journal-title":"J Hum Genet"},{"key":"pone.0267832.ref003","first-page":"647","article-title":"Thalassemia in Southeast Asia: problems and strategy for prevention and control","volume":"23","author":"S Fucharoen","year":"1992","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"pone.0267832.ref004","doi-asserted-by":"crossref","first-page":"47","DOI":"10.3109\/03630269.2011.637149","article-title":"Micromapping of thalassemia and hemoglobinopathies in different regions of northeast Thailand and Vientiane","volume":"36","author":"J Tritipsombut","year":"2012","journal-title":"Lao PDR. Hemoglobin"},{"key":"pone.0267832.ref005","doi-asserted-by":"crossref","first-page":"e40580","DOI":"10.7554\/eLife.40580","article-title":"Estimating the burden of \u03b1-thalassaemia in Thailand using a comprehensive prevalence database for Southeast Asia","volume":"8","author":"C Hockham","year":"2019","journal-title":"Elife"},{"key":"pone.0267832.ref006","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1196\/annals.1345.004","article-title":"Thalassemia: Hb H disease and Hb Bart\u2019s hydrops fetalis","volume":"1054","author":"DHK Chui","year":"2005","journal-title":"Ann NY Acad Sci"},{"key":"pone.0267832.ref007","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1136\/jcp.2003.014456","article-title":"The prevalence and spectrum of alpha and beta thalassaemia in Guangdong province: implications for the future health burden and population screening","volume":"57","author":"XM Xu","year":"2004","journal-title":"J Clin Pathol"},{"key":"pone.0267832.ref008","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1159\/000076523","article-title":"The diverse molecular basis and hematologic features of Hb H and AEBart\u2019s diseases in northeast Thailand","volume":"111","author":"S Boonsa","year":"2004","journal-title":"Acta Haematol"},{"key":"pone.0267832.ref009","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/ijlh.13118","article-title":"Molecular basis of Hb H and AEBart\u2019s diseases in the Lao People\u2019s Democratic Republic","volume":"42","author":"K Singha","year":"2020","journal-title":"Int J lab Hematol"},{"key":"pone.0267832.ref010","first-page":"364","article-title":"A simplified screening strategy for thalassaemia and haemoglobin E in rural communities in south-east Asia","volume":"82","author":"G Fucharoen","year":"2004","journal-title":"Bull World Health Organ"},{"key":"pone.0267832.ref011","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1309\/FUF9EVGQ24V1PKTP","article-title":"A reliable screening protocol for thalassemia and hemoglobinopathies in pregnancy: an alternative approach to electronic blood cell counting","volume":"123","author":"K Sanchaisuriya","year":"2005","journal-title":"Am J Clin Pathol"},{"key":"pone.0267832.ref012","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1159\/000355187","article-title":"A newly modified hemoglobin H inclusion test as a secondary screening for \u03b10-thalassemia in Southeast Asian populations","volume":"132","author":"G Fucharoen","year":"2014","journal-title":"Acta Haematol"},{"key":"pone.0267832.ref013","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1177\/0969141314538611","article-title":"Routine screening for \u03b1-thalassemia using an immunochromatographic strip assay for hemoglobin Bart\u2019s","volume":"21","author":"P Prayalaw","year":"2014","journal-title":"J Med Screen"},{"key":"pone.0267832.ref014","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1136\/jclinpath-2016-203765","article-title":"Screening of (-SEA) \u03b1-thalassaemia using an immunochromatographic strip assay for the \u03b6-globin chain in a population with a high prevalence and heterogeneity of haemoglobinopathies","volume":"70","author":"W Jomoui","year":"2017","journal-title":"J Clin Pathol"},{"key":"pone.0267832.ref015","first-page":"111","article-title":"Improvement of \u03b10-thalassemia screening using combined osmotic fragility, dichlorophenolindophenol and Hb H inclusion test","volume":"56","author":"A Chaibunruang","year":"2010","journal-title":"Clin Lab"},{"key":"pone.0267832.ref016","doi-asserted-by":"crossref","first-page":"12491","DOI":"10.1021\/acs.chemrev.5b00428","article-title":"Isothermal Amplification of Nucleic Acids","volume":"115","author":"Y