{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:08:36Z","timestamp":1784729316699,"version":"3.55.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Tobacco Company Science and Technology Plan Project","award":["2020530000242026"],"award-info":[{"award-number":["2020530000242026"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Tobacco black shank is a kind of soil-borne disease caused by the Oomycete Phytophthora parasitica. This disease is one of the most destructive diseases to tobacco (Nicotiana tabacum L.) growth worldwide. At present, various measures have been taken to control this disease, but they still have different challenges and limitations. Studies have shown that \u03b2-aminobutyric acid (BABA), a nonprotein amino acid, can enhance disease resistance in plants against different varieties of pathogens. However, it is unclear whether BABA can induce plants to resist <jats:italic>Phytophthora parasitica<\/jats:italic> infection. Therefore, this study aims to explore the effect and related mechanism of BABA against tobacco black shank. Our results showed that 5 mmol<jats:sup>.<\/jats:sup>L<jats:sup>-1<\/jats:sup> BABA had an obvious anti-inducing effect on the pathogenic fungus and could effectively inhibit the formation of dark spots in the stems. The results also showed that a large amount of callose deposition was observed in BABA-treated tobacco. Furthermore, the application of BABA induced the accumulation of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in tobacco and effectively regulated the homeostasis of reactive oxygen in tobacco plants, reducing the toxicity of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> to plants while activating the defense system. In addition, BABA spray treatment could induce an increase in the concentrations of salicylic acid (SA) and jasmonic acid-isoleucine (JA-Ile) in tobacco, and the gene expression results confirmed that BABA upregulated the expression of SA-related genes (<jats:italic>PR1<\/jats:italic>, <jats:italic>PR2<\/jats:italic> and <jats:italic>PR5<\/jats:italic>), JA-related genes (<jats:italic>PDF1<\/jats:italic>.<jats:italic>2<\/jats:italic>) and ET-related genes (<jats:italic>EFE26<\/jats:italic> and <jats:italic>ACC oxidase<\/jats:italic>) in tobacco plants. Taken together, BABA could activate tobacco resistance to black shank disease by increasing H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> accumulation, callose deposition, plant hormone (SA and JA-Ile) production, and SA-, JA-, and ET- signaling pathways.<\/jats:p>","DOI":"10.1371\/journal.pone.0267960","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T17:41:07Z","timestamp":1654796467000},"page":"e0267960","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["\u03b2-aminobutyric acid (BABA)-induced resistance to tobacco black shank in tobacco (Nicotiana tabacum L.)"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1601-0399","authenticated-orcid":true,"given":"Xiyue","family":"Ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faliang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianwen","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"issue":"3","key":"pone.0267960.ref001","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1365-313X.2010.04436.x","article-title":"Priming for enhanced defence responses by specific inhibition of the Arabidopsisresponse to coronatine","volume":"65","author":"CH Tsai","year":"2011","journal-title":"The Plant Journal"},{"issue":"7","key":"pone.0267960.ref002","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1271\/bbb.50677","article-title":"Induction of resistance against rice blast fungus in rice plants treated with a potent elicitor, N-acetylchitooligosaccharide","volume":"70","author":"S Tanabe","year":"2006","journal-title":"Bioscience, Biotechnology, and Biochemistry"},{"issue":"3","key":"pone.0267960.ref003","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1093\/aob\/mcg151","article-title":"Regulation of jasmonate-mediated plant responses in Arabidopsis","volume":"92","author":"A Devoto","year":"2003","journal-title":"Annal of Botany"},{"issue":"4","key":"pone.0267960.ref004","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.pbi.2004.05.006","article-title":"NPR1: the spider in the web of induced resistance signaling pathways","volume":"7","author":"CM Pieterse","year":"2004","journal-title":"Current Opinion in Plant Biology"},{"issue":"1","key":"pone.0267960.ref005","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1046\/j.1365-3059.2003.00792.x","article-title":"Effects of benzothiadiazole and acetylsalicylic acid on \u03b2-1,3-glucanase activity and disease resistance in potato","volume":"52","author":"AI Bokshi","year":"2003","journal-title":"Plant Pathology"},{"key":"pone.0267960.ref006","article-title":"Sucrose metabolism and sensory evaluation in peach as influenced by \u03b2-aminobutyric acid (BABA)-induced disease resistance and the transcriptional mechanism involved","volume":"174","author":"C Li","year":"2021","journal-title":"Postharvest Biology and Technology"},{"key":"pone.0267960.ref007","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0885-5765(05)80030-X","article-title":"3-Aminobutyric acid induces system resistance against Peronospora tabaina","volume":"44","author":"Y. 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