Zhao","year":"2015","journal-title":"Chem Rev"},{"key":"pone.0267832.ref017","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3390\/bios3010018","article-title":"Isothermal amplification methods for the detection of nucleic acids in microfluidic devices","volume":"3","author":"LM Zanoli","year":"2012","journal-title":"Biosensors (Basel)"},{"key":"pone.0267832.ref018","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/jam.13647","article-title":"Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms","volume":"124","author":"YP Wong","year":"2018","journal-title":"J Appl Microbiol"},{"key":"pone.0267832.ref019","doi-asserted-by":"crossref","first-page":"465","DOI":"10.14202\/vetworld.2016.465-469","article-title":"Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases","volume":"9","author":"PR Sahoo","year":"2016","journal-title":"Vet World"},{"key":"pone.0267832.ref020","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2144\/000114253","article-title":"Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes","volume":"58","author":"NA Tanner","year":"2015","journal-title":"Biotechniques"},{"key":"pone.0267832.ref021","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/jam.14770","article-title":"WarmStart colorimetric RT-LAMP for the rapid, sensitive and specific detection of Enteroviruses A-D targeting the 5\u2019UTR region","volume":"130","author":"M Daskou","year":"2021","journal-title":"J Appl Microbiol"},{"key":"pone.0267832.ref022","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/j.1751-553X.2011.01301.x","article-title":"Presumptive diagnosis of common haemoglobinopathies in Southeast Asia using a capillary electrophoresis system","volume":"33","author":"G Fucharoen","year":"2011","journal-title":"Int J Lab Hematol"},{"key":"pone.0267832.ref023","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1182\/blood.V95.1.360","article-title":"Single-tube multiplex-PCR screen for common deletional determinants of alpha-thalassemia","volume":"95","author":"SS Chong","year":"2000","journal-title":"Blood"},{"key":"pone.0267832.ref024","first-page":"1092","article-title":"Interaction of hemoglobin E and several forms of \u03b1-thalassemia in Cambodian families","volume":"88","author":"S Fucharoen","year":"2003","journal-title":"Haematologica"},{"key":"pone.0267832.ref025","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1002\/pd.2514","article-title":"Prevention of severe thalassemia in northeast Thailand: 16 years of experience at a single university center","volume":"30","author":"S Yamsri","year":"2010","journal-title":"Prenat Diagn"},{"key":"pone.0267832.ref026","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1182\/blood.V91.7.2213","article-title":"Hydrops fetalis caused by alpha thalassemia: an emerging health care problem","volume":"91","author":"DHK Chui","year":"1998","journal-title":"Blood"},{"key":"pone.0267832.ref027","doi-asserted-by":"crossref","first-page":"e0115960","DOI":"10.1371\/journal.pone.0115960","article-title":"Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples","volume":"10","author":"A Psifidi","year":"2015","journal-title":"PLoS One"},{"key":"pone.0267832.ref028","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/03630269.2018.1488723","article-title":"Development of visual detection of \u03b1-thalassemia-1 (the - -SEA Deletion) using pH-sensitive loop-mediated isothermal amplification","volume":"42","author":"S Chomean","year":"2018","journal-title":"Hemoglobin"},{"key":"pone.0267832.ref029","doi-asserted-by":"crossref","first-page":"303","DOI":"10.2147\/RMHP.S241399","article-title":"Establishment and evaluation of a novel method based on loop-mediated isothermal amplification for the rapid diagnosis of thalassemia genes","volume":"13","author":"WH Wang","year":"2020","journal-title":"Risk Manag Healthc Policy"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0267832","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T03:04:37Z","timestamp":1727060677000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0267832"}},"subtitle":[],"editor":[{"given":"J Francis","family":"Borgio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,4,28]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0267832","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,28]]}}}